Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 20, Issue 16, Pages 3359-3364Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2ob00260d
Keywords
-
Categories
Funding
- National Natural Science Foundation of China [22177103, 21472172]
Ask authors/readers for more resources
In this study, a near-infrared fluorescent probe (FNN) was reported for sensitively detecting intracellular viscosity and N2H4 levels. The probe showed a fast and selective fluorescence response to N2H4 and could track viscosity without interference from other factors. The successful application of the probe in living cells and zebrafish larvae and embryos highlights its importance in assessing liver injury.
Hydrazide drugs can cause severe drug-induced liver injury owing to the enzymatic release of N2H4 in the liver. Also, changes in cellular viscosity are associated with liver damage. Thus, simultaneous monitoring of changes in N2H4 levels and viscosity can be used to evaluate the side effects of hydrazide drugs. Herein, we firstly reported a near-infrared fluorescent probe (FNN), which contains 1,8-naphthalimide as the fluorophore and a chalcone moiety as the responsive receptor, for sensitively detecting intracellular viscosity and N2H4. FNN showed a fast 'turn-on' fluorescence response to N2H4 with excellent selectivity. Additionally, FNN could selectively track viscosity without interference from polarity, pH, and other active species. Furthermore, imaging experiments suggested that FNN could be successfully applied in living cells and zebrafish larvae and embryos, which is of great importance for effectively assessing the degree of liver injury.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available