Journal
THERANOSTICS
Volume 12, Issue 7, Pages 3676-3689Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.72511
Keywords
cancer heterogeneity; circulating tumor cells; continuous centrifugal microfluidics; unbiased isolation; full automation
Categories
Funding
- Bio & Medical Technology Development Program of the National Research Foundation - Ministry of Science, ICT [NRF-2017M3A9E9072669]
- CTCELLS, Inc.
Ask authors/readers for more resources
Understanding cancer heterogeneity is crucial for identifying diverse genetic mutations in metastatic cancers. Isolating different types of CTCs from patients to obtain accurate cancer information is essential. The development of fully automated methods for capturing CTCs is urgently needed for routine clinical practice.
Understanding cancer heterogeneity is essential to finding diverse genetic mutations in metastatic cancers. Thus, it is critical to isolate all types of CTCs to identify accurate cancer information from patients. Moreover, full automation robustly capturing the full spectrum of CTCs is an urgent need for CTC diagnosis to be routine clinical practice. Methods: Here we report the full capture of heterogeneous CTC populations using fully automated, negative depletion-based continuous centrifugal microfluidics (CCM). Results: The CCM system demonstrated high performance (recovery rates exceeding 90% and WBC depletion rate of 99.9%) across a wide range of phenotypes (EpCAM(+), EpCAM(???), small-, large-sized, and cluster) and cancers (lung, breast, and bladder). Applied in 30 lung adenocarcinoma patients harboring epidermal growth factor receptor (EGFR) mutations, the system isolated diverse phenotypes of CTCs in marker expression and size, implying the importance of unbiased isolation. Genetic analyses of intra-patient samples comparing cell-free DNA with CCM-isolated CTCs yielded perfect concordance, and CTC enumeration using our technique was correlated with clinical progression as well as response to EGFR inhibitors. Conclusion: Our system also introduces technical advances which assure rapid, reliable, and reproducible results, thus enabling a more comprehensive application of robust CTC analysis in clinical practice.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available