Journal
FOOD & FUNCTION
Volume 13, Issue 11, Pages 6008-6021Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2fo00729k
Keywords
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Funding
- Key Research and Development Program of Science and Technology Department of Sichuan Province [2020YFS0570]
- project of Medical Science and Technology of Health Commission of Sichuan Province [21PJ064]
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Ocean life contains bioactive peptides with potential application in nutraceuticals and pharmaceuticals. This study identified neuroprotective antioxidant peptides in sea squirt (Halocynthia roretzi) and demonstrated their potential to alleviate neurodegenerative diseases associated with oxidative stress. The peptide WLP showed high oxygen radical absorbance capacity and effectively increased cell viability, attenuated cell apoptosis, and decreased reactive oxygen species levels.
Ocean life contains a wealth of bioactive peptides that could be utilized in nutraceuticals and pharmaceuticals. This study aimed to obtain neuroprotective antioxidant peptides in sea squirt (Halocynthia roretzi) through protamex enzymolysis. Fraction F4 (ultrafiltration generated four fractions) had a lower molecular weight (<500 Dalton (Da)) with greater 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) diammonium salt (ABTS) radical scavenging activities (94.24 +/- 2.50% and 91.80 +/- 1.19%). After gel filtration, six peptides, including Phe-Gly-Phe (FGF), Leu-Gly-Phe (LGF), Leu-Phe-VAL (LFV), Val-Phe-Leu (VFL), Trp-Leu-Pro (WLP), and Ile-Ser-Trp (ISW), were identified and sequenced by liquid chromatography-mass spectrometry (LC-MS/MS). Peptides WLP and ISW showed higher oxygen radical absorbance capacity (ORAC) values (2.72 +/- 0.47 and 1.93 +/- 0.01 mu mol L-1 of Trolox equivalent (TE) per mu mol L-1 of peptide) than glutathione (GSH). Additionally, WLP effectively increased cell viability, dramatically attenuated 6-Hydroxydopamine (6-OHDA)-induced cell apoptosis and decreased reactive oxygen species (ROS) levels to nearly two-fold, and significantly boosted glutathione peroxidase (GSH-Px) activity in PC12 cells. Transcriptome sequencing revealed differential expression of genes associated with various oxidative stress pathways after WLP treatment, such as glutathione metabolism. These results suggest that the Halocynthia roretzi-derived tripeptide WLP could alleviate neurodegenerative diseases associated with oxidative stress.
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