Journal
STRUCTURAL DYNAMICS-US
Volume 4, Issue 4, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.4972069
Keywords
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Funding
- NSF-STC BioXFEL [STC-1231306]
- NIH [GM098248, GM109456, GM095583, GM097463]
- National Science Foundation Graduate Research Fellowship [1450681]
- U.S. Department of Energy, Office of Science, Basic Energy Sciences [DE-SC0002164]
- U.S. National Science Foundation [STC 1231306, 1551489]
- U.S. Department of Energy, Office of Science, Office of Basic Energy Sciences [DE-AC02-76SF00515]
- U.S. Department of Energy by Lawrence Livermore National Laboratory [DE-AC52-07NA27344]
- LLNL Lab-Directed Research and Development [012-ERD-031]
- National Institutes of Health [P41GM103393]
- Biodesign Center for Applied Structural Discovery at ASU
- Helmholtz Association Virtual Institute Dynamic Pathways
- BMBF [05K13GUK, 05K14CHA]
- European Union [317079]
- Division of Computing and Communication Foundations
- Direct For Computer & Info Scie & Enginr [1551489] Funding Source: National Science Foundation
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Mix-and-inject serial crystallography (MISC) is a technique designed to image enzyme catalyzed reactions in which small protein crystals are mixed with a substrate just prior to being probed by an X-ray pulse. This approach offers several advantages over flow cell studies. It provides (i) room temperature structures at near atomic resolution, (ii) time resolution ranging from microseconds to seconds, and (iii) convenient reaction initiation. It outruns radiation damage by using femtosecond X-ray pulses allowing damage and chemistry to be separated. Here, we demonstrate that MISC is feasible at an X-ray free electron laser by studying the reaction of M. tuberculosis A-lactamase microcrystals with ceftriaxone antibiotic solution. Electron density maps of the apo-beta-lactamase and of the ceftriaxone bound form were obtained at 2.8 angstrom and 2.4 angstrom resolution, respectively. These results pave the way to study cyclic and non-cyclic reactions and represent a new field of time-resolved structural dynamics for numerous substrate-triggered biological reactions. (C) 2016 Author(s).
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