Journal
BIOMATERIALS ADVANCES
Volume 136, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bioadv.2022.212757
Keywords
UiO-66; Wet spinning; Chitosan scaffold; Osteogenic differentiation; Fosfomycin carrier; Osteomyelitis
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Funding
- Scientific and Technological Research Council of Turkey (TUBITAK) [218M324]
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This study evaluated the double function of UiO-66 MOF structure as a carrier for fosfomycin antibiotic and as an osteogenic differentiation promoter in 3D chitosan scaffolds. The results showed that CHI/UiO-66/FOS scaffolds had a good fiber structure and mechanical strength, and exhibited bactericidal activity against Staphylococcus aureus. Additionally, the scaffolds were biocompatible to pre-osteoblast cells and facilitated gene expression and extracellular matrix mineralization.
Metal-organic frameworks (MOFs) have recently emerged as a useful class of nanostructures with well-suited characteristics for drug delivery applications, due to the high surface area and pore size for efficient loading. Despite their use as a nano-carrier for controlled delivery of various types of drugs, the inherent osteo-conductive properties have stolen a great attention as a growing area of investigation. Here, we evaluated the double function of UiO-66 MOF structure as a carrier for fosfomycin antibiotic and also as an osteogenic differentiation promoter when introduced in 3D chitosan scaffolds, for the first time. Our results revealed that the wet-spun chitosan scaffolds containing fosfomycin loaded UiO-66 nanocrystals (CHI/UiO-66/FOS) possessed fiber mesh structure with integrated micro-scale fibers and increased mechanical strength. In vitro antibacterial studies indicated that CHI/UiO-66/FOS scaffolds showed bactericidal activity against Staphylococcus aureus. Moreover, the scaffolds were biocompatible to MC3T3-E1 pre-osteoblasts and significantly up-regulated the expression of osteogenesis-related genes and facilitated the extracellular matrix mineralization, in vitro. Taken together, our results demonstrate UiO-66 MOFs can present double functionality and CHI/UiO-66/FOS scaffolds hold a significant potential to be further explored as an alternative approach in treating infected bone defects like osteomyelitis.
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