Journal
NANOBIOTECHNOLOGY REPORTS
Volume 17, Issue 1, Pages 106-117Publisher
PLEIADES PUBLISHING INC
DOI: 10.1134/S2635167622010025
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- Ministry of Science and Higher Education of the Russian Federation [FEWG-2020-0008]
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The possibility of using redox-active polymers based on bovine serum albumin and chitosan covalently linked to neutral red, thionine, and ferrocene mediators, and containing carbon nanotubes for glucose-oxidase immobilization is investigated. The systems containing a redox-active polymer based on BSA modified with a ferrocene mediator are found to be the most promising. Biosensors formed using nanocomposite matrices have a high sensitivity and high correlation with the results of determining the glucose content in human blood by the standard method.
The possibility of using redox-active polymers based on bovine serum albumin (BSA) and chitosan covalently linked to neutral red, thionine, and ferrocene mediators, and containing carbon nanotubes (CNTs) for glucose-oxidase immobilization is investigated. The structure of the obtained polymers is studied by infrared (IR) spectroscopy, spectrophotometry, atomic-absorption spectroscopy, and scanning electron microscopy. Using the method of cyclic voltammetry, the electrochemical characteristics of the studied conductive nanocomposite systems are found, namely, a heterogeneous rate constant of electron transfer and a rate constant of interaction with glucose oxidase. The systems containing a redox-active polymer based on BSA modified with a ferrocene mediator turn out to be the most promising. Biosensors formed using nanocomposite matrices have a high sensitivity with a lower limit of determined glucose concentrations of 0.1 mM and are characterized by a high correlation (R (2) = 0.9827) with the results of determining the glucose content in human blood by the standard method.
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