Journal
SPRINGERPLUS
Volume 5, Issue -, Pages -Publisher
SPRINGER INTERNATIONAL PUBLISHING AG
DOI: 10.1186/s40064-016-2548-z
Keywords
Chinese white wax scale insect; Developmental stages; Reference gene; Temperature treatments; Tissues
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Funding
- Applied Basic Research Foundation of Yunnan Province [2013FA052, 2010ZC235]
- National High Technology Research and Development Program (863 Program) of China [2014AA021801]
- Special Fund for Forestry Research in the Public Interest [201504302, 201304808, 201204602]
- National Natural Science Foundation of China [31572337, 31000983]
- RIRI-CAF National Nonprofit Institute Research Grant [riricaf200904 M-3, riricaf2011006 M]
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Background: The Chinese white wax scale insect, Ericerus pela, is a well-known resource insect. The females and males are dramatically distinct at each developmental stage. We sought to identify suitable reference genes to use as internal controls in molecular research on E. plea. Results: geNorm, RefFinder and Normfinder analyses showed that beta Tub-2 was the best reference gene throughout different developmental stages; SdhA-1 was the most stable reference gene in different tissues, and beta Tub-1 was the most reliable reference gene under treatment with different temperatures. The results also showed that the optimal number of reference genes for analyzing target gene expression levels in the three experimental conditions was two. Conclusions: The identified reference genes are suitable reference genes for normalization in RT-qPCR of E. pela samples.
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