☆ 4.8 Review

Is enzyme immobilization a mature discipline? Some critical considerations to capitalize on the benefits of immobilization

CHEMICAL SOCIETY REVIEWS (2022)

Related references

Note: Only part of the references are listed.
Article Nanoscience & Nanotechnology

Selective Coimmobilization of His-Tagged Enzymes on Yttrium-Stabilized Zirconia-Based Membranes for Continuous Asymmetric Bioreductions

Daniel Andres-Sanz et al.

Summary: In this study, a novel interaction between YSZ materials and His-tagged enzymes was discovered, enabling the fabrication of multifunctional biocatalytic membranes for continuous biomanufacturing. This system succeeded in in situ redox cofactor recycling, maintaining specific productivity with minimal NADH usage, and accumulating a high enzyme turnover number in a 24-hour period.

ACS APPLIED MATERIALS & INTERFACES (2022)

Add to Collection
Review Biochemistry & Molecular Biology

Combining technology with liquid-formulated lipases for in-spec biodiesel production

Chinmayi Bhatt et al.

Summary: Enzymatic biodiesel production offers significant environmental advantages and has the potential to be as effective as conventional chemically catalyzed biodiesel production. However, its widespread industrial adoption has been restricted due to higher capital cost, longer reaction time, and sensitivity of enzyme processes. Additionally, the lack of research on bringing biodiesel product into final specification has undermined industrial confidence. Further research on liquid lipase formulations could lead to improved enzymatic biodiesel production.

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2022)

Add to Collection
Article Biotechnology & Applied Microbiology

Preparation of combined cross-linked enzyme aggregates containing galactitol dehydrogenase and NADH oxidase for l-tagatose synthesis via in situ cofactor regeneration

Xue-Yong Li et al.

Summary: The combi-CLEAs, consisting of Gdh and Nox, showed an activity of 210 U g(-1) under optimized preparation conditions. They exhibited higher thermostability and retained 51.5% of their original activity after eight cycles of reuse. The combi-CLEAs were successfully used for the synthesis of l-tagatose without any by-products, showcasing their potential for industrial bioconversion of galactitol.

BIOPROCESS AND BIOSYSTEMS ENGINEERING (2022)

Add to Collection
Article Chemistry, Physical

Biocatalytically Active and Stable Cross-Linked Enzyme Crystals of Halohydrin Dehalogenase HheG by Protein Engineering

Marcel Staar et al.

Summary: The researchers achieved immobilization of halohydrin dehalogenase HheG through the introduction of a cysteine residue, improving its stability and activity. The cross-linked enzyme crystals displayed better performance than the soluble enzyme, showing improved stability and activity at higher temperatures, lower pH values, and in the presence of water-miscible organic solvents.

CHEMCATCHEM (2022)

Add to Collection
Review Chemistry, Multidisciplinary

Nanoflowers: A New Approach of Enzyme Immobilization

Felipe Pereira da Costa et al.

Summary: Enzymes are biocatalysts known for their versatility, selectivity, and favorable operating conditions compared to chemical catalysts. However, their large-scale application is limited by high costs and low stability under extreme reaction conditions. Immobilization techniques can solve these problems, and organic-inorganic hybrid nanoflowers have shown advantages such as high surface area, simplicity of synthesis, high stability, and catalytic activity in enzymatic catalysis.

CHEMICAL RECORD (2022)

Add to Collection
Article Biophysics

Enhancing stability and by-product tolerance of β-glucuronidase based on magnetic cross-linked enzyme aggregates

Bin Wei et al.

Summary: In this study, magnetic cross-linking beta-glucuronidase aggregates (MCLEAs) were constructed for the production of glycyrrhetinic acid (GA). The results showed that MCLEAs enhanced the activity, stability, and glucuronic acid tolerance of the enzymes. This provided an efficient strategy for enzyme immobilization with significant industrial applications.

COLLOIDS AND SURFACES B-BIOINTERFACES (2022)

Add to Collection
Review Biochemical Research Methods

Microenvironmental effects can masquerade as substrate channelling in cascade biocatalysis

Walaa Abdallah et al.

Summary: Natural cascades often use spatial organization to introduce beneficial substrate channeling mechanisms, which has been widely adopted in engineered multienzyme cascades. Utilizing new molecular scaffolds now allows testing the effects of spatial organization on cascade kinetics, but these scaffolds can also change the microenvironment experienced by the assembled enzymes, complicating kinetic analysis.

CURRENT OPINION IN BIOTECHNOLOGY (2022)

Add to Collection
Article Biochemistry & Molecular Biology

The combination of covalent and ionic exchange immobilizations enables the coimmobilization on vinyl sulfone activated supports and the reuse of the most stable immobilized enzyme

Sara Arana-Pena et al.

Summary: This study achieved the coimmobilization of lipases from Rhizomucor miehei and Candida antarctica, successfully immobilizing both enzymes even when one of them cannot be immobilized initially. A special strategy was developed to desorb inactivated enzyme and re-immobilize a fresh sample, allowing for enzyme reuse.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2022)

Add to Collection
Review Chemistry, Organic

Enantioselective desymmetrization reactions in asymmetric catalysis

Carmen Najera et al.

Summary: This review summarizes the recent advancements in enantioselective desymmetrization reactions using metal-catalyzed, organocatalyzed, and enzymatic processes. These asymmetric strategies have been successfully applied to a wide range of organic compounds, including diarylalkanes, silanes, alcohols, amines, and unsaturated compounds. The classical desymmetrization of various compounds by acylation, alkylation, and oxidation methods are important processes in organic synthesis. Desymmetrization methodologies have also been applied to small ring carbocycles and heterocyclic systems. The synthetic applications of these strategies in the total synthesis of natural products and biologically active compounds are highlighted.

TETRAHEDRON (2022)

Add to Collection
Editorial Material Chemistry, Physical

Mass-based biocatalyst metrics to guide protein engineering and bioprocess development

Murray P. Meissner et al.

Summary: Metrics are valuable for evaluating biocatalyst performance and setting goals for enzyme and bioprocess research and development. However, there are challenges to overcome for wider acceptance of this approach in industry and academia.

NATURE CATALYSIS (2022)

Add to Collection
Article Environmental Sciences

Insolubilization of Tramates versicolor laccase as cross-linked enzyme aggregates for the remediation of trace organic contaminants from municipal wastewater

Jenet George et al.

Summary: The novelty of this study lies in the biocatalytic treatment of trace organic contaminants in municipal wastewater using insolubilized laccase. The developed cross-linked enzyme aggregates (CLEAs) showed higher efficiency and resistance to inhibition compared to free laccase. The CLEAs laccase demonstrated excellent removal performance in batch and perfusion basket reactor systems.

ENVIRONMENTAL RESEARCH (2022)

Add to Collection
Article Chemistry, Multidisciplinary

Intraparticle Macromolecular Migration Alters the Structure and Function of Proteins Reversibly Immobilized on Porous Microbeads

Eleftheria Diamanti et al.

Summary: This study investigates the passive migration of proteins during sample storage and its impact on protein functionality. The migration of immobilized proteins on porous supports is assessed, revealing differences in migration patterns between different proteins. Additionally, the effect of macromolecular migration on the functional and structural properties of bound proteins and enzymes is explored.

ADVANCED MATERIALS INTERFACES (2022)

Add to Collection
Article Chemistry, Applied

Enzymatic clarification of orange juice in continuous bed reactors: Fluidized-bed versus packed-bed reactor

Lucas Dal Magro et al.

Summary: This study immobilized a commercial enzyme cocktail for fruit juice clarification on chitosan beads and found that despite low activity recovery after immobilization, the stability of the immobilized enzyme was greater than that of the free enzyme. The enzyme retained higher activity levels under extreme conditions, and the fluidized-bed reactor showed better juice clarification capacity compared to the packed-bed reactor.

CATALYSIS TODAY (2021)

Add to Collection
Article Chemistry, Applied

Immobilization of lipases via interfacial activation on hydrophobic supports: Production of biocatalysts libraries by altering the immobilization conditions

Sara Arana-Pena et al.

Summary: CALB and CRL showed similar properties under different immobilization conditions, while RML displayed significant changes. The effects of immobilization conditions on substrate activity and stability vary greatly, requiring careful selection based on specific circumstances.

CATALYSIS TODAY (2021)

Add to Collection
Review Chemistry, Multidisciplinary

Biocatalysis: Enzymatic Synthesis for Industrial Applications

Shuke Wu et al.

Summary: Biocatalysis is widely used as an alternative to chemical catalysis in various fields, with enzymes playing a key role in organic synthesis, particularly in the production of chiral compounds for pharmaceuticals and flavors. Biocatalysts are also utilized on a large scale for the production of specialty and bulk chemicals. This review focuses on scalable chemical production using enzymes, discussing opportunities, limitations, and emerging enzyme classes through illustrative examples.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2021)

Add to Collection
Review Engineering, Biomedical

Crosslinking strategies for silk fibroin hydrogels: promising biomedical materials

Maryam Farokhi et al.

Summary: Silk fibroin hydrogels have strong biomimetic potential and are excellent candidates for tissue engineering due to their tunable properties. Different crosslinking strategies, either chemical or physical, can alter the structure of silk fibroin and improve its mechanical stability. However, each method has its own advantages and disadvantages, and the choice of method depends on the specific application requirements.

BIOMEDICAL MATERIALS (2021)

Add to Collection
Article Environmental Sciences

A study of external mass transfer effect on biodegradation of phenol using low-density polyethylene immobilizedBacillus flexusGS1 IIT (BHU) in a packed bed bioreactor

Ganesh Swain et al.

Summary: The study investigated the impact of external mass transport on the biodegradation rate of phenol in a packed bed bioreactor, and developed a new empirical correlation for phenol biodegradation in the PBBR. Bacillus flexus GS1 IIT (BHU) was identified as a potential bacterial species for phenol degradation in the PBBR.

WATER AND ENVIRONMENT JOURNAL (2021)

Add to Collection
Review Green & Sustainable Science & Technology

Closing the loop of cereal waste and residues with sustainable technologies: An overview of enzyme production via fungal solid-state fermentation

Dominika Alexa Teigiserova et al.

Summary: This study conducted a targeted review of enzyme production from cereal food waste and residues for potential utilization in the circular economy, identifying key substrates such as wheat, rice, and corn, along with enzymes like xylanase, CMCase, and FPase. The study found that xylanase from wheat bran, rice straw, and their combination showed great potential for reaching high enzyme activity and promising revenues, demonstrating the importance of scale-up experiments for industrial application. Improvements in transparency and knowledge sharing for cross-disciplinary research were also identified, including harmonized enzyme names and criteria for expressing enzyme activity per mass unit.

SUSTAINABLE PRODUCTION AND CONSUMPTION (2021)

Add to Collection
Article Green & Sustainable Science & Technology

Liquid lipase preparations designed for industrial production of biodiesel. Is it really an optimal solution?

Rodolpho R. C. Monteiro et al.

Summary: The study discusses the application of liquid lipase formulation Eversa in biodiesel production and the enhancement of its performance through immobilization, proposing the concept of using combi-lipases is preferable over the concept of optimal lipase for biodiesel production.

RENEWABLE ENERGY (2021)

Add to Collection
Article

Intensification of biokinetics of enzymes using ultrasound-assisted methods: a critical review

Altab Khan et al.

Biophysical Reviews (2021)

Add to Collection
Article Energy & Fuels

Biolubricant Production from Several Oleaginous Feedstocks Using Lipases as Catalysts: Current Scenario and Future Perspectives

Iara C. A. Bolina et al.

