4.8 Article

Guidelines for measuring reactive oxygen species and oxidative damage in cells and in vivo

Journal

NATURE METABOLISM
Volume 4, Issue 6, Pages 651-662

Publisher

NATURE PORTFOLIO
DOI: 10.1038/s42255-022-00591-z

Keywords

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Funding

  1. Medical Research Council UK [MC_UU_00015/3]
  2. Wellcome Trust Investigator award [220257/Z/20/Z]
  3. National Institutes of Health (NIH), USA [AI145406, CA165065, CA266342, HL114453, AI156924, NS076511, AI156923, NS061817, NS117000]
  4. National Institute of Environmental Health Sciences of the US NIH [ES003598]
  5. National Institute on Aging of the US NIH [AG052374]
  6. Universidad de la Republica, Uruguay [CSIC_2018, EI_2020]
  7. National Medical Research Council of Singapore
  8. National University of Singapore
  9. National Research Foundation, Singapore
  10. Tan Chin Tuan Foundation
  11. NIH [GM 79465, GM 139245, ES 28096]
  12. JST CREST [JPMJCR19H4]
  13. JSPS Kakenhi [JP16H06276, JP19H05462, JP20H05502]
  14. Research Grant of the Princess Takamatsu Cancer Research Fund [19-25126]
  15. German Research Council (DFG) [TRR184, SPP2306]
  16. European Research Council [742039, 2016-741366]
  17. National Natural Science Foundation of China [32030053, 32150710522]
  18. Novo Nordisk Foundation [NNF13OC0004294, NNF19OC0058493, NNF20SA0064214]
  19. Swedish Research Council [2015-00418]
  20. Swedish Cancer Foundation
  21. Knut and Alice Wallenberg foundation
  22. Novo Nordisk Foundation
  23. European Research Council (ERC) [742039] Funding Source: European Research Council (ERC)
  24. Wellcome Trust [220257/Z/20/Z] Funding Source: Wellcome Trust

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This article discusses the important roles of reactive oxygen species (ROS) in health and disease, and proposes guidelines and best practices for the nomenclature and assessment of ROS, oxidative reactions, and oxidative damage in cells, tissues, and in vivo.
Multiple roles of reactive oxygen species (ROS) and their consequences for health and disease are emerging throughout biological sciences. This development has led researchers unfamiliar with the complexities of ROS and their reactions to employ commercial kits and probes to measure ROS and oxidative damage inappropriately, treating ROS (a generic abbreviation) as if it were a discrete molecular entity. Unfortunately, the application and interpretation of these measurements are fraught with challenges and limitations. This can lead to misleading claims entering the literature and impeding progress, despite a well-established body of knowledge on how best to assess individual ROS, their reactions, role as signalling molecules and the oxidative damage that they can cause. In this consensus statement we illuminate problems that can arise with many commonly used approaches for measurement of ROS and oxidative damage, and propose guidelines for best practice. We hope that these strategies will be useful to those who find their research requiring assessment of ROS, oxidative damage and redox signalling in cells and in vivo. Reactive oxygen species (ROS) have important roles in health and disease, but are chemically complex and difficult to measure accurately. This consensus statement proposes guidelines and best practices on the nomenclature and assessment of ROS, oxidative reactions and oxidative damage in cells, tissues and in vivo.

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