Journal
CELL SYSTEMS
Volume 2, Issue 1, Pages 27-37Publisher
CELL PRESS
DOI: 10.1016/j.cels.2016.01.001
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Funding
- BBSRC Strategic LoLa grant [BB/MM00354X/1]
- BBSRC [BB/M00354X/1] Funding Source: UKRI
- Biotechnology and Biological Sciences Research Council [BB/M00354X/1] Funding Source: researchfish
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The transition from G1 into DNA replication (S phase) is an emergent behavior resulting from dynamic and complex interactions between cyclin-dependent kinases (Cdks), Cdk inhibitors (CKIs), and the anaphase-promoting complex/cyclosome (APC/C). Understanding the cellular decision to commit to S phase requires a quantitative description of these interactions. We apply quantitative imaging of single human cells to track the expression of G1/S regulators and use these data to parametrize a stochastic mathematical model of the G1/S transition. We show that a rapid, proteolytic, double-negative feedback loop between Cdk2: Cyclin and the Cdk inhibitor p27(Kip1) drives a switch-like entry into S phase. Furthermore, our model predicts that increasing Emi1 levels throughout S phase are critical in maintaining irreversibility of the G1/S transition, which we validate using Emi1 knockdown and live imaging of G1/S reporters. This work provides insight into the general design principles of the signaling networks governing the temporally abrupt transitions between cell-cycle phases.
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