Summary: Biolubricants have gained more attention in recent years compared to conventional petroleum-based lubricants, due to their advantages in terms of biodegradability, low toxicity, excellent lubricating performance, and minimal environmental impact. They can be efficiently produced using various types of oleaginous feedstocks and different chemical reactions catalyzed by lipases, making them promising in the context of Green Chemistry. The review also highlights the potential for enzymatic biolubricant production on an industrial scale, as well as the challenges that need to be addressed for further development in this field.

BIOENERGY RESEARCH (2021)

Add to Collection
Review Biotechnology & Applied Microbiology

Advances in the application of deep eutectic solvents based aqueous biphasic systems: An up-to-date review

Emmanuel A. Oke et al.

Summary: The success of extracting biomolecules and bioactive compounds from natural matrix greatly relies on the chosen method, in which the use of ILs and DES in ABS has gained attention for their potential advantages. DES offer a new pathway for separation and purification in ABS, though there are limitations in terms of protein extraction efficiency.

BIOCHEMICAL ENGINEERING JOURNAL (2021)

Add to Collection
Article Agricultural Engineering

Eco-friendly production of trimethylolpropane triesters from refined and used soybean cooking oils using an immobilized low-cost lipase (Eversa>® Transform 2.0) as heterogeneous catalyst

Wagner C. A. Carvalho et al.

Summary: Trimethylolpropane triesters (TMPTEs) were produced through enzymatic hydroesterification of refined soybean oil (RSO) or used soybean cooking oil (USCO). Immobilized Eversa (R) Transform 2.0 showed the highest activity, retaining above 90% of its original activity after 13 consecutive reaction cycles. The produced TMPTEs exhibited good cold temperature properties with pour point values between -11 and -9 degrees C.

BIOMASS & BIOENERGY (2021)

Add to Collection
Review Biochemistry & Molecular Biology

Site-Directed Mutagenesis of Modular Polyketide Synthase Ketoreductase Domains for Altered Stereochemical Control

Erin E. Drufva et al.

Summary: Researchers have extensively studied ketoreductase domains in bacterial modular type I polyketide synthases with the goal of altering their stereoselectivity through site-directed mutagenesis, as they contribute to much of the stereochemical complexity in pharmaceutically active polyketide scaffolds. However, translating these findings to alter stereocontrol in the context of a complex multidomain enzymatic assembly line remains challenging.

CHEMBIOCHEM (2021)

Add to Collection
Article Chemistry, Physical

Understanding the Deactivation of Ag-ZrO2/SiO2 Catalysts for the Single-step Conversion of Ethanol to Butenes

Fan Lin et al.

Summary: Ag-ZrO2/SBA-16 has been found to efficiently catalyze the conversion of ethanol to butene, but it deactivates over time due to coke deposition, Ag particle sintering, and Ag oxidation state change.

CHEMCATCHEM (2021)

Add to Collection
Review Chemistry, Multidisciplinary

Metal-Organic Framework-Based Enzyme Biocomposites

Weibin Liang et al.

Summary: Enzymes play a crucial role in chemical synthesis and biotechnology, but their activity can be easily affected by external stressors. Metal-organic frameworks (MOFs) offer protection to enzymes through encapsulation or infiltration, allowing them to maintain activity under challenging conditions.

CHEMICAL REVIEWS (2021)

Add to Collection
Article Energy & Fuels

Evaluation of process integration for the intensification of a biotechnological process

Francesca Meyer et al.

Summary: The study analyzes the effects and potential for further process integration of integrating an extraction centrifuge for intermediate separation and pre-purification for product separation in a multi-enzyme cascade. Different process configurations are compared using a model-based approach to evaluate efficiency, identifying a significant increase in space-time yield within a certain operating window for the highly integrated process.

CHEMICAL ENGINEERING AND PROCESSING-PROCESS INTENSIFICATION (2021)

Add to Collection
Review Biochemistry & Molecular Biology

Flow Biocatalysis: A Challenging Alternative for the Synthesis of APIs and Natural Compounds

Micol Santi et al.

Summary: Biocatalysts have become a popular choice in the past few years, especially when applied in continuous flow systems, which are crucial for overcoming the limitations of traditional enzymatic catalysis and enabling more sustainable synthetic pathways.

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES (2021)

Add to Collection
Review Engineering, Biomedical

Plant based cross-linkers for tissue engineering applications

Abhishek Indurkar et al.

Summary: The utility of plant-based materials in tissue engineering has been increasing exponentially. Natural cross-linkers derived from plants offer several advantages over synthetic ones in scaffolds and hydrogels. Incorporating natural compounds like ferulic acid and genipin can promote cellular proliferation and growth, and provide anti-microbial, antioxidant, and anti-inflammatory effects, enhancing scaffold characteristics.

JOURNAL OF BIOMATERIALS APPLICATIONS (2021)

Add to Collection
Article Biochemistry & Molecular Biology

Immobilization of Eversa(R) Transform via CLEA Technology Converts It in a Suitable Biocatalyst for Biolubricant Production Using Waste Cooking Oil

Jose Renato Guimaraes et al.

Summary: The study evaluated the performance of Eversa-mCLEA in the enzymatic synthesis of biolubricants, showing good activity in transesterification reactions with different alcohols. By adjusting the WCO/alcohol molar ratio and enzyme load, high yields of bio-lubricants can be achieved.

MOLECULES (2021)

Add to Collection
Article Biochemistry & Molecular Biology

Environmental Assessment of Enzyme Production and Purification

Martin Becker et al.

Summary: The importance of bioprocesses has been increasing in recent decades, with a focus on sustainability. Research shows that the production and purification of enzymes play a significant role in sustainability assessment. It is essential to consider the contribution of enzyme synthesis and purification in evaluating the sustainability of bioprocesses.

MOLECULES (2021)

Add to Collection
Article Energy & Fuels

Enhanced activity and stability of Rhizomucor miehei lipase by mutating N-linked glycosylation site and its application in biodiesel production

Miao Tian et al.

Summary: A lipase mutant N59H, engineered with high activity, thermostability, and methanol resistance, showed excellent performance in biodiesel production, demonstrating great potential for commercial application.

FUEL (2021)

Add to Collection
Article Biochemistry & Molecular Biology

Enzyme-support interactions and inactivation conditions determine Thermomyces lanuginosus lipase inactivation pathways: Functional and florescence studies

Priscila M. Paiva Souza et al.

Summary: The study immobilized lipase on agarose and treated the enzyme with different blocking agents. It was found that enzyme specificity changed significantly during inactivation, influenced by immobilization and inactivation conditions.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

Add to Collection
Review Engineering, Chemical

Multi-Enzyme Systems in Flow Chemistry

Pedro Fernandes et al.

Summary: In recent years, there has been a growing interest in using biocatalysts in flow reactors, combining the high selectivity and mild operation conditions of biocatalysis with the advantages of flow chemistry. This approach allows for efficient cascade reactions, mimics natural metabolic pathways, and provides opportunities for novel production pathways.

PROCESSES (2021)

Add to Collection
Article Biochemistry & Molecular Biology

Evaluation of Different Ionic Liquids as Additives in the Immobilization of Lipase CAL B by Sol-Gel Technique

Aline Matuella Moreira Ficanha et al.

Summary: The study focuses on immobilizing Candida antarctica B lipase using the sol-gel technique with ionic liquids as additives. It was found that ionic liquids were effective additives in the enzymatic immobilization process, with xerogel showing greater thermal stability and more use cycles compared to aerogel.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2021)

Add to Collection
Article Chemistry, Multidisciplinary

Chemically Triggered Changes in Mechanical Properties of Responsive Liquid Crystal Polymer Networks with Immobilized Urease

Albert Velasco Abadia et al.

Summary: Researchers successfully introduced enzymes as molecular triggers into LCN materials, enabling them to respond to chemical stimuli in a specific way, further expanding the potential applications of LCNs. The study found that the introduction of enzymes can trigger shape transformation in LCNs, and modulate the mechanical properties of the material through the formation of hydrogen bonds.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

Add to Collection
Review Agricultural Engineering

Magnetic micro-macro biocatalysts applied to industrial bioprocesses

Jon Del Arco et al.

Summary: The use of magnetic biocatalysts simplifies operational processing, increases efficiency, and reduces costs. Small-size matrices as carriers for enzyme immobilization help maximize surface area and catalysts loading, while reducing diffusion limitations.

BIORESOURCE TECHNOLOGY (2021)

Add to Collection
Article Biotechnology & Applied Microbiology

Effect of amine length in the interference of the multipoint covalent immobilization of enzymes on glyoxyl agarose beads

Roberto Morellon-Sterling et al.

Summary: Enzymes such as trypsin, chymotrypsin, penicillin G acylase, and ficin extract were stabilized through immobilization on glyoxyl agarose with the addition of different aliphatic compounds containing a primary amine group. The presence of amines was found to decrease the enzyme-support attachment covalently and reduce enzyme stability, with octyl amine having the most significant effect. The steric hindrance caused by aminated compounds interacting with aldehyde groups on the enzyme/support multi-reaction plays a key role in decreasing enzyme stability, even with just 1 mM of aminated compounds.

JOURNAL OF BIOTECHNOLOGY (2021)

Add to Collection
Review Biochemistry & Molecular Biology

Production of Bulk Chemicals with Biocatalysis: Drivers and Challenges Reflected in Recent Industrial Granted Patents (2015-2020)

Nadia Guajardo et al.

Summary: The application of biocatalysis and White Biotechnology in the production of bulk compounds and low-added value products in chemical sectors has been gaining importance. The use of biocatalysis in these areas offers energy savings, cleaner products, reduced generation of by-products, and manageable wastes. Recent industrial patents related to biocatalysis and bulk chemicals have been explored, showing innovation in enzyme identification, improved immobilization methods, and novel reactor designs for enhanced performance and economics.

MOLECULES (2021)

Add to Collection
Article Chemistry, Physical

Computationally Driven Rational Design of Substrate Promiscuity on Serine Ester Hydrolases

Sergi Roda et al.

Summary: Through studying enzymes with narrow substrate spectra, it was demonstrated that active site parameters can be manipulated to alter substrate specificity and design new enzyme variants with broader substrate promiscuity.

ACS CATALYSIS (2021)

Add to Collection
Review Acoustics

Application of multi-frequency power ultrasound in selected food processing using large-scale reactors: A review

Baoguo Xu et al.

Summary: This paper discusses the application of ultrasound in food processing, specifically in enzymolysis of protein and washing of fruits and vegetables. Ultrasound can improve protein enzymolysis and enhance the cleanliness of fruits and vegetables surfaces. Future developments in ultrasound technology for these applications are also considered.

ULTRASONICS SONOCHEMISTRY (2021)

Add to Collection
Article Chemistry, Physical

Intraparticle Kinetics Unveil Crowding and Enzyme Distribution Effects on the Performance of Cofactor-Dependent Heterogeneous Biocatalysts

Eleftheria Diamanti et al.

Summary: This study conducted multidimensional kinetic analysis on self-sufficient heterogeneous biocatalysts, revealing a thermodynamic equilibrium and the impact of enzyme spatial organization on enzyme performance. Through fluorescence recovery after photobleaching measurements and time-lapse fluorescence microscopy, the study provides insights into the in operando functionality of enzymes within confined spaces.

ACS CATALYSIS (2021)

Add to Collection
Article Chemistry, Physical

Robust nanocarriers to engineer nanobiocatalysts for bioprocessing applications

Muhammad Bilal et al.

