Journal
FOOD QUALITY AND SAFETY
Volume 6, Issue -, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/fqsafe/fyac007
Keywords
Penconazole; monoclonal antibody; lateral flow immunoassay; watermelon; cucumber
Categories
Funding
- National Key RD Program [2019YFC1604604]
- Suzhou Science and Technology Committee Program [SS202038]
- Postgraduate Research & Practice Innovation Program of Jiangsu Province [KYCX20_1841]
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A new hapten was designed to develop a highly sensitive and specific anti-penconazole monoclonal antibody (mAb), and a gold nanoparticle-based lateral-flow immunoassay (LFIA) was established for the rapid detection of penconazole residues in watermelon and cucumber.
As a typical triazole fungicide, penconazole (PEN) is widely used in agriculture but has been proven to be toxic. In this study, we designed a new hapten to prepare a highly sensitive and specific anti-PEN monoclonal antibody (mAb) and established a gold nanoparticle-based lateral-flow immunoassay (LFIA) for the detection of PEN residues in watermelon and cucumber. The 50% inhibitory concentration (IC50) of the mAb was 0.42 ng/mL and the LFIA strip had a visual limit of detection (vLOD) of 2.5 ng/g and a cut-off value of 10 ng/g in watermelon and cucumbers. The calculated limit of detection (LOD) of the LFIA strip was 0.36 ng/g for watermelon and 0.29 ng/g for cucumber. The LFIA strip also gave a recovery rate of 92.5%-109.0% for watermelon samples and 92.5%-106.7% for cucumber samples. These results using the LFIA strip are highly consistent with those seen using liquid chromatography-tandem mass spectrometry. Thus our developed LFIA strip represents a potentially reliable tool for the rapid on-site screening for PEN in watermelons and cucumbers.
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