OsNPF5.16, a nitrate transporter gene with natural variation, is essential for rice growth and yield

Rice has a large number of nitrate or peptide transporter family (NPF) genes, but the effects of most members on rice growth and development are unknown. We report that OsNPF5.16, a nitrate transporter gene with natural variation in its promoter sequence, is essential for rice growth and yield. The promoter sequence showed various differences between indica and japonica cultivars, and higher expression of OsNPF5.16 was found in indica cultivars with higher plant weight and more tillers than japonica cultivars. OsNPF5.16 was highly expressed in roots, tiller basal parts, and leaf sheaths, and its protein was localized on the plasma membrane. In cRNA-injected Xenopus laevis oocytes, OsNPF5.16 transport of nitrate at high nitrate concentration depended on pH. Overexpression of OsNPF5.16 increased nitrate content and total nitrogen content in leaf sheath as well as biomass and tiller bud length in rice. Elevated expression of OsNPF5.16 increased rice tiller number and grain yield by regulating cytokinin levels. Inhibition of OsNPF5.16 expression showed the opposite effects. Regulating OsNPF5.16 expression has potential for improving rice grain yield. (C) 2021 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Automated summary

This study finds that OsNPF5.16 gene, a nitrate transporter gene in rice, plays an essential role in rice growth and yield. Higher expression of OsNPF5.16 is associated with increased plant weight, tiller number, and grain yield in indica cultivars compared to japonica cultivars. Overexpression of OsNPF5.16 increases nitrate content, total nitrogen content, biomass, and tiller bud length in rice. Regulating the expression of OsNPF5.16 shows potential for improving rice grain yield.