Journal
FOODS
Volume 11, Issue 13, Pages -Publisher
MDPI
DOI: 10.3390/foods11131843
Keywords
polystyrene-polyacrylonitrile nanofibers; quinolones; packed-fiber solid-phase extraction; HPLC-MS/MS; pure liquid milk
Categories
Funding
- Key Research and Development Projects of Anhui Province [202104f06020007]
- Key Projects of Anhui Provincial Department of Education [KJ2021A0888]
- Key Projects of Anhui Science and Technology University [2021zrzd12]
- Anhui Provincial Major Science and Technology Project [18030701212]
- Talent Foundation of Anhui Science and Technology University [SPYJ 202001]
- College Students' Innovative Entrepreneurial Training Plan Program [202110879050]
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In this study, a sensitive and cost-effective method combining packed-fiber solid-phase extraction and high-performance liquid chromatography coupled with tandem mass spectrometry was developed for simultaneous detection of nine quinolones in pure milk. The method showed high accuracy, good linearity, and low method quantification limits.
In this study, a packed-fiber solid-phase extraction (PFSPE)-based method was developed to simultaneously detect nine quinolones, including enrofloxacin (ENR), ciprofloxacin (CIP), ofloxacin (OFL), pefloxacin (PEF), lomefloxacin (LOM), norfloxacin (NOR), sarafloxacin (SAR), danofloxacin (DAN), and difloxacin (DIF), in pure milk, using high-performance liquid chromatography coupled with tandem mass spectrometry (HPLC-MS/MS). Polystyrene (PS) and polyacrylonitrile (PAN) were combined to form PS-PAN composite nanofibers through electrospinning. The nanofibers were used to prepare the home-made extraction columns, and the process was optimized and validated using blank pure milk. The analytical method showed high accuracy, and the recoveries were 88.68-97.63%. Intra-day and inter-day relative standard deviations were in the ranges of 1.11-6.77% and 2.26-7.17%, respectively. In addition, the developed method showed good linearity (R-2 >= 0.995) and low method quantification limits for the nine quinolones (between 1.0-100 ng/mL) for all samples studied. The nine quinolones in the complex matrix were directly extracted using 4.0 mg of PS-PAN composite nanofibers as a sorbent and completely eluted in 100 mu L elution solvent. Therefore, the developed PFSPE-HPLC-MS/MS is a sensitive and cost-effective technique that can effectively detect and control nine quinolones in dairy products.
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