Journal
SPRINGERPLUS
Volume 5, Issue -, Pages -Publisher
SPRINGER INTERNATIONAL PUBLISHING AG
DOI: 10.1186/s40064-016-2217-2
Keywords
Gamma-aminobutyric acid; Glutamate decarboxylase; Escherichia coli; Whole-cell biocatalyst; Bioconversion
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Funding
- National High Technology Research and Development Program of China [2015AA021005]
- Natural Science Foundation of Fujian Province [2014J01037]
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A simple and high efficient way for the synthesis of gamma-aminobutyric acid (GABA) was developed by using engineered Escherichia coli as whole-cell biocatalyst from L-glutamic acid (L-Glu). Codon optimization of Lactococcus lactis GadB showed the best performance on GABA production when middle copy-number plasmid was used as expression vector in E. coli BW25113. The highest production of GABA reached 308.96 g L-1 with 99.9 mol% conversion within 12 h, when E. coli Delta gabAB (pRB-lgadB) concentrated to an OD600 of 15 in 3 M L-Glu at 45 degrees C. Furthermore, the strain could be reused at least three cycles in 2 M crude L-Glu with an average productivity of 40.94 g L-1 h(-1). The total GABA yield reached 614.15 g L-1 with a molar yield over 99 %, which represented the highest GABA production ever reported. The whole-cell bioconversion system allowed us to achieve a promising cost-effective resource for GABA in industrial application.
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