Journal
JOURNAL OF TOXICOLOGIC PATHOLOGY
Volume 35, Issue 3, Pages 225-235Publisher
JAPANESE SOC TOXICOLOGIC PATHOLOGY
DOI: 10.1293/tox.2022-0017
Keywords
chemical hepatotoxicity; in vitro; organoids; rat; toxicologic pathologist
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Funding
- Ministry of Education, Culture, Sports, Science [20H03146]
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The development of in vitro toxicity assessment methods using cultured cells has gained popularity for promoting animal welfare in animal experiments. This article briefly discusses the current status of hepatoxicity assessment using human and rat-derived hepatocytes, focusing on the liver organoid method. It also proposes how toxicologic pathologists should assess toxicity regarding the distribution of undifferentiated and differentiated cells in the organoid.
The development of in vitro toxicity assessment methods using cultured cells has gained popularity for promoting animal welfare in animal experiments. Herein, we briefly discuss the current status of hepatoxicity assessment using human-and rat-derived hepatocytes; we focus on the liver organoid method, which has been extensively studied in recent years, and discuss how toxicologic pathologists can use their knowledge and experience to contribute to the development of in vitro chemical hepatotoxicity assessment methods for drugs, pesticides, and chemicals. We also propose how toxicological pathologists should assess toxicity regarding the putative distribution of undifferentiated and differentiated cells in the organoid when liver organoids are observed in hematoxylin and eosin-stained specimens. This was done while considering the usefulness and limitations of in vitro studies for toxicologic pathology assessment.
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