Journal
AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
Volume 322, Issue 4, Pages F379-F391Publisher
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajprenal.00326.2021
Keywords
Cre-LoxP system; development; in vivo lineage tracing; regeneration; renal stem/progenitor cells
Categories
Funding
- National Institute of Diabetes and Digestive and Kidney Diseases Grant [DK080236]
Ask authors/readers for more resources
This article summarizes the recent advances in utilizing the Cre-LoxP system for tracking stem/progenitor cells in kidney development and maturity, as well as the development and characterization of various Cre drivers.
Mammalian kidneys consist of more than 30 different types of cells. A challenging task is to identify and characterize the stem/progenitor subpopulations that establish the lineage relationships among these cellular elements during nephrogenesis in the embryonic and neonate kidneys and during tissue homeostasis and/or injury repair in the mature kidney. Moreover, the potential clinical utility of stem/progenitor cells holds promise for the development of new regenerative medicine approaches for the treatment of renal diseases. Stem cells are defined by unlimited self-renewal capacity and pluripotentiality. Progenitor cells have pluripotentiality but no or limited self-renewal potential. Cre-LoxP-based in vivo genetic lineage tracing is a powerful tool to identify stem/progenitor cells in their native environment. Hypothetically, this technique enables investigators to accurately track the progeny of a single cell or a group of cells. The Cre/LoxP system has been widely used to uncover the function of genes in various mammalian tissues and to identify stem/progenitor cells through in vivo lineage tracing analyses. In this review, we summarize the recent advances in the development and characterization of various Cre drivers and their use in identifying potential renal stem/progenitor cells in both developing and mature mouse kidneys.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available