4.5 Article

Deficiency of leukocyte-specific protein 1 (LSP1) alleviates asthmatic inflammation in a mouse model

Journal

RESPIRATORY RESEARCH
Volume 23, Issue 1, Pages -

Publisher

BMC
DOI: 10.1186/s12931-022-02078-7

Keywords

Macrophages; Leukocytes; LSP1; Asthma model

Funding

  1. Natural Science and Engineering Research Council (NSERC)
  2. Canadian Institutes for Health Research [MOP53167]
  3. Graduate Student Scholarship program from the Integrated Training Program in Infectious Disease, Food Safety and Public Policy (ITraP)
  4. Devolved Graduate Scholarship program from the Department of Veterinary Biomedical Sciences
  5. Graduate Student Scholarship program from the Western College of Veterinary Medicine, the University of Saskatchewan, Canada

Ask authors/readers for more resources

This study found that deficiency of LSP1 reduces airway hyper-responsiveness and lung inflammation in a mouse model of asthma. The levels of LSP1 were increased in the lungs of asthmatic mice and were associated with leukocyte recruitment. These findings suggest that LSP1 is involved in the pathogenesis of asthma.
Background Asthma is a major cause of morbidity and mortality in humans. The mechanisms of asthma are still not fully understood. Leukocyte-specific protein-1 (LSP-1) regulates neutrophil migration during acute lung inflammation. However, its role in asthma remains unknown. Methods An OVA-induced mouse asthma model in LSP1-deficient (Lsp1(-/-)) and wild-type (WT) 129/SvJ mice were used to test the hypothesis that the absence of LSP1 would inhibit airway hyperresponsiveness and lung inflammation. Results Light and electron microscopic immunocytochemistry and Western blotting showed that, compared with normal healthy lungs, the levels of LSP1 were increased in lungs of OVA-asthmatic mice. Compared to Lsp1(-/-) OVA mice, WT OVA mice had higher levels of leukocytes in broncho-alveolar lavage fluid and in the lung tissues (P < 0.05). The levels of OVA-specific IgE but not IgA and IgG1 in the serum of WT OVA mice was higher than that of Lsp1(-/-) OVA mice (P < 0.05). Deficiency of LSP1 significantly reduced the levels of IL-4, IL-5, IL-6, IL-13, and CXCL1 (P < 0.05) but not total proteins in broncho-alveolar lavage fluid in asthmatic mice. The airway hyper-responsiveness to methacholine in Lsp1(-/-) OVA mice was improved compared to WT OVA mice (P < 0.05). Histology revealed more inflammation (inflammatory cells, and airway and blood vessel wall thickening) in the lungs of WT OVA mice than in those of Lsp1(-/-) OVA mice. Finally, immunohistology showed localization of LSP1 protein in normal and asthmatic human lungs especially associated with the vascular endothelium and neutrophils. Conclusion These data show that LSP1 deficiency reduces airway hyper-responsiveness and lung inflammation, including leukocyte recruitment and cytokine expression, in a mouse model of asthma.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.5
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available