Summary: The synergistic integration of bio-catalysis engineering with nanostructured materials has led to the development of nanobiocatalysis (NBC), offering significant considerations for designing robust catalysts at the nanoscale. NBC provides improved catalytic characteristics for various bioprocessing applications, with unique structural and functional entities of nanostructured materials contributing significantly in mimicking enzyme microenvironments. Additionally, NBC is rapidly growing and is expected to become a powerful norm in bio-catalysis, offering controlled features such as selectivity, stability, and cost-effectiveness.

ADVANCES IN COLLOID AND INTERFACE SCIENCE (2021)

Add to Collection
Review Chemistry, Multidisciplinary

Recent Advances in Biocatalysis with Chemical Modification and Expanded Amino Acid Alphabet

Amol D. Pagar et al.

Summary: The two main strategies for enzyme engineering, directed evolution and rational design, have been widely used to improve the intrinsic activities of proteins. However, the limited chemical diversity of biopolymers, restricted to 20 canonical amino acids, hinders the creation of novel enzymes. To overcome this, researchers have been devoted to expanding the protein sequence space through chemical modifications and/or incorporation of noncanonical amino acids.

CHEMICAL REVIEWS (2021)

Add to Collection
Article Chemistry, Physical

Catalyst Replacement Policy on Multienzymatic Systems: Theoretical Study in the One-Pot Sequential Batch Production of Lactofructose Syrup

Pablo Silva et al.

Summary: The study examines the behavior of one-pot sequential batch production of lactofructose syrup with individually immobilized enzymes, aiming to evaluate and design a strategy of replacement based on their stabilities. The results demonstrate that a proper biocatalyst replacement strategy can increase the productivity of the process.

CATALYSTS (2021)

Add to Collection
Article Chemistry, Multidisciplinary

Engineered Ribosyl-1-Kinase Enables Concise Synthesis of Molnupiravir, an Antiviral for COVID-19

John A. McIntosh et al.

Summary: To meet the high demand and urgency for the investigational antiviral agent molnupiravir in treating COVID-19, a novel biocatalytic cascade was developed with engineered enzymes to significantly shorten the synthesis time by 70% and increase yield by approximately 7-fold compared to the initial route. This biocatalytic approach is expected to have broad applications for simplifying nucleoside synthesis.

ACS CENTRAL SCIENCE (2021)

Add to Collection
Article Biochemistry & Molecular Biology

The β-galactosidase immobilization protocol determines its performance as catalysts in the kinetically controlled synthesis of lactulose

Carlos Alberto Chaves Girao Neto et al.

Summary: This study demonstrates that the immobilization method significantly affects the performance and stability of beta-galactosidase in the synthesis of lactulose. Temperature and biocatalyst choice play a crucial role in the synthesis efficiency, with agarose and agarose coated with polyethylenimine showing better performance under suitable conditions. However, chitosan activated with glutaraldehyde performed relatively poorly in comparison.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

Add to Collection
Article Materials Science, Ceramics

Strategy to enhance catalytic activity and stability of sol-gel oxidoreductases

Nur Atikah Mohidem et al.

Summary: By entrapping oxidoreductases in sol-gel silica, their catalytic activity, stability, and reusability were significantly enhanced, showing promising potential for industrial applications.

JOURNAL OF SOL-GEL SCIENCE AND TECHNOLOGY (2021)

Add to Collection
Article Chemistry, Multidisciplinary

Faster Surface Ligation Reactions Improve Immobilized Enzyme Structure and Activity

Andres F. Chaparro Sosa et al.

Summary: The efficiency of ligation reactions plays a crucial role in maintaining the activity and structure of enzymes on solid surfaces. Limiting enzyme exploration of surfaces may overcome the challenge of enzyme inactivation during integration. Increasing the rate constant of surface ligation reactions can enhance the probability of immobilization with reactive surface sites.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2021)

Add to Collection
Article Chemistry, Multidisciplinary

Intensification of oxygen-dependent biotransformations catalyzed by immobilized enzymes

Alvaro Lorente-Arevalo et al.

Summary: Oxidative biotransformations play a vital role in the fine chemical industry and renewable feedstock valorization, but face challenges in reaction kinetics, oxygen transfer, and process stability. An interdisciplinary approach focused on heterogeneous biocatalyst engineering could be strategically valuable for advancing this field.

CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY (2021)

Add to Collection
Article Chemistry, Multidisciplinary

Biocatalytic process intensification via efficient biocatalyst immobilization, miniaturization, and process integration

Polona Znidarsic-Plazl

Summary: The potential of biocatalytic processes in industrial production is hindered by challenges such as the need for highly active catalysts, efficient cofactor regeneration, and prevention of deactivation; Enzyme and cell immobilization are crucial for process feasibility; Intensification of biocatalytic processes can be achieved through reactor miniaturization, continuous operation, and integration of cascade reactions.

CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY (2021)

Add to Collection
Article Chemistry, Multidisciplinary

Green Process for the Enzymatic Synthesis of Aroma Compounds Mediated by Lipases Entrapped in Tailored Sol-Gel Matrices

Adrian Ioan Dudu et al.

Summary: This study presents a simple enzymatic approach for the synthesis of short-chained flavor esters with high efficiency. By optimizing reaction conditions, the alcohol/enzyme ratio was reduced from 100:1 to 25:1, leading to significant improvements in reaction yields.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2021)

Add to Collection
Article Chemistry, Physical

Immobilization of the Peroxygenase from Agrocybe aegerita. The Effect of the Immobilization pH on the Features of an Ionically Exchanged Dimeric Peroxygenase

Diego Carballares et al.

Summary: This study investigated the immobilization of rAaeUPO enzyme from Agrocybe aegerita. The findings showed that the immobilization pH significantly influences the stability and activity of the immobilized enzyme.

CATALYSTS (2021)

Add to Collection
Article Chemistry, Multidisciplinary

Biocatalysis, sustainability, and industrial applications: Show me the metrics

Pablo Dominguez de Maria

Summary: Biocatalysis is considered an environmentally friendly industrial toolbox for organic synthesis, with recent developments focusing on generating greener applications. High substrate loadings in aqueous-based processes, solvent-free reactions, and the use of biogenic solvents are key methods discussed. Solvent-free systems are seen as superior in terms of greenness and industrial use, especially when combined with flow systems and recycling of solvents and water. Providing metrics is crucial to assess biotransformations and drive further research for improved systems.

CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY (2021)

Add to Collection
Article Nanoscience & Nanotechnology

Understanding Design Rules for Optimizing the Interface between Immobilized Enzymes and Random Copolymer Brushes

Hector Sanchez-Moran et al.

Summary: "The long-term goal of biotechnology is to develop design rules for stabilizing enzymes when immobilized to materials. By investigating the stabilization of lipases immobilized on PEGMA and SBMA copolymer brush surfaces, it was found that the stability of the lipases at elevated temperatures depended on the fraction of PEGMA in the brush layer. This understanding can lead to the rational tuning of enzyme-material interfaces for reliable ruggedization of enzymes under extreme conditions."

ACS APPLIED MATERIALS & INTERFACES (2021)

Add to Collection
Article Chemistry, Applied

Modification of the Enantioselectivity of Biocatalytic meso-Desymmetrization for Synthesis of Both Enantiomers of cis-1,2-Disubstituted Cyclohexane by Amidase Engineering

Hui-Juan Hu et al.

Summary: Both enantiomers of cis-1,2-disubstituted cyclohexane were obtained enantioselectively through engineered amidase-catalyzed desymmetrization of meso carbocyclic 1,2-dicarboxamides under mild conditions. The amplification and reversal of enantioselectivity of amidase was quickly achieved through generating and testing only 8 variants based on the suggested enzyme-substrate binding model by molecular docking. The synthetic application of biocatalytic meso-desymmetrization was demonstrated by straightforward transformation of the products to enantiomerically pure heterocyclic compounds.

ADVANCED SYNTHESIS & CATALYSIS (2021)

Add to Collection
Article Biochemistry & Molecular Biology

Effect of Tris Buffer in the Intensity of the Multipoint Covalent Immobilization of Enzymes in Glyoxyl-Agarose Beads

Sabrina Ait Braham et al.

Summary: The presence of Tris during immobilization was found to reduce the stability of enzymes, particularly for immobilized PGA, possibly due to the homogeneous distribution of Lys groups in PGA compared to trypsin and chymotrypsin.

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY (2021)

Add to Collection
Article Biochemical Research Methods

Solid-Phase Assembly of Multienzyme Systems into Artificial Cellulosomes

Nicoll Zeballos et al.

Summary: This study presents a bioinspired solid-phase assembly method for a multienzyme system, where alcohol dehydrogenase and omega-transaminase were coimmobilized on agarose porous microbeads to form a scaffolded enzymatic cellulosome. By physically colocalizing the enzymes within the microbeads, the conversion yield of amines was significantly improved compared to segregated immobilized system and free enzymes, demonstrating a new concept for organizing multienzyme systems at the nanoscale within solid and porous immobilization carriers.

BIOCONJUGATE CHEMISTRY (2021)

Add to Collection
Review Biochemical Research Methods

Large-scale production of enzymes for biotechnology uses

Aneesa Fasim et al.

Summary: Enzymes are biocatalysts that accelerate chemical reactions to produce valuable products. Biotechnology has expanded the use of traditional enzymes into industries such as food, beverage, personal care, and agriculture. In recent years, there has been exponential growth of enzyme applications in the biotech industry, especially hydrolytic microbial enzymes used in industrial bioprocesses.

CURRENT OPINION IN BIOTECHNOLOGY (2021)

Add to Collection
Review Chemistry, Organic

Cascade Biocatalytic Processes through Combined Cross-linked Enzyme Aggregates

Nicolctta Gaggero et al.

Summary: Cascade reactions catalyzed by immobilized multi-enzymatic systems, particularly using combi-CLEAs as a carrier-free immobilization strategy, provide a simple and cost-effective approach to enhance enzyme stability, facilitate biocatalyst recovery and recycling, and enable practical industrial applications at a multigram scale.

CURRENT ORGANIC CHEMISTRY (2021)

Add to Collection
Article Thermodynamics

Extraction of antibiotics using aqueous two-phase systems based on ethyl lactate and thiosulphate salts

Malgorzata E. Zakrzewska et al.

Summary: This study utilized ethyl lactate to form ATPS for the separation of antibiotics, achieving extraction with thiosulfate salts. Ethyl lactate-based ATPS serves as a novel green platform for antibiotic extraction in surface waters and fermentation broths.

FLUID PHASE EQUILIBRIA (2021)

Add to Collection
Review Engineering, Chemical

Ultrasound-Assisted Intensification of Activity of Free and Immobilized Enzymes: A Review

Priya et al.

Summary: Ultrasound has shown potential in enhancing enzymatic activity by altering enzyme structure under mild controlled conditions. Optimization of ultrasound and process parameters can lead to increased enzymatic activity and improved catalytic efficiency, benefiting enzyme-based applications.

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH (2021)

Add to Collection
Review Biochemistry & Molecular Biology

Cellulose-deconstruction potential of nano-biocatalytic systems: A strategic drive from designing to sustainable applications of immobilized cellulases

Sarmad Ahmad Qamar et al.

