A probe-free electrochemical immunosensor for methyl jasmonate based on a Cu-MOF-carboxylated graphene oxide platform

Methyl jasmonate (MeJA) is an important phytohormone which can regulate plant growth and stress tolerance. It is very necessary to develop sensitive and accurate detection methods for MeJA. In this work, a probe-free electrochemical immunosensor for MeJA detection was developed based on a Cu-MOF-carboxylated graphene oxide (COOH-GO) platform. The Cu2+ in the Cu-MOFs was used to provide redox signals, which avoids the application of an external redox probe in the electrolyte solutions as conventional immunosensors. COOH-GO was used to improve the structural stability and provide more sites for binding MeJA antibodies. The linear range of the MeJA immunosensor is from 10 pM to 100 mu M, which can cover the whole concentration range of MeJA in most plants. And its detection limit is very low (0.35 pM), and it can detect very low concentrations of MeJA. This immunosensor is simple, low cost, and does not need redox probe solutions for measurements. It shows remarkable potential for on-site application in precision agriculture.

Automated summary

A probe-free electrochemical immunosensor based on a Cu-MOF-carboxylated graphene oxide (COOH-GO) platform was developed for methyl jasmonate (MeJA) detection. The sensor utilizes Cu2+ in Cu-MOFs to provide redox signals, eliminating the need for an external redox probe. The COOH-GO improves structural stability and increases binding sites for MeJA antibodies. The immunosensor has a wide linear range, low detection limit, and potential for on-site applications in precision agriculture.