Journal
CELL SYSTEMS
Volume 3, Issue 3, Pages 221-+Publisher
CELL PRESS
DOI: 10.1016/j.cels.2016.08.010
Keywords
-
Categories
Funding
- Swedish Cancer Society
- Swedish Research Council (STARGET)
- Swedish Foundation for Strategic Research
- Center for Innovative Medicine
- Ragnar Soderberg Foundation
- European Research Council [261063]
- Human Frontier Science Program
- Karolinska Institutet KID
- Knut and Alice Wallenberg Foundation
- Swedish Research Council
- European Research Council (ERC) [261063] Funding Source: European Research Council (ERC)
Ask authors/readers for more resources
The murine epidermis with its hair follicles represents an invaluable model system for tissue regeneration and stem cell research. Here we used single-cell RNA-sequencing to reveal how cellular heterogeneity of murine telogen epidermis is tuned at the transcriptional level. Unbiased clustering of 1,422 single-cell transcriptomes revealed 25 distinct populations of interfollicular and follicular epidermal cells. Our data allowed the reconstruction of gene expression programs during epidermal differentiation and along the proximal-distal axis of the hair follicle at unprecedented resolution. Moreover, transcriptional heterogeneity of the epidermis can essentially be explained along these two axes, and we show that heterogeneity in stem cell compartments generally reflects this model: stem cell populations are segregated by spatial signatures but share a common basal-epidermal gene module. This study provides an unbiased and systematic view of transcriptional organization of adult epidermis and highlights how cellular heterogeneity can be orchestrated in vivo to assure tissue homeostasis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available