UV-Crosslinked Electrospun Zein/PEO Fibroporous Membranes for Wound Dressing

Electrospun zein membranes are suitable for various biomedical applications. A UV-crosslinked electrospun membrane of a zein/PEO blend for wound healing application was explored in this work. The improvement in mechanical properties of the membrane after UV crosslinking was attributed to the change in protein conformation from an alpha-helix to a beta-sheet The circular dichroism (CD) spectra and FTIR spectra confirmed this conformational change. XRD analysis was shown to prove the amorphous nature of polymer blends with specific broad peaks at 2 theta = 9 degrees and 20 degrees. The water vapor transmission rate (WVTR) of the membrane was found to be in the range of 1500-2000 g m(-2)day(-1), which was well suited with that of commercially available wound dressing material. Enough number of available functional groups like thiol, amino, and hydroxyl groups supplement a blood dotting index (BCI) to the matrix, causing 99% BCI within 4 min. A 91% cell viability result in the MTT assay with human dermal fibroblast cells confirmed the noncytotoxicity of the membrane. Tripeptides produced after the thermolysin-based hydrolysis of zein caused inhibition of TGF beta 1 expression and thus increased fibroblast and collagen production. The membrane stimulated 54% more collagen production compared to control cells at day 2 and caused 84% wound dosure in human dermal fibroblast cells, which were desirable index markers of a potential wound care material.

Automated summary

Electrospun zein/PEO membranes were modified by UV crosslinking, leading to improved mechanical properties and noncytotoxicity. The membranes promoted collagen production and wound closure, making them a promising material for wound healing applications.