Journal
STAR PROTOCOLS
Volume 3, Issue 2, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2022.101444
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Funding
- Fondation ARC pour la Recherche sur le Cancer [SL220110603471, PGA 120150202411, PGA1 RF20180206911]
- Institut National du Cancer [INCa_2013-098, INCa_2017-155]
- Agence Nationale de la Recherche [ANR-12-BSV1-0006-01]
- IDEX UNITI
- Laboratoire d'Excellence Toulouse Cancer (LABEX TOUCAN)
- LABEX TOUCAN
- Fondation ARC [DOC20170505820]
- French Ministry of Research
- Fondation pour la Recherche Medicale [FRM FDT20160435636, ECO201806006827, ECO202006011469, FDT202106012889]
- Agence Nationale de la Recherche (ANR) [ANR-12-BSV1-0006] Funding Source: Agence Nationale de la Recherche (ANR)
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This article presents a protocol for flow cytometry analysis of endothelial cells (ECs) and CD8+ T cells in murine tumor models, including baseline and post-immunotherapy analysis. The protocol is valuable for studying rare subsets of cells that play critical roles in antitumor immunity.
Here, we present a protocol for flow cytometry analysis of endothelial cells (ECs) and CD8+ T cells in murine tumor models, at baseline and after cancer immuno-therapy with anti-PD-1/anti-CTLA-4 antibodies. We provide gating strategies for identification of specific cell subsets including ECs from tumor-associated high endothelial venules (TA-HEVs), stem-like, and terminally exhausted CD8+ T cells. This protocol represents a valuable tool for the analysis of rare subsets of tumor ECs and CD8+ T cells with critical roles in antitumor immunity.For complete details on the use and execution of this protocol, please refer to Asrir et al. (2022).
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