Effects of Microbial Inoculation with Different Indigenous Bacillus Species on Physicochemical Characteristics and Bacterial Succession during Short-Term Composting

Bacillus accelerates lignocellulose degradation, promotes the stabilization and resource utilization of compost by secreting enzymes, and plays an important role in compost formation and quality control. This study evaluated enzyme activity, lignocellulosic degradation, and bacterial succession in composting inoculated with different microbial Bacillus agents. They were identified as B. licheniformis, B. subtilis, B. thermoamylovorans, B. thermoruber, and B. hisashii. Four treatments were established, including a CK (uninoculated microorganisms), A (B. licheniformis, B. subtilis, B. thermoamylovorans,and B. hisashii), B (B. subtilis, B. thermoamylovorans, B. thermoruber, and B. hisashii), and C (B. subtilis, B. thermoamylovorans, and B. hisashii), and the composting lasted 7-14 days. Lignin and cellulose degradation rates in B during composting were 17.1% and 36.7% at the cooling stage, respectively. Redundancy analysis showed that degradation of lignocellulose in the thermophilic stage was mainly related to the secretion of lignocellulose-degrading enzymes after microbial inoculation. 16S rRNA sequencing revealed that Bacillus (20.3%) and Thermobifida (20.2%) were the dominant genera. Inoculation with a combination including B. thermoruber was a feasible way to increase lignocellulose degradation and promote maturity in sewage sludge composting.

Automated summary

Bacillus accelerates lignocellulose degradation in compost and plays a crucial role in compost formation and quality control. In this study, different microbial Bacillus agents were inoculated into compost, resulting in increased lignocellulosic degradation, especially with the inclusion of B. thermoruber.