Summary: The utilization of nanostructured materials along with polymers as support carriers for enzyme immobilization has gained significant attention, leading to the development of robust matrices that enhance the efficacy of biocatalysts. Nano-supported catalysts possess unique structural, physicochemical, and functional attributes that hold promise in overcoming limitations associated with traditional biocatalysts.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

Add to Collection
Review Biochemistry & Molecular Biology

Bioactive peptides from fisheries residues: A review of use of papain in proteolysis reactions

Veymar G. Tacias-Pascacio et al.

Summary: The article focuses on the application of papain in the hydrolysis of proteins from fish residues, converting the residual material into interesting products with antioxidant, nutritional, and functional activities.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2021)

Add to Collection
Article Chemistry, Physical

Reversal and Amplification of the Enantioselectivity of Biocatalytic Desymmetrization toward Meso Heterocyclic Dicarboxamides Enabled by Rational Engineering of Amidase

Yu-Fei Ao et al.

Summary: This study demonstrates efficient biocatalytic desymmetrization of meso O-heterocyclic dicarboxamides for synthesizing both antipodes of functionalized cyclic motifs, with yields up to 99.5% ee. The engineered biocatalyst shows wide substrate promiscuity, high efficiency, and excellent enantioselectivity.

ACS CATALYSIS (2021)

Add to Collection
Review Chemistry, Physical

An Overview of Cytochrome P450 Immobilization Strategies for Drug Metabolism Studies, Biosensing, and Biocatalytic Applications: Challenges and Opportunities

Donya Valikhani et al.

Summary: Cytochrome P450s are a large superfamily of heme proteins with important applications in drug metabolism studies and biocatalytic transformations. Immobilization of P450 enzymes on biosensing platforms and in biocatalytic reactions plays a key role in improving operational stability. Various methods and materials have been developed to address the challenges associated with the unique characteristics of P450s in different applications.

ACS CATALYSIS (2021)

Add to Collection
Article Chemistry, Multidisciplinary

Streamlining Design, Engineering, and Applications of Enzymes for Sustainable Biocatalysis

Roger A. Sheldon et al.

Summary: This Perspective highlights the expansion of biocatalysis in industrial organic synthesis through the optimization of biocatalyst and bioprocess engineering, enabling cost-effective and sustainable production of APIs with high selectivity.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2021)

Add to Collection
Article Chemistry, Multidisciplinary

Advantages of Supports Activated with Divinyl Sulfone in Enzyme Coimmobilization: Possibility of Multipoint Covalent Immobilization of the Most Stable Enzyme and Immobilization via Ion Exchange of the Least Stable Enzyme

Roberto Morellon-Sterling et al.

Summary: A new strategy was developed using VS-agarose beads to coimmobilize enzymes with different stabilities, allowing for the reuse of the most stable immobilized enzyme. Two model combi-biocatalysts were prepared and tested, demonstrating the stability and potential for repeated use of the immobilized enzymes. The results showed that the immobilized proteases were more stable than the immobilized lactase, with potential for practical applications in biocatalysis.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2021)

Add to Collection
Article Chemistry, Physical

Encapsulation of Combi-CLEAs of Glycosidases in Alginate Beads and Polyvinyl Alcohol for Wine Aroma Enhancement

Luigi Tavernini et al.

Summary: The study demonstrates that the aromatic expression of wines can be enhanced by using combi-CLEAs of glycosidases, which are immobilized and encapsulated in polyvinyl alcohol for stability and recovery. The polyvinyl-encapsulated combi-CLEAs exhibit superior stability and activity in white wine, allowing for easy recovery and reuse.

CATALYSTS (2021)

Add to Collection
Review Materials Science, Biomaterials

Immobilized enzymes in inorganic hybrid nanoflowers for biocatalytic and biosensing applications

Xiao Liang et al.

Summary: Enzyme immobilization in inorganic hybrid nanoflowers is a biomimetic mineralization method with mild reaction conditions, which helps to obtain ideal biocatalysts. Immobilized enzymes in inorganic hybrid nanoflowers have been successfully used in biocatalysis, producing medical intermediates and biodiesel, as well as in developing biosensors for detecting toxic substances and disease biomarkers.

JOURNAL OF MATERIALS CHEMISTRY B (2021)

Add to Collection
Article Chemistry, Multidisciplinary

Framework of the kinetic analysis of O2-dependent oxidative biocatalysts for reaction intensification

Alvaro Lorente-Arevalo et al.

Summary: The study introduces an analysis framework combining differential and integral methods for studying the kinetics of oxygen-dependent enzymes. By utilizing initially dissolved oxygen and working only with liquid suspensions, the methodology is applicable to important case studies in modern green biooxidations.

REACTION CHEMISTRY & ENGINEERING (2021)

Add to Collection
Review Chemistry, Physical

Solvent-free esterifications mediated by immobilized lipases: a review from thermodynamic and kinetic perspectives

Ronaldo Rodrigues Sousa et al.

Summary: Esters are important compounds in industry, and using biocatalysis for their synthesis in solvent-free systems offers economic and environmental advantages. However, optimizing reactions in these systems is challenging due to the complexity and variety of phenomena involved.

CATALYSIS SCIENCE & TECHNOLOGY (2021)

Add to Collection
Review Chemistry, Multidisciplinary

Recent trends in biocatalysis

Dong Yi et al.

Summary: Biocatalysis has made revolutionary progress in the past century, benefiting from the integration of multidisciplinary technologies and the development of robust biocatalysts through protein engineering. The network of natural enzymatic synthesis pathways and artificially designed enzymatic cascades have been gradually constructed. Future development will move towards the integration of new technologies, intelligent manufacturing, and enzymatic total synthesis.

CHEMICAL SOCIETY REVIEWS (2021)

Add to Collection
Review Chemistry, Physical

Process intensification using immobilized enzymes for the development of white biotechnology

Harshada M. Salvi et al.

Summary: The increasing demand for sustainable processes is driving the growth of biotechnological industries, with white biotechnology playing a key role in enhancing enzyme production and stability through process intensification techniques. Biocatalysis, particularly when combined with immobilization of enzymes, offers advantages over chemical catalysis, leading to more sustainable and economical reactions. Integration of biocatalysis with process intensification techniques can further boost productivity and yield, ultimately strengthening the bio-based economy.

CATALYSIS SCIENCE & TECHNOLOGY (2021)

Add to Collection
Article Chemistry, Physical

Self-sufficient asymmetric reduction of β-ketoesters catalysed by a novel and robust thermophilic alcohol dehydrogenase co-immobilised with NADH

Alejandro H. Orrego et al.

Summary: The research demonstrates the importance of asymmetric reduction of beta-ketoesters with cell-free enzymes in the synthesis of functional products. By using a novel thermophilic enzyme, it was possible to achieve successful reuse of both the enzyme and co-immobilised cofactor in up to 10 reaction cycles.

CATALYSIS SCIENCE & TECHNOLOGY (2021)

Add to Collection
Review Chemistry, Multidisciplinary

Flow biocatalysis 101: design, development and applications

Ana I. Benitez-Mateos et al.

Summary: This tutorial review introduces the key concepts of flow biocatalysis, guides the reader through its advantages, and highlights current trends in the field to encourage innovative applications of enzymes in flow reactors.

REACTION CHEMISTRY & ENGINEERING (2021)

Add to Collection
Article Chemistry, Physical

Development of recyclable magnetic cross-linked enzyme aggregates for the synthesis of high value rare sugar d-tagatose in aqueous phase catalysis

Shushil Kumar Rai et al.

Summary: This study successfully synthesized a high value rare sugar d-tagatose using recyclable magnetic catalysts and developed two novel magnetic cross-linked enzyme aggregates for efficient transformation of d-galactose and lactose. The immobilized catalysts showed high catalytic activity and reusability potential, indicating promising catalytic potential for the synthesis of d-tagatose.

CATALYSIS SCIENCE & TECHNOLOGY (2021)

Add to Collection
Review Chemistry, Multidisciplinary

New frontiers in enzyme immobilisation: robust biocatalysts for a circular bio-based economy†

Roger A. Sheldon et al.

Summary: This tutorial review focuses on recent advances in technologies for enzyme immobilisation, enabling their cost-effective use in the bio-based economy and continuous processing in general. The application of enzymes, particularly in aqueous media, is generally on a single use, throw-away basis which is neither cost-effective nor compatible with a circular economy concept. This shortcoming can be overcome by immobilising the enzyme as an insoluble recyclable solid, that is as a heterogeneous catalyst.

CHEMICAL SOCIETY REVIEWS (2021)

Add to Collection
Review Chemistry, Multidisciplinary

Applied biocatalysis beyond just buffers - from aqueous to unconventional media. Options and guidelines

Morten M. C. H. van Schie et al.

Summary: The use of non-aqueous solvents in biocatalysis is a potential solution to increase product concentrations and decrease waste formation. Additionally, engineering protein structures can improve the efficiency of biocatalysts, while careful selection of solvent types and systems is crucial.

GREEN CHEMISTRY (2021)

Add to Collection
Review Nanoscience & Nanotechnology

Nature Inspired Multienzyme Immobilization: Strategies and Concepts

Nidhi C. Dubey et al.

Summary: This article discusses the importance of enzyme immobilization design and metabolic channeling in biological systems, introduces methods for multienzyme immobilization, discusses various concepts for enhancing enzyme catalytic efficiency, and summarizes the progress in nature-inspired multienzyme assembly over the past decade.

ACS APPLIED BIO MATERIALS (2021)

Add to Collection
Review Materials Science, Biomaterials

The study and application of biomolecules in deep eutectic solvents

Jennifer A. Kist et al.

Summary: Deep eutectic solvents are gaining attention as potential replacements for traditional organic solvents due to their biodegradability, low toxicity, and cost-effective preparation from widely available natural feedstocks.

JOURNAL OF MATERIALS CHEMISTRY B (2021)

Add to Collection
Review Biochemistry & Molecular Biology

Parameters necessary to define an immobilized enzyme preparation

Joseph Boudrant et al.

PROCESS BIOCHEMISTRY (2020)

Add to Collection
Review Biochemistry & Molecular Biology

On the relationship between structure and catalytic effectiveness in solid surface-immobilized enzymes: Advances in methodology and the quest for a single-molecule perspective

Juan M. Bolivar et al.

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS (2020)

Add to Collection
Article Biotechnology & Applied Microbiology

Glycosynthase reaction meets the flow: Continuous synthesis of lacto-N-triose II by engineered β-hexosaminidase immobilized on solid support

Lucija Ruzic et al.

BIOTECHNOLOGY AND BIOENGINEERING (2020)

Add to Collection
Review Chemistry, Physical

Thermodynamically and Kinetically Controlled Reactions in Biocatalysis - from Concepts to Perspectives

Stefan R. Marsden et al.

CHEMCATCHEM (2020)

Add to Collection
Review Chemistry, Inorganic & Nuclear

Metal-organic frameworks as novel matrices for efficient enzyme immobilization: An update review

Shan Liang et al.

COORDINATION CHEMISTRY REVIEWS (2020)

Add to Collection
Article Biotechnology & Applied Microbiology

Pectin lyase immobilization using the glutaraldehyde chemistry increases the enzyme operation range

Lucas Dal Magro et al.

ENZYME AND MICROBIAL TECHNOLOGY (2020)

Add to Collection
Article Biotechnology & Applied Microbiology

Modulating the properties of the lipase from Thermomyces lanuginosus immobilized on octyl agarose beads by altering the immobilization conditions

Yuliya Lokha et al.

