Journal
CURRENT ONCOLOGY
Volume 29, Issue 6, Pages 4274-4284Publisher
MDPI
DOI: 10.3390/curroncol29060342
Keywords
squamous cell head-neck cancer; radiotherapy; tumor-infiltrating lymphocytes; hypoxia; prognosis
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Microenvironmental conditions play a crucial role in controlling the entrance and thriving of cytotoxic lymphocytes in tumors. In this study, the density of tumor-infiltrating lymphocytes (TILs) in squamous cell head-neck tumors (HNSCC) was investigated, and the link between markers of hypoxia and TIL density was assessed. The results showed that high TIL density was associated with better survival outcomes, and overexpression of HIF1 alpha, HIF2 alpha, and CA9 was significantly linked with poor infiltration by TILs. These findings suggest that targeting HIF and CA9-related pathways in immunologically cold tumors may be a potential therapeutic intervention.
Microenvironmental conditions control the entrance and thriving of cytotoxic lymphocytes in tumors, allowing or preventing immune-mediated cancer cell death. We investigated the role of tumor-infiltrating lymphocyte (TIL) density in the outcome of radiotherapy in a series of squamous cell head-neck tumors (HNSCC). Moreover, we assessed the link between markers of hypoxia and TIL density. One-hundred twenty-one patients with HNSCC treated prospectively with radical radiotherapy/chemo-radiotherapy were analyzed. The assessment of TIL density was performed on hematoxylin and eosin biopsy sections before radiotherapy. TIL density ranged from 0.8 to 150 lymphocytes per x40 optical field (median 27.5). Using the median value, patients were grouped into two categories of low and high TIL density. Early T-stage tumors had a significantly higher TIL density (p < 0.003), but we found no association with N-stage. Overexpression of HIF1 alpha, HIF2 alpha, and CA9 was significantly linked with poor infiltration by TILs (p < 0.03). A significant association of high TIL density with better disease-specific overall survival and improved locoregional relapse-free survival was noted (p = 0.008 and 0.02, respectively), which was also confirmed in multivariate analysis. It is concluded that HNSCC phenotypes that allow for the intratumoral accumulation of lymphocytes have a better outcome following radical radiotherapy/chemo-radiotherapy. Intratumoral-activated HIF- and CA9-related pathways characterize immunologically cold tumors and may be used as targets for therapeutic interventions.
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