Journal
ANALYTICAL METHODS
Volume 14, Issue 18, Pages 1803-1809Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2ay00368f
Keywords
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Funding
- National Natural Science Foundation of China [21864018, 22164013]
- Natural Science Foundation of Jiangxi Province [20212BAB203033]
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A colorimetric assay based on gold nanoparticle aggregation and in situ silver metallization was developed for determination of tetracyclines in complex matrices. The assay utilized aptamer-functionalized gold nanoparticles to catalyze a reaction and amplify the signal through the interaction between the target and aptamer.
A colorimetric assay was described for determination of tetracyclines (TCs) in complex matrices based on dual regulation of gold nanoparticle (AuNP) aggregation and in situ silver metallization. The reaction between p-aminophenol and silver ions (Ag+) catalyzed by aptamer (Apt)-functionalized AuNPs promoted the in situ deposition of Ag shells on AuNPs to afford core-shell bimetallic NPs (Au@AgNPs). When the target is present, Apt is separated from the AuNP surface through the Apt-target interaction. With the desorption of Apts, AuNPs catalyzed the formation of more aggregated Au@AgNPs with a thinner Ag shell, corresponding to the solution color gradually changing from orange-yellow to brown. Using A(600)/A(410) as the determination signal, the assay gave visible linear relationships for TCs in the range of 0.3-6.0 mu mol L-1 with a detection limit (LOD) of 33.6 nmol L-1. In particular, the assay was successfully applied to detect TCs in real samples with the recovery rate ranging from 91.2% to 106% and relative standard deviation (RSD) less than 4.6%. These results meet the requirements of sample analysis.
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