ENZYME AND MICROBIAL TECHNOLOGY (2020)

Add to Collection
Article Biochemistry & Molecular Biology

Glycosylation of hyperthermostable designer cellulosome components yields enhanced stability and cellulose hydrolysis

Amaranta Kahn et al.

FEBS JOURNAL (2020)

Add to Collection
Article Thermodynamics

Aqueous two-phase systems: Towards novel and more disruptive applications

Jorge F. B. Pereira et al.

FLUID PHASE EQUILIBRIA (2020)

Add to Collection
Article Biotechnology & Applied Microbiology

Immobilized lipase-CLEA aggregates encapsulated in lentikats® as robust biocatalysts for continuous processes in deep eutectic solvents

Nadia Guajardo et al.

JOURNAL OF BIOTECHNOLOGY (2020)

Add to Collection
Article Engineering, Environmental

Supported growth of inorganic-organic nanoflowers on 3D hierarchically porous nanofibrous membrane for enhanced enzymatic water treatment

Mengying Luo et al.

JOURNAL OF HAZARDOUS MATERIALS (2020)

Add to Collection
Article Chemistry, Multidisciplinary

Reduced Enzyme Dynamics upon Multipoint Covalent Immobilization Leads to Stability-Activity Trade-off

James S. Weltz et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2020)

Add to Collection
Review Biochemistry & Molecular Biology

Recent advances in nano-carrier immobilized enzymes and their applications

Dong-Mei Liu et al.

PROCESS BIOCHEMISTRY (2020)

Add to Collection
Article Engineering, Environmental

Magnetic nanoparticles encapsulated laccase nanoflowers: evaluation of enzymatic activity and reusability for degradation of malachite green

Tingting Sun et al.

WATER SCIENCE AND TECHNOLOGY (2020)

Add to Collection
Article Biotechnology & Applied Microbiology

Effects of Enzyme Loading and Immobilization Conditions on the Catalytic Features of Lipase From Pseudomonas fluorescens Immobilized on Octyl-Agarose Beads

Sara Arana-Pena et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2020)

Add to Collection
Article Chemistry, Physical

Expanding the enzyme universe with genetically encoded unnatural amino acids

Ivana Drienovska et al.

NATURE CATALYSIS (2020)

Add to Collection
Article Chemistry, Physical

Genetically engineered proteins with two active sites for enhanced biocatalysis and synergistic chemo- and biocatalysis

Sandra Alonso et al.

NATURE CATALYSIS (2020)

Add to Collection
Article Chemistry, Physical

Synergistic catalysis in an artificial enzyme by simultaneous action of two abiological catalytic sites

Zhi Zhou et al.

NATURE CATALYSIS (2020)

Add to Collection
Article Chemistry, Physical

Co-immobilization and Colocalization of Multi-Enzyme Systems for the Cell-Free Biosynthesis of Aminoalcohols

Susana Velasco-Lozano et al.

CHEMCATCHEM (2020)

Add to Collection
Review Biochemistry & Molecular Biology

Protein immobilization technology for flow biocatalysis

Maria Romero-Fernandez et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2020)

Add to Collection
Article Biochemistry & Molecular Biology

Entrapment of porous cross-linked enzyme aggregates of maltogenic amylase from Bacillus lehensis G1 into calcium alginate for maltooligosaccharides synthesis

Noor Namirah Nawawi et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

Add to Collection
Review Biochemistry & Molecular Biology

Enzyme embedded metal organic framework (enzyme-MOF): De novo approaches for immobilization

Shamraja S. Nadar et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

Add to Collection
Article Biotechnology & Applied Microbiology

Use of polyethylenimine to produce immobilized lipase multilayers biocatalysts with very high volumetric activity using octyl-agarose beads: Avoiding enzyme release during multilayer production

Sara Arana-Pena et al.

ENZYME AND MICROBIAL TECHNOLOGY (2020)

Add to Collection
Article Biochemistry & Molecular Biology

Coimmobilization and colocalization of a glycosyltransferase and a sucrose synthase greatly improves the recycling of UDP-glucose: Glycosylation of resveratrol 3-O-β-D-glucoside

Lara Trobo-Maseda et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

Add to Collection
Article Chemistry, Multidisciplinary

Engineering the Composition of Heterogeneous Lipid Bilayers to Stabilize Tethered Enzymes

Andres F. Chaparro Sosa et al.

ADVANCED MATERIALS INTERFACES (2020)

Add to Collection
Review Chemistry, Physical

Enzyme-Coated Micro-Crystals: An Almost Forgotten but Very Simple and Elegant Immobilization Strategy

Rodolpho R. C. Monteiro et al.

CATALYSTS (2020)

Add to Collection
Article Biotechnology & Applied Microbiology

A mild intensity of the enzyme -support multi -point attachment promotes the optimal stabilization of mesophilic multimeric enzymes: Amine oxidase from Pisum sativum

Paz Garcia-Garcia et al.

JOURNAL OF BIOTECHNOLOGY (2020)

Add to Collection
Review Biotechnology & Applied Microbiology

Immobilization of Multi-Enzymes on Support Materials for Efficient Biocatalysis

Kongliang Xu et al.

FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY (2020)

Add to Collection
Article Engineering, Environmental

Enzyme synthesis of cephalexin in continuous-flow microfluidic device in ATPS environment

Lucie Vobecka et al.

CHEMICAL ENGINEERING JOURNAL (2020)

Add to Collection
Review Biochemistry & Molecular Biology

Use of Alcalase in the production of bioactive peptides: A review

Veymar G. Tacias-Pascacio et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

Add to Collection
Article Chemistry, Physical

Multi-Combilipases: Co-Immobilizing Lipases with Very Different Stabilities Combining Immobilization via Interfacial Activation and Ion Exchange. The Reuse of the Most Stable Co-Immobilized Enzymes after Inactivation of the Least Stable Ones

Sara Arana-Pena et al.

CATALYSTS (2020)

Add to Collection
Review Chemistry, Multidisciplinary

Characterization and evaluation of immobilized enzymes for applications in flow reactors

Juan M. Bolivar et al.

CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY (2020)

Add to Collection
Article Nanoscience & Nanotechnology

Design of the Enzyme-Carrier Interface to Overcome the O2 and NADH Mass Transfer Limitations of an Immobilized Flavin Oxidase

Ana Benitez-Mateos et al.

ACS APPLIED MATERIALS & INTERFACES (2020)

Add to Collection
Article Nanoscience & Nanotechnology

Vortex Fluidic-Mediated Fabrication of Fast Gelated Silica Hydrogels with Embedded Laccase Nanoflowers for Real-Time Biosensing under Flow

Xuan Luo et al.

ACS APPLIED MATERIALS & INTERFACES (2020)

Add to Collection
Article Biochemistry & Molecular Biology

Improved immobilization of lipase from Thermomyces lanuginosus on a new chitosan-based heterofunctional support: Mixed ion exchange plus hydrophobic interactions

Nicole S. Okura et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2020)

Add to Collection
Review Chemistry, Multidisciplinary

The rise of continuous flow biocatalysis - fundamentals, very recent developments and future perspectives

Piera De Santis et al.

REACTION CHEMISTRY & ENGINEERING (2020)

Add to Collection
Review Biotechnology & Applied Microbiology

Developing Aspergillus niger as a cell factory for food enzyme production

Cen Li et al.

BIOTECHNOLOGY ADVANCES (2020)

Add to Collection
Review Chemistry, Physical

Enzyme production ofd-gluconic acid and glucose oxidase: successful tales of cascade reactions

Jakub F. Kornecki et al.

CATALYSIS SCIENCE & TECHNOLOGY (2020)

Add to Collection
Article Biochemical Research Methods

Towards the sustainable production of bulk-chemicals using biotechnology

John M. Woodley

NEW BIOTECHNOLOGY (2020)

Add to Collection
Review Chemistry, Multidisciplinary

Deep eutectic solvents and their applications as green solvents

Filippo Maria Perna et al.

CURRENT OPINION IN GREEN AND SUSTAINABLE CHEMISTRY (2020)

Add to Collection
Review Microbiology

Aspergillus nidulans: A Potential Resource of the Production of the Native and Heterologous Enzymes for Industrial Applications

Amit Kumar

INTERNATIONAL JOURNAL OF MICROBIOLOGY (2020)

Add to Collection
Article Biochemical Research Methods

Demystifying the Flow: Biocatalytic Reaction Intensification in Microstructured Enzyme Reactors

Juan M. Bolivar et al.

BIOTECHNOLOGY JOURNAL (2019)

Add to Collection
Review Biotechnology & Applied Microbiology

Developments and opportunities in fungal strain engineering for the production of novel enzymes and enzyme cocktails for plant biomass degradation

Roland S. Kun et al.

BIOTECHNOLOGY ADVANCES (2019)

Add to Collection
Review Chemistry, Physical

Reactor Selection for Effective Continuous Biocatalytic Production of Pharmaceuticals

Rowan M. Lindeque et al.

CATALYSTS (2019)

Add to Collection
Article Chemistry, Physical

CLEAs, Combi-CLEAs and Smart' Magnetic CLEAs: Biocatalysis in a Bio-Based Economy

Roger A. Sheldon

CATALYSTS (2019)

Add to Collection
Review Biotechnology & Applied Microbiology

Accelerating the implementation of biocatalysis in industry

John M. Woodley

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2019)

Add to Collection
Review Biotechnology & Applied Microbiology

Immobilization of lipases on hydrophobic supports: immobilization mechanism, advantages, problems, and solutions

Rafael C. Rodrigues et al.

BIOTECHNOLOGY ADVANCES (2019)

Add to Collection
Article Biochemistry & Molecular Biology

Immobilization and stabilization of different β-glucosidases using the glutaraldehyde chemistry: Optimal protocol depends on the enzyme

Diandra de Andrades et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

Add to Collection
Review Biotechnology & Applied Microbiology

Deep Eutectic Solvents as Efficient Solvents in Biocatalysis

Magdalena Paetzold et al.

TRENDS IN BIOTECHNOLOGY (2019)

Add to Collection
Review Chemistry, Physical

Multienzymatic Cascade Reactions via Enzyme Complex by Immobilization

Ee Taek Hwang et al.

ACS CATALYSIS (2019)

Add to Collection
Article Chemistry, Multidisciplinary

Development of Effective Lipase-Hybrid Nanoflowers Enriched with Carbon and Magnetic Nanomaterials for Biocatalytic Transformations

Renia Fotiadou et al.

NANOMATERIALS (2019)

Add to Collection
Review Chemistry, Physical

Multiscale modeling and simulations of protein adsorption: progresses and perspectives

Xuebo Quan et al.

CURRENT OPINION IN COLLOID & INTERFACE SCIENCE (2019)

Add to Collection
Article Biochemistry & Molecular Biology

Development of magnetic cross-linked peroxidase aggregates on starch as enhancement template and their application for decolorization

Atheer Awad Mehde

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

Add to Collection
Article Biochemistry & Molecular Biology

New applications of glyoxyl-octyl agarose in lipases co-immobilization: Strategies to reuse the most stable lipase

Sara Arana-Pena et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

Add to Collection
Article Biochemistry & Molecular Biology

Amination of ficin extract to improve its immobilization on glyoxyl-agarose: Improved stability and activity versus casein

El-Hocine Siar et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2019)

Add to Collection
Review Chemistry, Physical

Dextran Aldehyde in Biocatalysis: More Than a Mere Immobilization System

Veymar G. Tacias-Pascacio et al.

