Journal
THERANOSTICS
Volume 12, Issue 7, Pages 3131-3149Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.69217
Keywords
Olig2; Sonic hedgehog (Shh); Oligodendrocyte progenitor cells (OPCs); Human induced pluripotent stem cells (hiPSCs); Ischemic stroke
Categories
Funding
- Shenzhen Science and Technology Program [KQTD20190929173853397]
- National Natural Science Foundation of China [81971081]
- open project of State Key Laboratory of Respiratory Disease [SKLRD-OP-201905]
- project of Dengfeng Plan High-level Hospital Construction Opening Project of Foshan First People's Hospital [FSSYKF-2020010]
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By using a chemically defined protocol, we were able to generate a substantial number of transplantable and functional OLs from hiPSCs, which holds promise for cell-based therapy in ischemic stroke.
Rationale: Demyelination is a major component of white matter injury, characterized by oligodendrocyte (OL) death and myelin sheath loss, which result in memory loss and cognitive impairment in the context of ischemic stroke. Accumulating evidence has shown that OLs can be generated by the direct activation of defined transcription factors (TFs) in human induced pluripotent stem cells (hiPSCs); however, the rapid acquisition of single TF-induced OL progenitor cells (OPCs) as cell therapy for ischemic stroke remains to be thoroughly explored. Methods: A stable, chemically defined protocol was used to generate a substantial number of transplantable and functional OLs through the partial inhibition of sonic hedgehog (Shh) activity by GANT61 during neural induction from hiPSCs and sequential induction of TF Olig2 overexpression. Transcriptome and metabolome analyses further revealed a novel molecular event in which Olig2 regulates OL differentiation from hiPSC-derived neural progenitor cells (NPCs). Olig2-induced NG2(+) OPCs (Olig2-OPCs) were then evaluated for their therapeutic potential in cell-based therapy for ischemic stroke. Results: GANT61 treatment resulted in a motor neuron (MN)-OL fate switch during neural induction, and induced overexpression of Olig2 accelerated oligodendroglial lineage cell specification. Olig2-OPCs expressed typical oligodendroglial lineage marker genes, including NKX2.2, CSPG4, and ST8SIA 1, and displayed superior ability to differentiate into mature OLs in vitro. Mechanistically, Olig2-OPCs showed increased gene expression of the peroxisome proliferator-activated receptor gamma (PPAR gamma) signaling pathway, and activated CEPT1 -mediated phospholipogenesis. Functionally, inhibiting PPAR gamma and knocking down CEPT1 further compromised the terminal differentiation of Olig2-OPCs. Most importantly, when transplanted into a rat model of transient middle cerebral artery occlusion (tMCAO), Olig2-OPCs efficiently promoted neurological functional recovery by reducing neuronal death, promoting remyelination, and rescuing spatial memory decline. Conclusions: We developed a stable, chemically defined protocol to generate OPCs/OLs with partial inhibition of Shh activity by GANT61 from hiPSCs and sequentially induced the expression of the single TF Olig2. Olig2-OPC transplantation may be an ideal alternative approach for ischemic stroke rehabilitation therapy.
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