Generation of human embryonic stem cell-derived lung organoids

Article Cell & Tissue Engineering

ApoE-Isoform-Dependent SARS-CoV-2 Neurotropism and Cellular Response

Cheng Wang et al.

Summary: The study suggests a potential causal role of ApoE4 in the severity of COVID-19, as tested in human-induced pluripotent stem cell (hiPSC) models for SARS-CoV-2 neurotropism. Increased infection rates of SARS-CoV-2 in ApoE4/4 neurons and astrocytes were observed, with changes in ApoE4 astrocytes upon infection. Remdesivir treatment was effective in inhibiting SARS-CoV2 infection in hiPSC neurons and astrocytes, highlighting the impact of risk factors on COVID-19 severity.

CELL STEM CELL (2021)

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Article Cell Biology

Human pluripotent stem-cell-derived alveolar organoids for modeling pulmonary fibrosis and drug testing

Jung-Hyun Kim et al.

Summary: The study developed alveolar organoids derived from human pluripotent stem cells to model pulmonary fibrosis and evaluate drug efficacy. Treatment with NP-011 effectively ameliorated fibrotic responses induced by TGF-beta 1 in the organoids through ERK signaling suppression, which also reversed bleomycin-induced lung fibrosis in mice. Additionally, NP-011 interacted with macrophages to accelerate collagen uptake and eliminate accumulated collagen in fibrotic lung tissues, suggesting its therapeutic potential for treating PF.

CELL DEATH DISCOVERY (2021)

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Article Cell & Tissue Engineering

SOX9 inactivation affects the proliferation and differentiation of human lung organoids

Lian Li et al.

Summary: The study utilized gene editing technology to inactivate the transcription factor SOX9 in human embryonic stem cells, revealing that SOX9 modulates the proliferative capacity of lung epithelium but is not an indispensable factor in the regulation of human lung epithelium development.

STEM CELL RESEARCH & THERAPY (2021)

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Article Chemistry, Multidisciplinary

Modeling Sporadic Alzheimer's Disease in Human Brain Organoids under Serum Exposure

Xianwei Chen et al.

Summary: This study established a human brain organoid-based sAD model using hiPSC-derived 3D brain organoids to mimic the serum exposure consequence of blood-brain barrier breakdown in AD patients. The model successfully recapitulated AD-like pathologies and could serve as a powerful platform for mechanistic study and therapeutic development in the future.

ADVANCED SCIENCE (2021)

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Article Biochemical Research Methods

Protocol for automated production of human stem cell derived liver spheres

Jose Meseguer-Ripolles et al.

Summary: This protocol describes the production of human liver spheres from pluripotent stem cell-derived hepatic progenitors, endothelial cells, and hepatic stellate cells, with up to 73 spheres per well generated through self-assembly in microwells. Automation using liquid handling and pipetting systems reduced sphere variability and provided a powerful tool for disease modeling and drug screening. More details can be found in Lucendo-Villarin et al. (2020) (https://doi.org/10.1088/1758-5090/abbdb2).

STAR PROTOCOLS (2021)

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Review Biology