HPLC-ESI-MSn Analysis and Validation of UV-Vis and RP-HPLC-PDA Methods for Polyphenols Quantification from Hymenaea eriogyne

The validation of analytical methods for the quantification of chemical markers of Hymenaea eriogyne Benth., Fabaceae, is a fundamental step toward providing standardized extracts and improving the quality of herbal medicines. Qualitative profiles of the extracts were obtained by HPLC-ESI-MSn. Furthermore, analytical methods were optimized and validated using UV-Vis spectroscopy and RP-HPLC-PDA, according to international guidelines. Considering the experimental design, the best conditions for total polyphenol content, dry residue, and extraction efficiency were achieved using turbo extraction and 10% (w/v). Acetone to water (7:3; v/v) mixture was found to be the ideal solvent in this study, yielding total polyphenol content of 11.38 g% (0.89%) and 10.41 g% (1.69%) for the stem barks and leaves, respectively. HPLC-ESI-MSn analysis allowed the identification of phenolic compounds, most of them flavonoids, such as rutin and astilbin. The chromatographic and spectrophotometric analyses demonstrated robustness; both methods showed linearity (R-2 > 0.99), selectivity, specificity, precision (RSD < 4.1%), and accuracy. The methods are simple and can be used for the quantification of compounds from H. eriogyne. Therefore, our study represents an important step in the quality analysis and technological development of the species.

Automated summary

The validation of analytical methods for the quantification of chemical markers of Hymenaea eriogyne Benth., Fabaceae is important for standardized extracts and improving herbal medicine quality. The optimal conditions for extraction were determined to be turbo extraction and a 10% (w/v) acetone-water (7:3; v/v) mixture. HPLC-ESI-MSn analysis identified phenolic compounds, primarily flavonoids. The validated methods demonstrated robustness and accuracy for quantifying compounds from H. eriogyne.