Journal
ANIMAL NUTRITION
Volume 9, Issue -, Pages 143-158Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.aninu.2022.01.007
Keywords
Dietary exposure; Intestinal microbiota; Liver metabolomics; Microplastics; Nutrient composition; Yellow perch
Funding
- University of Wisconsin-Milwaukee (UWM) [150-25-3150-343 PRJ93WQ]
- USDA-ARS in-house project [5090-31320-004-0 0D, 5090-31320-005-0 0D]
- UW-system water research fellowship
- UWM SURF
- Naulin Foundation
- Oak Ridge Institute for Science Education
- China Scholarship Council [201803260002, 201806330033]
- Education Department of Fujian Province, China
- USAID in Egypt
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This study investigated the effects of high-density polyethylene (HDPE) on yellow perch and found that HDPE did not significantly affect the growth of the fish. However, dietary exposure to HDPE decreased the nutritional quality of the fish, altered nutrient metabolism, and affected the intestinal histopathology and microbiota community.
Microplastics are emergent contaminants threatening aquatic organisms including aquacultured fish. This study investigated the effects of high-density polyethylene (HDPE, 100 to 125 mu m) on yellow perch (Perca flavescens) based on integrative evaluation including growth performance, nutritional status, nutrient metabolism, fish health, and gut microbial community. Five test diets (0, 1, 2, 4, or 8 g HDPE/100 g diet) containing 41% protein and 10.5% lipid were fed to juvenile perch (average body weight, 25.9 +/- 0.2 g; n = 15) at a feeding rate of 1.5% to 2.0% body weight daily. The feeding trial was conducted in a flow-through water system for 9 wk with 3 tanks per treatment and 15 yellow perch per tank. No mortality or HDPE accumulation in the fish was found in any treatments. Weight gain and condition factor of fish were not significantly impacted by HDPE (P> 0.05). Compared to the control group, fish fed the 8% HDPE diet had significantly decreased levels of protein and ash (P < 0.05). In response to the increasing levels of HDPE exposure, the hepatosomatic index value, hepatocyte size, and liver glycogen level were increased, but lipid content was reduced in the liver tissues. Compared to the control treatment, fish fed the 8% HDPE diet had significant accumulations of total bile acids and different metabolism pathways such as bile acid biosynthesis, pyruvate metabolism, and carnitine synthesis. Significant enterocyte necrosis was documented in the foregut of fish fed the 2% or 8% HDPE diet; and significant cell sloughing was observed in the midgut and hindgut of fish fed the 8% HDPE diet. Fish fed the 2% HDPE diet harbored different microbiota communities compared to the control fish. This study demonstrates that HDPE ranging from 100 to 125 mu m in feed can be evacuated by yellow perch with no impact on growth. However, dietary exposure to HDPE decreased whole fish nutrition quality, altered nutrient metabolism and the intestinal histopathology as well as microbiota community of yellow perch. The results indicate that extended exposure may pose a risk to fish health and jeopardize the nutrition quality of aquacultured end product. This hypothesis remains to be investigated further. (C) 2022 Chinese Association of Animal Science and Veterinary Medicine. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co. Ltd.
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