Non-competitive fluorescence polarization immunosensing for CD9 detection using a peptide as a tracer

This paper is the first report of a non-competitive fluorescence polarization immunoassay (NC-FPIA) using a peptide as a tracer. The NC-FPIA can easily and quickly quantify the target after simply mixing them together. This feature is desirable for point-of-need applications such as clinical diagnostics, infectious disease screening, on-site analysis for food safety, etc. In this study, the NC-FPIA was applied to detect CD9, which is one of the exosome markers. We succeeded in detecting not only CD9 but also CD9 expressing exosomes derived from HeLa cells. This method can be applied to various targets if a tracer for the target can be prepared, and expectations are high for its future uses.

Automated summary

This paper presents the first report on a non-competitive fluorescence polarization immunoassay (NC-FPIA) using a peptide as a tracer. The NC-FPIA enables easy and quick quantification of the target by simple mixing. This method has potential applications in clinical diagnostics, infectious disease screening, on-site food safety analysis, etc. The study successfully applied the NC-FPIA to detect CD9 and CD9 expressing exosomes from HeLa cells, and it holds promise for future application to various targets.