Journal
ANALYST
Volume 147, Issue 12, Pages 2731-2738Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2an00222a
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Funding
- Paul Manning Launchpad Award
- US AFOSR [FA2386-21-1-4070]
- University of Virginia's Cancer Center through the NCI Cancer Center Support Grant [P30 CA44579]
- University of Virginia's Advanced Biomanufacturing and Engineering-in-Medicine initiatives
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Islet transplantation is an expensive treatment for type 1 diabetes due to limited pancreas donors and variability in islet quality. Co-culturing islets with stem cells can promote remodeling and angiogenesis, but there is a need for metrics to assess islet reorganization. Biomechanical metrics based on aggregate shape evolution and microfluidic constriction pressures can monitor reorganization dynamics over time.
Islet transplantation is a potential therapy for type 1 diabetes, but it is expensive due to limited pancreas donor numbers and the variability in islet quality. The latter is often addressed by co-culture of harvested islets with stem cells to promote in vitro remodeling of their basement membrane and enable expression of angiogenic factors for enhancing vascularization. However, given the heterogeneity in islet size, shape and function, there is a need for metrics to assess the reorganization dynamics of single islets over the co-culture period. Based on shape-evolution of individual multi-cell aggregates formed during co-culture of human islets with adipose derived stem cells and the pressures required for their bypass through microfluidic constrictions, we present size-normalized biomechanical metrics for monitoring the reorganization. Aggregates below a threshold size exhibit faster reorganization, as evident from rise in their biomechanical opacity and tightening of their size distribution, but this size threshold increases over culture time to include a greater proportion of the aggregates. Such biomechanical metrics can quantify the subpopulation of reorganized aggregates by distinguishing them versus those with incomplete reorganization, over various timepoints during the co-culture.
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