Journal
ACS SENSORS
Volume 1, Issue 11, Pages 1295-1300Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acssensors.6b00352
Keywords
fluorescence blinking; quantum dots; lattices; biosensors; K-ras; nucleic acid engineering; strand displacement
Funding
- UNC Charlotte
- NSF [CMMI-1150682]
- Federal funds from the Frederick National Laboratory for Cancer Research, National Institutes of Health [HHSN26120080001E]
- Intramural Research Program of the NIH, National Cancer Institute, Center for Cancer Research
- Directorate For Engineering
- Div Of Civil, Mechanical, & Manufact Inn [1150682] Funding Source: National Science Foundation
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We demonstrate the first biosensing strategy that relies on quantum dot (QD) fluorescence blinking to report the presence of a target molecule. Unlike other biosensors that utilize QDs, our method does not require the analyte to induce any fluorescence intensity or color changes, making it readily applicable to a wide range of target species. Instead, our approach relies on the understanding that blinking, a single particle phenomenon, is obscured when several QDs lie within the detection volume of a confocal microscope. If QDs are engineered to aggregate when they encounter a particular target molecule, the observation of quasi-continuous emission should indicate its presence. As proof of concept, we programmed DNAs to drive rapid isothermal assembly of QDs in the presence of a target strand (oncogene K-ras). The assemblies, confirmed by various gel techniques, contained multiple QDs and were readily distinguished from free QDs by the absence of blinking.
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