Journal
RSC ADVANCES
Volume 12, Issue 30, Pages 19040-19053Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d2ra02870k
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- Faculty of Pharmacy at King Saud University (KSU), Riyadh, Saudi Arabia
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Amino acids can form complexes with poorly water-soluble drugs, improving their physicochemical properties and dissolution profile through molecular interactions. Salt formation between acidic drugs and basic amino acids is the main contributor to solubility enhancement. This study found that non-ionic interactions also play a role in solubility improvement, independent of pH.
Amino acids have shown promising abilities to form complexes with poorly water-soluble drugs and improve their physicochemical properties for a better dissolution profile through molecular interactions. Salt formation via ionization between acidic drugs and basic amino acids is known as the major contributor to solubility enhancement. However, the mechanism of solubility enhancement due to non-ionic interactions, which is less pH-dependent, remains unclear. The aim of this study is to evaluate non-ionic interactions between a model acidic drug, indomethacin (IND), and basic amino acids, arginine, lysine and histidine, in water. At low concentrations of amino acids, IND-arginine and IND-lysine complexes have shown a linear relationship (A(L)-type phase solubility diagram) between IND solubility and amino acid concentration, producing similar to 1 : 1 stoichiometry of drug-amino acid complexes as expected due to the strong electrostatic interactions. However, IND-histidine complexes have shown a nonlinear relationship with lower improvement in IND solubility due to the weaker electrostatic interactions when compared to arginine and lysine. Interestingly, the results have also shown that at high arginine concentrations, the linearity was lost between IND solubility and amino acid concentration with a negative diversion from linearity, following the type-A(N) phase solubility. This is indicative that the electrostatic interaction is being interrupted by non-electrostatic interactions, as seen with histidine. The IND-lysine complex, on the other hand, showed a complex curvature phase solubility diagram (type B-S) as lysine self-assembles and polymerizes at higher concentrations. The freeze-dried drug-amino acid solids were further characterized using thermal analysis and infrared spectroscopy, with results showing the involvement of weak non-ionic interactions. This study shows that the solubility improvement of an insoluble drug in the presence of basic amino acids was due to both non-ionic and ionic interactions.
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