Journal
PHYTOPATHOLOGY RESEARCH
Volume 4, Issue 1, Pages -Publisher
SPRINGERNATURE
DOI: 10.1186/s42483-022-00130-4
Keywords
Botrytis cinerea; Peroxisome; Lipid metabolism; Infection; Environmental stress
Categories
Funding
- Shandong Provincial Natural Science Foundation [ZR2020KC003]
- Key Research and Development Program of Shandong Province [2019YQ017]
- Shandong Province `Double-Hundred Talent Plan' [WST2018008]
- Taishan Scholar Construction Foundation of Shandong Province [TSHW20130963]
- Scientific Research Fund for High-Level Talents in Qingdao Agricultural University [20210036]
Ask authors/readers for more resources
This study reveals that activation of the peroxisome pathway contributes to the initial infection and ability to cope with environmental stress in Botrytis cinerea. They found that the overexpression of genes involved in the peroxisome pathway was upregulated in response to the presence of tomato leaves. The increase in peroxisome number and lipid metabolism was associated with infection-related structures and the ability to penetrate plant cells.
Peroxisomes are very important organelles in eukaryotic cells and participate in various biological processes, including pathogen-host interactions. A variety of proteins involved in peroxisome proliferation and metabolic activity within peroxisomes have been shown to be essential for full virulence of several phytopathogenic fungi. However, the effects of changes in the number of peroxisomes and proteins involved in the peroxisome pathway on the pathogenicity of Botrytis cinerea have rarely been reported. In this study, by analysing transcriptome data and RT-qPCR validation, we found that more than half of the genes annotated to the peroxisome pathway in B. cinerea were upregulated more than twofold between mycelial samples cultured in medium with tomato leaves and without tomato leaves. A strain of B. cinerea with fluorescently labelled peroxisomes was obtained by overexpression of GFP fused to peroxisomal targeting signal 1 (the tripeptide 'SKL'). The addition of tomato leaves to the liquid medium induced a significant increase in the number of peroxisomes, beta-oxidation level, H2O2 content, and acetyl-CoA level in B. cinerea mycelia. When B. cinerea was cultured with oleic acid as the sole carbon source, the formation of infection-related structures and their penetration into plant cells were found to be associated with peroxisome pathway activity. Furthermore, peroxisome proliferation and lipid metabolism increased in response to different extracellular stresses in B. cinerea. Taken together, our results confirmed that activation of the peroxisome pathway in B. cinerea contributes to the initial infection and the ability to cope with environmental stress.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available