The Measurement, Application, and Effect of Oxygen in Microbial Fermentations: Focusing on Methane and Carboxylate Production

Oxygen is considered detrimental to anaerobic fermentation processes by many practitioners. However, deliberate oxygen sparging has been used successfully for decades to remove H 2 S in anaerobic digestion (AD) systems. Moreover, microaeration techniques during AD have shown that small doses of oxygen may enhance process performance and promote the in situ degradation of recalcitrant compounds. However, existing oxygen dosing techniques are imprecise, which has led to inconsistent results between studies. At the same time, real-time oxygen fluxes cannot be reliably quantified due to the complexity of most bioreactor systems. Thus, there is a pressing need for robust monitoring and process control in applications where oxygen serves as an operating parameter or an experimental variable. This review summarizes and evaluates the available methodologies for oxygen measurement and dosing as they pertain to anaerobic microbiomes. The historical use of (micro-)aeration in anaerobic digestion and its potential role in other anaerobic fermentation processes are critiqued in detail. This critique also provides insights into the effects of oxygen on these microbiomes. Our assessment suggests that oxygen dosing, when implemented in a controlled and quantifiable manner, could serve as an effective tool for bioprocess engineers to further manipulate anaerobic microbiomes for either bioenergy or biochemical production.

Automated summary

Many practitioners believe that oxygen is detrimental to anaerobic fermentation processes, but intentional oxygen sparging can effectively remove H2S in anaerobic digestion systems. Additionally, microaeration techniques have shown that small doses of oxygen can enhance process performance and promote the degradation of recalcitrant compounds. However, existing oxygen dosing techniques are imprecise, highlighting the need for robust monitoring and process control.