CATALYSTS (2019)

Add to Collection
Review Biochemistry & Molecular Biology

The Microenvironment in Immobilized Enzymes: Methods of Characterization and Its Role in Determining Enzyme Performance

Juan M. Bolivar et al.

MOLECULES (2019)

Add to Collection
Article Biotechnology & Applied Microbiology

Glycosylation influences activity, stability and immobilization of the feruloyl esterase 1a from Myceliophthora thermophila

Cyrielle Bonzom et al.

AMB EXPRESS (2019)

Add to Collection
Article Chemistry, Physical

Engineered enzymes that retain and regenerate their cofactors enable continuous-flow biocatalysis

Carol J. Hartley et al.

NATURE CATALYSIS (2019)

Add to Collection
Review Biochemical Research Methods

Filamentous fungi for the production of enzymes, chemicals and materials

Han A. B. Wosten

CURRENT OPINION IN BIOTECHNOLOGY (2019)

Add to Collection
Article Biochemistry & Molecular Biology

Considerations when Measuring Biocatalyst Performance

Mafalda Dias Gomes et al.

MOLECULES (2019)

Add to Collection
Article Biochemistry & Molecular Biology

Improving the Yields and Reaction Rate in the Ethanolysis of Soybean Oil by Using Mixtures of Lipase CLEAs

Margarita Diaz Ramos et al.

MOLECULES (2019)

Add to Collection
Article Chemistry, Physical

Deciphering the Effect of Microbead Size Distribution on the Kinetics of Heterogeneous Biocatalysts through Single-Particle Analysis Based on Fluorescence Microscopy

Emilio Munoz-Morales et al.

CATALYSTS (2019)

Add to Collection
Review Biotechnology & Applied Microbiology

Impact of immobilization technology in industrial and pharmaceutical applications

Mohamed E. Hassan et al.

3 BIOTECH (2019)

Add to Collection
Review Biotechnology & Applied Microbiology

The state-of-the-art strategies of protein engineering for enzyme stabilization

Qian Liu et al.

BIOTECHNOLOGY ADVANCES (2019)

Add to Collection
Article Biotechnology & Applied Microbiology

Process intensification for O2-dependent enzymatic transformations in continuous single-phase pressurized flow

Juan M. Bolivar et al.

BIOTECHNOLOGY AND BIOENGINEERING (2019)

Add to Collection
Review Chemistry, Applied

Biocatalysis Using Immobilized Enzymes in Continuous Flow for the Synthesis of Fine Chemicals

Matthew P. Thompson et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2019)

Add to Collection
Article Biochemistry & Molecular Biology

Biodegradation of fluorene by neoteric LDPE immobilized Pseudomonas pseudoalcaligenes NRSS3 in a packed bed bioreactor and analysis of external mass transfer correlation

R. K. Sonwani et al.

PROCESS BIOCHEMISTRY (2019)

Add to Collection
Article Multidisciplinary Sciences

Design of an invitro biocatalytic cascade for the manufacture of islatravir

Mark A. Huffman et al.

SCIENCE (2019)

Add to Collection
Review Chemistry, Physical

Industrial applications of immobilized enzymes-A review

Alessandra Basso et al.

MOLECULAR CATALYSIS (2019)

Add to Collection
Review Chemistry, Physical

Genipin as An Emergent Tool in the Design of Biocatalysts: Mechanism of Reaction and Applications

Veymar G. Tacias-Pascacio et al.

CATALYSTS (2019)

Add to Collection
Review Chemistry, Physical

Novozym 435: the perfect lipase immobilized biocatalyst?

Claudia Ortiz et al.

CATALYSIS SCIENCE & TECHNOLOGY (2019)

Add to Collection
Article Engineering, Environmental

The external mass transfer model for the hydrolysis of palm olein using immobilized lipase in a scaled-up recirculated packed-bed reactor

Wan Sieng Yeo et al.

JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING (2019)

Add to Collection
Article Nanoscience & Nanotechnology

Biobased, Internally pH-Sensitive Materials: Immobilized Yellow Fluorescent Protein as an Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Matrices

Tanja Consolati et al.

ACS APPLIED MATERIALS & INTERFACES (2018)

Add to Collection
Article Biochemistry & Molecular Biology

Rational Engineering of Multiple Active Sites in an Ester Hydrolase

Gerard Santiago et al.

BIOCHEMISTRY (2018)

Add to Collection
Review Biotechnology & Applied Microbiology

Integrating enzyme immobilization and protein engineering: An alternative path for the development of novel and improved industrial biocatalysts

Claudia Bernal et al.

BIOTECHNOLOGY ADVANCES (2018)

Add to Collection
Article Chemistry, Physical

Single-Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts

Ana I. Benitez-Mateos et al.

CHEMCATCHEM (2018)

Add to Collection
Article Chemistry, Physical

One-step Synthesis of -Keto Acids from Racemic Amino Acids by A Versatile Immobilized Multienzyme Cell-free System

Alejandro H. Orrego et al.

CHEMCATCHEM (2018)

Add to Collection
Review Chemistry, Multidisciplinary

Engineering enzyme microenvironments for enhanced biocatalysis

Louis Lancaster et al.

CHEMICAL SOCIETY REVIEWS (2018)

Add to Collection
Review Food Science & Technology

Biotechnological Applications of Proteases in Food Technology

Olga Luisa Tavano et al.

COMPREHENSIVE REVIEWS IN FOOD SCIENCE AND FOOD SAFETY (2018)

Add to Collection
Review Chemistry, Inorganic & Nuclear

Optimization protocols and improved strategies for metal-organic frameworks for immobilizing enzymes: Current development and future challenges

Jiandong Cui et al.

COORDINATION CHEMISTRY REVIEWS (2018)

Add to Collection
Article Biotechnology & Applied Microbiology

Stabilization of dimeric β-glucosidase from Aspergillus niger via glutaraldehyde immobilization under different conditions

Perla Guadalupe Vazquez-Ortega et al.

ENZYME AND MICROBIAL TECHNOLOGY (2018)

Add to Collection
Article Chemistry, Organic

Lipase Regioselective O-Acetylations of a myo-Inositol Derivative: Efficient Desymmetrization of 1,3-Di-O-benzyl-myo-inositol

Marcela F. P. Ribeiro et al.

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY (2018)

Add to Collection
Article Polymer Science

Multifunctional crosslinkable itaconic acid copolymers for enzyme immobilization

Felix Mueller et al.

EUROPEAN POLYMER JOURNAL (2018)

Add to Collection
Article Biochemistry & Molecular Biology

Immobilization of Aspergillus awamori β-glucosidase on commercial gelatin: An inexpensive and efficient process

Veronica S. Nishida et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2018)

Add to Collection
Article Biochemical Research Methods

Optimization of aqueous two-phase systems for the production of 6-aminopenicillanic acid in integrated microfluidic reactors-separators

Lucie Vobecka et al.

NEW BIOTECHNOLOGY (2018)

Add to Collection
Article Biochemistry & Molecular Biology

A new heterofunctional amino-vinyl sulfone support to immobilize enzymes: Application to the stabilization of beta-galactosidase from Aspergillus oryzae

Hadjer Zaak et al.

PROCESS BIOCHEMISTRY (2018)

Add to Collection
Article Biochemistry & Molecular Biology

Lipase catalyzed glycerolysis of ratfish liver oil at stirred tank basket reactor: A kinetic approach

Pablo Arranz-Martinez et al.

PROCESS BIOCHEMISTRY (2018)

Add to Collection
Article Biochemistry & Molecular Biology

Solid phase chemical modification of agarose glyoxyl-ficin: Improving activity and stability properties by amination and modification with glutaraldehyde

El-Hocine Siar et al.

PROCESS BIOCHEMISTRY (2018)

Add to Collection
Review Chemistry, Physical

Multienzyme Cascade Reactions-Status and Recent Advances

Josef M. Sperl et al.

ACS CATALYSIS (2018)

Add to Collection
Article Chemistry, Physical

Immobilization/Stabilization of Ficin Extract on Glutaraldehyde-Activated Agarose Beads. Variables That Control the Final Stability and Activity in Protein Hydrolyses

El-Hocine Siar et al.

CATALYSTS (2018)

Add to Collection
Article Chemistry, Multidisciplinary

Enhancing Coupled Enzymatic Activity by Colocalization on Nanoparticle Surfaces: Kinetic Evidence for Directed Channeling of Intermediates

James Nicholas Vranish et al.

ACS NANO (2018)

Add to Collection
Article Chemistry, Physical

Synthesis and continuous catalytic application of alkaline protease nanoflowers-PVA composite hydrogel

Haiyang Zhang et al.

CATALYSIS COMMUNICATIONS (2018)

Add to Collection
Review Chemistry, Multidisciplinary

A comprehensive overview of directing groups applied in metal-catalysed C-H functionalisation chemistry

Carlo Sambiagio et al.

CHEMICAL SOCIETY REVIEWS (2018)

Add to Collection
Review Biochemistry & Molecular Biology

Magnetic-metal organic framework (magnetic-MOF): A novel platform for enzyme immobilization and nanozyme applications

Shamraja S. Nadar et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2018)

Add to Collection
Article Chemistry, Applied

Scoping Biocatalyst Performance Using Reaction Trajectory Analysis

Mathias Nordblad et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2018)

Add to Collection
Article Biochemical Research Methods

A Synthetic Reaction Cascade Implemented by Colocalization of Two Proteins within Catalytically Active Inclusion Bodies

Vera D. Jaeger et al.

ACS SYNTHETIC BIOLOGY (2018)

Add to Collection
Article Chemistry, Multidisciplinary

Self-Sufficient Flow-Biocatalysis by Coimmobilization of Pyridoxal 5′-Phosphate and ω-Transaminases onto Porous Carriers

Ana I. Benitez-Mateos et al.

ACS SUSTAINABLE CHEMISTRY & ENGINEERING (2018)

Add to Collection
Article Chemistry, Physical

Stabilization of Enzymes by Multipoint Covalent Attachment on Aldehyde-Supports: 2-Picoline Borane as an Alternative Reducing Agent

Alejandro H. Orrego et al.

CATALYSTS (2018)

Add to Collection
Article Chemistry, Physical

Rice Husk as an Inexpensive Renewable Immobilization Carrier for Biocatalysts Employed in the Food, Cosmetic and Polymer Sectors

Marco Cespugli et al.

CATALYSTS (2018)

Add to Collection
Article Chemistry, Multidisciplinary

Directed Evolution: Bringing New Chemistry to Life

Frances H. Arnold

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2018)

Add to Collection
Review Chemistry, Multidisciplinary

Engineering more stable proteins

Romas Kazlauskas

CHEMICAL SOCIETY REVIEWS (2018)

Add to Collection
Article Biochemistry & Molecular Biology

Sustainable and Continuous Synthesis of Enantiopure l-Amino Acids by Using a Versatile Immobilised Multienzyme System

Susana Velasco-Lozano et al.

CHEMBIOCHEM (2018)

Add to Collection
Article Chemistry, Multidisciplinary

Co-immobilized Phosphorylated Cofactors and Enzymes as Self-Sufficient Heterogeneous Biocatalysts for Chemical Processes

Susana Velasco-Lozano et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2017)

Add to Collection
Article Chemistry, Physical

Constructing Biocatalytic Cascades: In Vitro and in Vivo Approaches to de Novo Multi-Enzyme Pathways

Scott P. France et al.

ACS CATALYSIS (2017)

Add to Collection
Article Biotechnology & Applied Microbiology

Improved stability of immobilized lipases via modification with polyethylenimine and glutaraldehyde

Hadjer Zaak et al.

