Journal
VETERINARY PARASITOLOGY
Volume 303, Issue -, Pages -Publisher
ELSEVIER
Keywords
AgNOR; canine Leishmaniosis; renal impairment; kidney; NOR
Categories
Funding
- Fundacao de Amparo a Pesquisa do Estado de Minas Gerais - FAPEMIG
- Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior-CAPES
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico - CNPq
- CAPES (Programa de Posgraducao em Biologia Celular - ICB/UFMG)
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologica (CNPq), Brazil [168270/2017-0]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq), Brazil [308344/2016-2]
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Hyperactivation of tubular cells contributes to the progression of kidney lesions, and Leishmania-infected dogs showed higher histopathological lesion scores and AgNOR expression, which were associated with clinical signs.
Hyperactivation of tubular cells contributes for the progression of kidney lesions. The exacerbated expression of immunological proteins and ribosomal DNA (rDNA) transcriptional activity are observed in tubular cells. This intensified expression results in more prominent hypertrophic changes and is often accompanied by increased expression of factors involved in different phases of ribosomal biosynthesis, such as the nucleolar organizer regions (NOR). The aim of this study was to evaluate whether there is an association between NOR proteins, renal impairment, and clinical status in Leishmania-infected dogs (CanL). Forty-five dogs with CanL and six uninfected controls were assessed in this study. PCR was performed to detect parasites' nucleic acids in kidney. Histopathological analyses were performed in kidney fragments, and NOR was detected by Ag stain (AgNOR). Leishmania-infected dogs showed more intense inflammation and collagen deposition compared with uninfected controls. Biochemical alterations were observed only in Leishmania-infected dogs. AgNORs per cell were significantly higher in clinically affected dogs and higher histopathological lesion score was observed it Leishmania-infected dogs. Positive correlations between number of NORs per cell in medullary region and histopathological lesion score were observed. Furthermore, AgNOR expression, intensity of renal lesions, and clinical sigs was associated in Leishmania-infected dogs. We propose that the detection of AgNOR proteins could be used to better estimate the kidney tubular damage at the time of examination in Leishmania-infected dogs as a marker to estimate renal impairment in dogs with CanL.
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