Journal
GELS
Volume 8, Issue 5, Pages -Publisher
MDPI
DOI: 10.3390/gels8050266
Keywords
lizardfish scale; organic acid-aided extraction; structural characteristic; biochemical property
Categories
Funding
- Ministry of Higher Education Malaysia [FRGS/1/2019/STG03/UMS/02/5]
Ask authors/readers for more resources
The research extracted collagen from lizardfish scales using various acids and found that acetic acid-extracted collagen had a higher yield and thermal stability. All extracted collagens were categorized as type I collagens with a triple-helical structure.
The purpose of this research was to extract collagen from the scales of lizardfish (Saurida tumbil) using various acids. Acetic acid-extracted collagen (AScC) produced a higher yield (1.8 mg/g) than lactic acid-extracted collagen (LScC) and citric acid-extracted collagen (CScC) although not significantly different (p > 0.05). All extracted collagens were categorized as type I collagens with the presence of alpha chains (alpha(1) and alpha(2)) based on the SDS-PAGE profiles. The triple-helical structure of the collagen was maintained in the AScC, LScC, and CScC as confirmed by the FTIR spectra. The UV-vis and X-ray diffraction spectra observed in all collagens were in agreement with previous work on fish scale and calfskin (commercial) collagens. The thermal stability of AScC (T-max = 31.61 degrees C) was greater than LScC (T-max = 30.86 degrees C) and CScC (T-max = 30.88 degrees C). The microstructure of acid-extracted collagens was characterized as complex, fibrous, and multilayered, with irregular sheet-like structures. All samples were highly soluble in acidic pH (1.0-4.0) and in low concentrations of NaCl (0-20 g/L). In conclusion, the lizardfish scale collagen, particularly AScC, may be used as an alternative to terrestrial animal collagen.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available