Journal
GELS
Volume 8, Issue 3, Pages -Publisher
MDPI
DOI: 10.3390/gels8030182
Keywords
nanohydrogels-hyaluronic acid-HA-mPEG(2000); fluorinated and fluorescent HA conjugates-hyaluronic acid degree of substitution-diffusion ordered spectroscopy (DOSY)-1D diffusion-filtered F-19 NMR-atomic force microscopy-FRET experiments-hyaluronidase-nanohydrogel stability
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Funding
- Programme de cooperation transfrontaliere Interreg France-Wallonie-Vlaanderen
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Hyaluronic acid was functionalized with different amino synthons, and nanohydrogels were prepared via ionic gelation. The stability of these nano-assemblies was evaluated using FRET experiments.
Hyaluronic acid (HA) was functionalized with a series of amino synthons (octylamine, polyethylene glycol amine, trifluoropropyl amine, rhodamine). Sodium hyaluronate (HAs) was first converted into its protonated form (HAp) and the reaction was conducted in DMSO by varying the initial ratio (-NH2 (synthon)/COOH (HAp)). HA derivatives were characterized by a combination of techniques (FTIR, H-1 NMR, 1D diffusion-filtered F-19 NMR, DOSY experiments), and degrees of substitution (DSHA) varying from 0.3% to 47% were determined, according to the grafted synthon. Nanohydrogels were then obtained by ionic gelation between functionalized hyaluronic acids and chitosan (CS) and tripolyphosphate (TPP) as a cross-linker. Nanohydrogels for which HA and CS were respectively labeled by rhodamine and fluorescein which are a fluorescent donor-acceptor pair were subjected to FRET experiments to evaluate the stability of these nano-assemblies.
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