Journal
BREAST CANCER-BASIC AND CLINICAL RESEARCH
Volume 16, Issue -, Pages -Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/11782234221086728
Keywords
Nanoparticles; quercetin; magnetic oxide; apoptosis; radiotherapy; methyl-N-nitrosourea; breast cancer
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Quercetin-conjugated magnetite nanoparticles (QMNPs) were successfully prepared using a biological simple nanoprecipitation approach mediated by fungus Aspergillus oryzae. In vitro studies demonstrated the inhibitory effects of QMNPs on MCF-7, HePG-2, and A459 cancer cell lines, while in vivo experiments showed their potential to sensitize breast cancer to radiotherapy. The combination of QMNPs with irradiation effectively blocked cancer cell proliferation and enhanced the therapeutic outcome in a rat model of breast cancer induced by N-methyl-N-nitrosourea, suggesting the potential of QMNPs as a targeted therapy in breast cancer.
Quercetin is a potent cancer therapeutic agent present in fruits and vegetables. The pharmaceutical uses of quercetin are limited due to many problems associated with low solubility, bioavailability, permeability, and instability. In addition. the high doses of quercetin show toxic effects in clinical and experimental studies. Therefore, a new strategy is warranted to overcome these problems without the use of toxic doses. The iron oxide nanoparticles can be used as a drug delivery system. This study aimed to prepare quercetin-conjugated magnetite nanoparticles (QMNPs) using biological simple nanoprecipitation and mediated by fungus Aspergi//us oryzae. Also. we initiated in vitro and in vivo studies to determine whether QMNPs might sensitize breast cancer to radiotherapy treatment. The structural. morphological, and magnetic properties of the prepared nanoparticles were studied. The results indicated that QMNPs were spherical in shape and 40 nm in diameter. The in vitro studies showed that the incubation of MCF-7, HePG-2, and A459 cancer cells with QMNPs for 24 h effectively inhibited the growth of cancer cell lines in a concentration-dependent manner with IC50 values of 11, 77.5, and104nmol/mL, respectively. The combination of QMNPs with irradiation (IR) potently blocked MCF-7 cancer cell proliferation and showed significant changes in the morphology of these cells as observed by bright-field inverted light microscopy. Focusing on the long-term toxicity of QMNPs (20ml/kg). the assessment of hematological. hepatic. and renal markers indicated no toxic effect. Besides. QMNPs inhibited tumor growth and potently enhanced the lateral radiotherapy treatment in N-methyl-N-nitrosourea (MNU)-induced breast cancer in female white albino rats. These anticancer and radiosensitizing activities were ascribed to cytotoxicity, cell cycle arrest, immunomodulation, and efficiency through induction of apoptosis. In a conclusion, these observations suggest that the QMNPs combined with LRT could act as a potential targeted therapy in breast cancer.
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