Synthesis, anticancer activities and experimental-theoretical DNA interaction studies of 2-amino-4-phenyl-4H-benzo[h] chromene-3-carbonitrile

Twenty chromenes were synthesized with good to excellent yields (70-96%). From the series of chromenes herein tested, compounds 14, 12, 15, 16, 17, 18, and 20 were the most potent throughout the cancer human cell lines tested. In general, the para-position electron-withdrawing substituents on the phenyl ring are favored towards potency and selectivity for cancer cells. Biophysical studies were performed with eight chromene derivatives (13- 20) and ctDNA to evaluate possible biological targets. The molecular fluorescence verified that compound 16 presented a higher binding constant (Kb) with the ctDNA, agreeing with in vitro biological results and that evaluated chromenes derivatives interact preferentially via intercalation. Finally, an inverse linear correlation (logKb vs. GI50) was observed for six human carcinogenic cell lines; hence, the mechanism of action of these compounds may be related to DNA interaction.

Automated summary

Twenty chromenes were synthesized with good to excellent yields (70-96%). Compounds 14, 12, 15, 16, 17, 18, and 20 showed the strongest inhibitory effects on human cancer cell lines. The presence of para-position electron-withdrawing substituents on the phenyl ring favored potency and selectivity for cancer cells. Biophysical studies indicated that certain chromene derivatives preferentially interacted with ctDNA via intercalation, which was consistent with the in vitro biological results.