Journal
FACETS
Volume 7, Issue -, Pages 774-791Publisher
CANADIAN SCIENCE PUBLISHING
DOI: 10.1139/facets-2021-0166
Keywords
insulin sensitivity; glucose transport; AMP-activated protein kinase; aging; cholesterol; skeletal muscle
Categories
Funding
- National Institutes of Health [AG010026]
Ask authors/readers for more resources
AICAR treatment can increase γ3-AMPK activity and reduce membrane cholesterol content, leading to enhanced glucose uptake in aged skeletal muscles. This study provides insights into potential mechanisms for improving glucose metabolism in older individuals.
Attenuated skeletal muscle glucose uptake (GU) has been observed with advancing age. It is important to elucidate the mechanisms linked to interventions that oppose this detrimental outcome. Earlier research using young rodents and (or) cultured myocytes reported that treatment with 5-aminoimidazole-4-carboxamide-1-beta-d ribofuranoside (AICAR; an AMP-activated protein kinase (AMPK) activator) can increase gamma 3-AMPK activity and reduce membrane cholesterol content, each of which has been proposed to elevate GU. However, the effect of AICAR treatment on gamma 3-AMPK activity and membrane cholesterol in skeletal muscle of aged animals has not been reported. Our purpose was to evaluate the effects of AICAR treatment on these potential mechanisms for enhanced glucose uptake in the skeletal muscle of aged animals. Epitrochlearis muscles from 26-27-month-old male rats were isolated and incubated +/- AICAR, followed by 3 h incubation without AICAR, and then incubation with 3-O-methyl-[(3) H] glucose (to assess GU +/- insulin). Muscles were also analyzed for gamma 3-AMPK activity and membrane cholesterol content. Prior AICAR treatment led to increased gamma 3-AMPK activity, reduced membrane cholesterol content, and enhanced glucose uptake in skeletal muscle from aged rats. These observations revealed that two potential mechanisms for greater GU previously observed in younger animals and (or) cell models are also potentially relevant for enhanced GU by muscles from older animals.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available