Journal
RSC ADVANCES
Volume 12, Issue 27, Pages 17312-17320Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2ra02093a
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Funding
- National Natural Science Foundation of China [21765026]
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In this work, a portable U-disk electrochemical workstation combined with a screen-printed electrode is used for the quantitative determination of zearalenone (ZEN) using an aptamer sensor. The sensor demonstrated excellent sensitivity, selectivity, and reproducibility, with a wide linear range and a low detection limit. The proposed method offers a rapid, inexpensive, and real-time detection strategy for ZEN.
In this work, an aptasensor based on a portable U-disk electrochemical workstation in combination with a screen-printed electrode (SPE) is demonstrated for the quantitative determination of zearalenone (ZEN). The aptamer is immobilized on Au NPs@Ce-TpBpy COF (Covalent organic frameworks), which is modified on the surface of glassy carbon electrode. ZEN specifically binds to ZEN aptamer, which hinders the electron transfer and decreases the catalytic current of Au NPs@Ce-TpBpy COF for the reduction of hydrogen peroxide, measured by chronoamperometry (i-t). The quantitative detection of ZEN toxin is realized by a decrease of the catalytic current (Delta I). Under the optimal experimental conditions, the aptamer sensor exhibited excellent sensitivity, selectivity, reproducibility. A wide linear range of 1 pg mL(-1)-10.0 ng mL(-1) with a detection limit of 0.389 pg mL(-1) (at 3 sigma) was obtained. The linear equation is Delta I = 0.401 lg c + 1.948 with a correlation coefficient of 0.9906. The recovery is in the range of 93.0-104.7% for the cornflour samples. The proposed method offers a new strategy for the rapid, inexpensive, and real-time detection of ZEN.
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