ENZYME AND MICROBIAL TECHNOLOGY (2017)

Add to Collection
Article Biotechnology & Applied Microbiology

Relevance of substrates and products on the desorption of lipases physically adsorbed on hydrophobic supports

Jose J. Virgen-Ortiz et al.

ENZYME AND MICROBIAL TECHNOLOGY (2017)

Add to Collection
Article Biotechnology & Applied Microbiology

Effect of protein load on stability of immobilized enzymes

Laura Fernandez-Lopez et al.

ENZYME AND MICROBIAL TECHNOLOGY (2017)

Add to Collection
Article Energy & Fuels

Evaluation of different lipase biocatalysts in the production of biodiesel from used cooking oil: Critical role of the immobilization support

Veymar G. Tacias-Pascacio et al.

FUEL (2017)

Add to Collection
Article Biotechnology & Applied Microbiology

Production of glucosyl glycerol by immobilized sucrose phosphorylase: Options for enzyme fixation on a solid support and application in microscale flow format

Juan M. Bolivar et al.

JOURNAL OF BIOTECHNOLOGY (2017)

Add to Collection
Article Chemistry, Multidisciplinary

Variable Lysozyme Transport Dynamics on Oxidatively Functionalized Polystyrene Films

Nicholas A. Moringo et al.

LANGMUIR (2017)

Add to Collection
Article Biochemistry & Molecular Biology

Desorption of Lipases Immobilized on Octyl-Agarose Beads and Coated with Ionic Polymers after Thermal Inactivation. Stronger Adsorption of Polymers/Unfolded Protein Composites

Jose J. Virgen-Ortiz et al.

MOLECULES (2017)

Add to Collection
Article Biochemistry & Molecular Biology

Physical crosslinking of lipase from Rhizomucor miehei immobilized on octyl agarose via coating with ionic polymers Avoiding enzyme release from the support

Laura Fernandez-Lopez et al.

PROCESS BIOCHEMISTRY (2017)

Add to Collection
Article Biochemistry & Molecular Biology

Stabilization of ficin extract by immobilization on glyoxyl agarose. Preliminary characterization of the biocatalyst performance in hydrolysis of proteins

El-Hocine Siar et al.

PROCESS BIOCHEMISTRY (2017)

Add to Collection
Article Biochemistry & Molecular Biology

Effect of immobilization rate and enzyme crowding on enzyme stability under different conditions. The case of lipase from Thermomyces lanuginosus immobilized on octyl agarose beads

Hadjer Zaak et al.

PROCESS BIOCHEMISTRY (2017)

Add to Collection
Article Chemistry, Physical

Toward Rational Design of High-efficiency Enzyme Cascades

Yifei Zhang et al.

ACS CATALYSIS (2017)

Add to Collection
Article Materials Science, Biomaterials

Understanding the functional properties of bio-inorganic nanoflowers as biocatalysts by deciphering the metal-binding sites of enzymes

Sindy Escobar et al.

JOURNAL OF MATERIALS CHEMISTRY B (2017)

Add to Collection
Review Materials Science, Biomaterials

Polyethylenimine: a very useful ionic polymer in the design of immobilized enzyme biocatalysts

Jose J. Virgen-Ortiz et al.

JOURNAL OF MATERIALS CHEMISTRY B (2017)

Add to Collection
Article Chemistry, Physical

Exploiting the Versatility of Aminated Supports Activated with Glutaraldehyde to Immobilize β-galactosidase from Aspergillus oryzae

Hadjer Zaak et al.

CATALYSTS (2017)

Add to Collection
Review Chemistry, Physical

Immobilization of cellulase enzyme onto magnetic nanoparticles: Applications and recent advances

Kamyar Khoshnevisan et al.

MOLECULAR CATALYSIS (2017)

Add to Collection
Article Biochemistry & Molecular Biology

Reversible covalent enzyme immobilization methods for reuse of carriers

Regina Fraas et al.

BIOCATALYSIS AND BIOTRANSFORMATION (2017)

Add to Collection
Article Chemistry, Multidisciplinary

Asymmetric Reduction of Prochiral Ketones by Using Self-Sufficient Heterogeneous Biocatalysts Based on NADPH-Dependent Ketoreductases

Ana I. Benitez-Mateos et al.

CHEMISTRY-A EUROPEAN JOURNAL (2017)

Add to Collection
Article Biochemistry & Molecular Biology

Urease Inhibition in the Presence of N-(n-Butyl)thiophosphoric Triamide, a Suicide Substrate: Structure and Kinetics

Luca Mazzei et al.

BIOCHEMISTRY (2017)

Add to Collection
Review Biochemistry & Molecular Biology

Engineering genetically-encoded tools for optogenetic control of protein activity

Qi Liu et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2017)

Add to Collection
Article Computer Science, Interdisciplinary Applications

Bioprocess intensification for the effective production of chemical products

John M. Woodley

COMPUTERS & CHEMICAL ENGINEERING (2017)

Add to Collection
Review Biochemistry & Molecular Biology

Role of glycosylation in nucleating protein folding and stability

Nisha Grandhi Jayaprakash et al.

BIOCHEMICAL JOURNAL (2017)

Add to Collection
Review Chemistry, Multidisciplinary

Enzyme-MOF (metal-organic framework) composites

Xizhen Lian et al.

CHEMICAL SOCIETY REVIEWS (2017)

Add to Collection
Review Chemistry, Multidisciplinary

Metal-organic and covalent organic frameworks as single-site catalysts

S. M. J. Rogge et al.

CHEMICAL SOCIETY REVIEWS (2017)

Add to Collection
Review Biochemistry & Molecular Biology

Computational tools for enzyme improvement: why everyone can - and should - use them

Maximilian C. C. J. C. Ebert et al.

CURRENT OPINION IN CHEMICAL BIOLOGY (2017)

Add to Collection
Review Chemistry, Multidisciplinary

Biocatalysis engineering: the big picture

Roger A. Sheldon et al.

CHEMICAL SOCIETY REVIEWS (2017)

Add to Collection
Article Agricultural Engineering

Microenvironmental pH changes in immobilized cephalosporin C acylase during a proton-producing reaction and regulation by a two-stage catalytic process

Hui Luo et al.

BIORESOURCE TECHNOLOGY (2017)

Add to Collection
Article Chemistry, Multidisciplinary

Surface-Mediated Protein Unfolding as a Search Process for Denaturing Sites

James S. Weltz et al.

ACS NANO (2016)

Add to Collection
Article Biotechnology & Applied Microbiology

Scale-Up of Industrial Biodiesel Production to 40 m3 Using a Liquid Lipase Formulation

Jason Price et al.

BIOTECHNOLOGY AND BIOENGINEERING (2016)

Add to Collection
Article Biotechnology & Applied Microbiology

Let the Substrate Flow, Not the Enzyme: Practical Immobilization of D-Amino Acid Oxidase in a Glass Microreactor for Effective Biocatalytic Conversions

Juan M. Bolivar et al.

BIOTECHNOLOGY AND BIOENGINEERING (2016)

Add to Collection
Article Chemistry, Applied

Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

Juan M. Bolivar et al.

CATALYSIS TODAY (2016)

Add to Collection
Article Engineering, Environmental

Preparation of lipase/Zn3(PO4)2 hybrid nanoflower and its catalytic performance as an immobilized enzyme

Baoliang Zhang et al.

CHEMICAL ENGINEERING JOURNAL (2016)

Add to Collection
Article Chemistry, Multidisciplinary

Chemical Modification in the Design of Immobilized Enzyme Biocatalysts: Drawbacks and Opportunities

Nazzoly Rueda et al.

CHEMICAL RECORD (2016)

Add to Collection
Review Chemistry, Multidisciplinary

Mild metal-catalyzed C-H activation: examples and concepts

T. Gensch et al.

CHEMICAL SOCIETY REVIEWS (2016)

Add to Collection
Review Chemistry, Inorganic & Nuclear

Recent advances in enzyme immobilization techniques: Metal-organic frameworks as novel substrates

Jyotsana Mehta et al.

COORDINATION CHEMISTRY REVIEWS (2016)

Add to Collection
Article Biochemistry & Molecular Biology

Intensifying the O2-dependent heterogeneous biocatalysis: Superoxygenation of solid support from H2O2 by a catalase tailor-made for effective immobilization

Juan M. Bolivar et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2016)

Add to Collection
Article Biochemistry & Molecular Biology

Evaluation of different immobilized lipases in transesterification reactions using tributyrin: Advantages of the heterofunctional octyl agarose beads

Daniela B. Hirata et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2016)

Add to Collection
Article Biochemistry & Molecular Biology

Reversible immobilization of lipases on octyl-glutamic agarose beads: A mixed adsorption that reinforces enzyme immobilization

Nazzoly Ruedaa et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2016)

Add to Collection
Article Chemistry, Medicinal

Effect of Molecular Structure on the Relative Hydrogen Peroxide Scavenging Ability of Some α-Keto Carboxylic Acids

Antonio Lopalco et al.

JOURNAL OF PHARMACEUTICAL SCIENCES (2016)

Add to Collection
Article Chemistry, Applied

Production of Biodiesel Using Liquid Lipase Formulations

P. M. Nielsen et al.

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY (2016)

Add to Collection
Article Biochemistry & Molecular Biology

Reversible Immobilization of Lipases on Heterofunctional Octyl-Amino Agarose Beads Prevents Enzyme Desorption

Nazzoly Rueda et al.

MOLECULES (2016)

Add to Collection
Review Biochemistry & Molecular Biology

Agarose and Its Derivatives as Supports for Enzyme Immobilization

Paolo Zucca et al.

MOLECULES (2016)

Add to Collection
Article Biochemistry & Molecular Biology

Reuse of anion exchangers as supports for enzyme immobilization: Reinforcement of the enzyme-support multiinteraction after enzyme inactivation

Jose J. Virgen-Ortiz et al.

PROCESS BIOCHEMISTRY (2016)

Add to Collection
Article Biochemistry & Molecular Biology

Easy stabilization of interfacially activated lipases using heterofunctional divinyl sulfone activated-octyl agarose beads. Modulation of the immobilized enzymes by altering their nanoenvironment

Tiago L. de Albuquerque et al.

PROCESS BIOCHEMISTRY (2016)

Add to Collection
Review Biophysics

Modeling and simulation of protein-surface interactions: achievements and challenges

Musa Ozboyaci et al.

QUARTERLY REVIEWS OF BIOPHYSICS (2016)

Add to Collection
Article Chemistry, Physical

Rational Enzyme Engineering Through Biophysical and Biochemical Modeling

Sandra Acebes et al.

ACS CATALYSIS (2016)

Add to Collection
Article Chemistry, Multidisciplinary

Development of simple protocols to solve the problems of enzyme coimmobilization. Application to coimmobilize a lipase and a beta-galactosidase

Sara Peirce et al.

RSC ADVANCES (2016)

Add to Collection
Article Chemistry, Multidisciplinary

Advantages of Heterofunctional Octyl Supports: Production of 1,2-Dibutyrin by Specific and Selective Hydrolysis of Tributyrin Catalyzed by Immobilized Lipases

Daniela B. Hirata et al.

CHEMISTRYSELECT (2016)

Add to Collection
Article Chemistry, Multidisciplinary

Evaluation of different commercial hydrophobic supports for the immobilization of lipases: tuning their stability, activity and specificity

Veymar G. Tacias-Pascacio et al.

