A label-free and self-circulated fluorescent biosensor for sensitive detection of ten-eleven translocation 1 in cancer cells

We develop for the first time a label-free and self-circulated fluorescent biosensor for sensitive detection of ten-eleven translocation 1 (TET1) activity in cancer cells. This biosensor displays good specificity and high sensitivity without the need for any antibodies/exogenous primers and it can accurately quantify TET1 activity at the single-cell level. Moreover, it can be employed for the measurement of kinetic parameters and screening of inhibitors.

Automated summary

A label-free and self-circulated fluorescent biosensor has been developed for the sensitive detection of TET1 activity in cancer cells. This biosensor demonstrates good specificity and high sensitivity without the need for antibodies or exogenous primers, enabling accurate quantification of TET1 activity at the single-cell level. Additionally, it can be used for measuring kinetic parameters and screening inhibitors.