Journal
ACS APPLIED BIO MATERIALS
Volume 5, Issue 7, Pages 3134-3145Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acsabm.2c00081
Keywords
zinc gallate; PLNPs; B-1(0)(OH)(3); poly(vicinal-dioal); BNCT; photon-imaging; fibrosarcoma
Funding
- University Grants Commission (UGC) , New Delhi [BAEC/12/2020, BAEC/27/2021]
Ask authors/readers for more resources
Based on boron neutron capture therapy (BNCT), researchers have developed a zinc gallate-based nanoformulation for cancer treatment and diagnosis. The nanoformulation, with its functionalized surface, effectively targets tumor cells and demonstrates excellent therapeutic efficacy in in vitro and in vivo studies.
On the basis of the boron neutron capture therapy (BNCT) modality, we have designed and synthesized a zinc gallate (ZnGa2O4)-based nanoformulation for developing an innovative theranostic approach for cancer treatment. Initially, the (ZnGa1.995Cr0.005O4 or ZnGa2O4:(0.5%)Cr persistent luminescence nanopartides (PLNPs) embedded on silica matrix were synthesized. Their surface functionalization was performed using organic synthesis strategies to attach the amine functional moieties which were further coupled with poly(vicinal diol). These diols were helpful for conjugation with B-1(0)(OH)(3), which subsequently served to couple with an in-house-synthesized variant of pH-(low)-insertion peptide (pHLIP) finally giving a tumor-targeting nanoformulation. Most importantly, the polymeric diols helped in conjugation of a substantial number of B-10 to provide the therapeutic dose required for effective BNCT. This nanoformulation internalized substantially (similar to 80%) to WEHI-164 cancer cells within 6 h. Tumor homing studies indicated that the accumulation of this formulation at the acidic tumor site was within 2 h. The in vitro evaluation of the formulation against WEHI-164 cancer cells followed by neutron irradiation revealed its potent cytotoxicity with IC50 similar to 25 mu M. In the case of studies on animal models, the melanoma-induced CS7BL/6 and fibrosarcoma-induced BALB/c mice were treated with formulations through intratumoral and intravenous injections, respectively, followed by neutron irradiation, leading to a significant killing of the cancer cells, which was evidenced by a reduction in tumor volume (75-80%) as compared with a control tumor. Furthermore, the histopathological studies confirmed a damaging effect only on tumor cells, while there was no sign of damage to the vital organs in treated mice as well as in controls.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available