Journal
REGENERATIVE BIOMATERIALS
Volume 9, Issue -, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/rb/rbac030
Keywords
acellular patches; crosslinking; functionalization; self-assembly
Categories
Funding
- National Key Research and Development Program of China [2016YFC1100900]
- National Natural Science Foundation of China [81770390, 82070402, 82170376]
- Key Research and Development Program of Ningbo [2018B10092]
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This study proposed a green strategy to crosslink and functionalize acellular scaffolds using the self-assembly of copper@tea polyphenol nanoparticles. The resulting nanocomposite acellular scaffolds, named Cu@TP-dBPs, showed comparable biomechanics and biostability to glutaraldehyde crosslinked decellularized bovine pericardias (Glut-dBPs), but also had advantages in terms of anticalcification, remodeling, and integration capabilities. Cu@TP-dBPs also possessed antibacterial and proangiogenic activities, making them promising functional acellular patches for cardiovascular applications.
Decellularization is a promising technique to produce natural scaffolds for tissue engineering applications. However, non-crosslinked natural scaffolds disfavor application in cardiovascular surgery due to poor biomechanics and rapid degradation. Herein, we proposed a green strategy to crosslink and functionalize acellular scaffolds via the self-assembly of copper@tea polyphenol nanoparticles (Cu@TP NPs), and the resultant nanocomposite acellular scaffolds were named as Cu@TP-dBPs. The crosslinking degree, biomechanics, denaturation temperature and resistance to enzymatic degradation of Cu@TP-dBPs were comparable to those of glutaraldehyde crosslinked decellularized bovine pericardias (Glut-dBPs). Furthermore, Cu@TP-dBPs were biocompatible and had abilities to inhibit bacterial growth and promote the formation of capillary-like networks. Subcutaneous implantation models demonstrated that Cu@TP-dBPs were free of calcification and allowed for host cell infiltration at Day 21. Cardiac patch graft models confirmed that Cu@TP-dBP patches showed improved ingrowth of functional blood vessels and remodeling of extracellular matrix at Day 60. These results suggested that Cu@TP-dBPs not only had comparable biomechanics and biostability to Glut-dBPs, but also had several advantages over Glut-dBPs in terms of anticalcification, remodeling and integration capabilities. Particularly, they were functional patches possessing antibacterial and proangiogenic activities. These material properties and biological functions made Cu@TP-dBPs a promising functional acellular patch for cardiovascular applications.
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