RSC ADVANCES (2016)

Add to Collection
Review Chemistry, Multidisciplinary

Nanomaterials for biocatalyst immobilization - state of the art and future trends

Eliane P. Cipolatti et al.

RSC ADVANCES (2016)

Add to Collection
Article Chemistry, Multidisciplinary

Large scale applications of immobilized enzymes call for sustainable and inexpensive solutions: rice husks as renewable alternatives to fossil-based organic resins

L. Corici et al.

RSC ADVANCES (2016)

Add to Collection
Review Biotechnology & Applied Microbiology

An overview of technologies for immobilization of enzymes and surface analysis techniques for immobilized enzymes

Nur Royhaila Mohamad et al.

BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT (2015)

Add to Collection
Review Biotechnology & Applied Microbiology

Strategies for the one-step immobilization-purification of enzymes as industrial biocatalysts

Oveimar Barbosa et al.

BIOTECHNOLOGY ADVANCES (2015)

Add to Collection
Article Chemistry, Applied

Asymmetric hydrolysis of dimethyl-3-phenylglutarate in sequential batch reactor operation catalyzed by immobilized Geobacillus thermocatenulatus lipase

Nadia Guajardo et al.

CATALYSIS TODAY (2015)

Add to Collection
Review Chemistry, Physical

Importance of the Support Properties for Immobilization or Purification of Enzymes

Jose Cleiton S. dos Santos et al.

CHEMCATCHEM (2015)

Add to Collection
Article Biotechnology & Applied Microbiology

Immobilization of lipases on hydrophobic supports involves the open form of the enzyme

Evelin A. Manoel et al.

ENZYME AND MICROBIAL TECHNOLOGY (2015)

Add to Collection
Article Biotechnology & Applied Microbiology

Tuning the catalytic properties of lipases immobilized on divinylsulfone activated agarose by altering its nanoenvironment

Jose C. S. dos Santos et al.

ENZYME AND MICROBIAL TECHNOLOGY (2015)

Add to Collection
Article Engineering, Chemical

Synthesis of Structured Lipids by Enzymatic Interesterification of Milkfat and Soybean Oil in a Basket-Type Stirred Tank Reactor

Ariela V. Paula et al.

INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH (2015)

Add to Collection
Article Agriculture, Multidisciplinary

Improvement of Efficiency in the Enzymatic Synthesis of Lactulose Palmitate

Claudia Bernal et al.

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY (2015)

Add to Collection
Article Biochemistry & Molecular Biology

Evaluation of divinylsulfone activated agarose to immobilize lipases and to tune their catalytic properties

Jose C. S. dos Santos et al.

PROCESS BIOCHEMISTRY (2015)

Add to Collection
Article Chemistry, Analytical

Degradation of phenolic compounds by laccase immobilized on carbon nanomaterials: Diffusional limitation investigation

Ran Pang et al.

TALANTA (2015)

Add to Collection
Review Biotechnology & Applied Microbiology

Microscale technology and biocatalytic processes: opportunities and challenges for synthesis

Roland Wohlgemuth et al.

TRENDS IN BIOTECHNOLOGY (2015)

Add to Collection
Article Acoustics

The combined use of ultrasound and molecular sieves improves the synthesis of ethyl butyrate catalyzed by immobilized Thermomyces lanuginosus lipase

Natalia Paludo et al.

ULTRASONICS SONOCHEMISTRY (2015)

Add to Collection
Article Chemistry, Multidisciplinary

Novel artificial metalloenzymes by in vivo incorporation of metal-binding unnatural amino acids

Ivana Drienovska et al.

CHEMICAL SCIENCE (2015)

Add to Collection
Article Chemistry, Physical

Mesoporous Silica Materials Labeled for Optical Oxygen Sensing and Their Application to Development of a Silica-Supported Oxidoreductase Biocatalyst

Juan M. Bolivar et al.

ACS CATALYSIS (2015)

Add to Collection
Review Chemistry, Physical

Metal-organic frameworks and inorganic nanoflowers: a type of emerging inorganic crystal nanocarrier for enzyme immobilization

Xiaoling Wu et al.

CATALYSIS SCIENCE & TECHNOLOGY (2015)

Add to Collection
Article Chemistry, Multidisciplinary

Versatility of divinylsulfone supports permits the tuning of CALB properties during its immobilization

Jose C. S. dos Santos et al.

RSC ADVANCES (2015)

Add to Collection
Article Chemistry, Multidisciplinary

Characterization of supports activated with divinyl sulfone as a tool to immobilize and stabilize enzymes via multipoint covalent attachment. Application to chymotrypsin

Jose C. S. dos Santos et al.

RSC ADVANCES (2015)

Add to Collection
Article Multidisciplinary Sciences

Mesoporous CLEAs-silica composite microparticles with high activity and enhanced stability

Jiandong Cui et al.

SCIENTIFIC REPORTS (2015)

Add to Collection
Article Materials Science, Biomaterials

An enzyme-inorganic hybrid nanoflower based immobilized enzyme reactor with enhanced enzymatic activity

Yuqing Yin et al.

JOURNAL OF MATERIALS CHEMISTRY B (2015)

Add to Collection
Article Biochemistry & Molecular Biology

Reversible covalent immobilization of Trametes villosa laccase onto thiolsulfinate-agarose: An insoluble biocatalyst with potential for decoloring recalcitrant dyes

Larissa Gioia et al.

BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY (2015)

Add to Collection
Article Chemistry, Multidisciplinary

Improved performance of lipases immobilized on heterofunctional octyl-glyoxyl agarose beads

Nazzoly Rueda et al.

RSC ADVANCES (2015)

Add to Collection
Review Chemistry, Physical

Enzyme immobilization by adsorption: a review

Teofil Jesionowski et al.

ADSORPTION-JOURNAL OF THE INTERNATIONAL ADSORPTION SOCIETY (2014)

Add to Collection
Article Chemistry, Physical

A bottom-up approach to understanding protein layer formation at solid-liquid interfaces

Mark Kastantin et al.

ADVANCES IN COLLOID AND INTERFACE SCIENCE (2014)

Add to Collection
Article Biochemical Research Methods

Microscopic monitoring provides information on structure and properties during biocatalyst immobilization

Sarka Bidmanova et al.

BIOTECHNOLOGY JOURNAL (2014)

Add to Collection
Article Chemistry, Physical

Dissecting Physical and Biochemical Factors of Catalytic Effectiveness in Immobilized d- Amino Acid Oxidase by Real- Time Sensing of O2 Availability Inside Porous Carriers

Juan M. Bolivar et al.

CHEMCATCHEM (2014)

Add to Collection
Article Polymer Science

Nanocellulose in biomedicine: Current status and future prospect

Ning Lin et al.

EUROPEAN POLYMER JOURNAL (2014)

Add to Collection
Article Biochemistry & Molecular Biology

Evaluation of kinetic parameters of immobilized penicillin G acylase subject to an inactivation and reactivation process

Oscar Romero et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2014)

Add to Collection
Article Chemistry, Multidisciplinary

Heterofunctional Hydrophilic Hydrophobic Porous Silica as Support for Multipoint Covalent Immobilization of Lipases: Application to Lactulose Palmitate Synthesis

Claudia Bernal et al.

LANGMUIR (2014)

Add to Collection
Review Chemistry, Multidisciplinary

Increasing the activity and enantioselectivity of lipases by sol-gel immobilization: further advancements of practical interest

Patrick Tielmann et al.

NANOSCALE (2014)

Add to Collection
Article Biochemistry & Molecular Biology

Improvement of the stability and selectivity of Rhizomucor miehei lipase immobilized on silica nanoparticles: Selective hydrolysis of fish oil using immobilized preparations

Mehdi Mohammadi et al.

PROCESS BIOCHEMISTRY (2014)

Add to Collection
Review Thermodynamics

Ultrasound-enhanced conversion of biomass to biofuels

Jia Luo et al.

PROGRESS IN ENERGY AND COMBUSTION SCIENCE (2014)

Add to Collection
Review Chemistry, Multidisciplinary

Glutaraldehyde in bio-catalysts design: a useful crosslinker and a versatile tool in enzyme immobilization

Oveimar Barbosa et al.

RSC ADVANCES (2014)

Add to Collection
Article Chemistry, Multidisciplinary

Ultrasound technology and molecular sieves improve the thermodynamically controlled esterification of butyric acid mediated by immobilized lipase from Rhizomucor miehei

Lucas P. Fallavena et al.

RSC ADVANCES (2014)

Add to Collection
Review Chemistry, Multidisciplinary

Amination of enzymes to improve biocatalyst performance: coupling genetic modification and physicochemical tools

Rafael C. Rodrigues et al.

RSC ADVANCES (2014)

Add to Collection
Article Pharmacology & Pharmacy

'Genipin' - The Natural Water Soluble Cross- linking Agent and Its Importance in the Modified Drug Delivery Systems: An Overview

Balamurugan Manickam et al.

CURRENT DRUG DELIVERY (2014)

Add to Collection
Review Biochemistry & Molecular Biology

Heterofunctional Supports in Enzyme Immobilization: From Traditional Immobilization Protocols to Opportunities in Tuning Enzyme Properties

Oveimar Barbosa et al.

BIOMACROMOLECULES (2013)

Add to Collection
Article Agricultural Engineering

Improved production of butyl butyrate with lipase from Thermomyces lanuginosus immobilized on styrene-divinylbenzene beads

Andrea B. Martins et al.

BIORESOURCE TECHNOLOGY (2013)

Add to Collection
Article Biotechnology & Applied Microbiology

Quantitating intraparticle O2 gradients in solid supported enzyme immobilizates: Experimental determination of their role in limiting the catalytic effectiveness of immobilized glucose oxidase

Juan M. Bolivar et al.

BIOTECHNOLOGY AND BIOENGINEERING (2013)

Add to Collection
Article Chemistry, Applied

Investigation of the stability of Novozym® 435 in the production of biodiesel

Carla Jose et al.

CATALYSIS TODAY (2013)

Add to Collection
Review Chemistry, Multidisciplinary

Enzyme immobilisation in biocatalysis: why, what and how

Roger A. Sheldon et al.

CHEMICAL SOCIETY REVIEWS (2013)

Add to Collection
Review Chemistry, Multidisciplinary

Industrial use of immobilized enzymes

Robert DiCosimo et al.

CHEMICAL SOCIETY REVIEWS (2013)

Add to Collection
Review Chemistry, Multidisciplinary

Efficient immobilisation of industrial biocatalysts: criteria and constraints for the selection of organic polymeric carriers and immobilisation methods

Sara Cantone et al.

CHEMICAL SOCIETY REVIEWS (2013)

Add to Collection
Review Chemistry, Multidisciplinary

Modifying enzyme activity and selectivity by immobilization

Rafael C. Rodrigues et al.

CHEMICAL SOCIETY REVIEWS (2013)

Add to Collection
Review Chemistry, Multidisciplinary

Immobilised lipases in the cosmetics industry

Marion B. Ansorge-Schumacher et al.

CHEMICAL SOCIETY REVIEWS (2013)

Add to Collection
Review Chemistry, Multidisciplinary

Evaluation of immobilized enzymes for industrial applications

Andreas Liese et al.

CHEMICAL SOCIETY REVIEWS (2013)

Add to Collection
Review Chemistry, Multidisciplinary

Conformational changes of enzymes upon immobilisation

Francesco Secundo

CHEMICAL SOCIETY REVIEWS (2013)

Add to Collection
Review Chemistry, Multidisciplinary

Stabilizing biocatalysts

Andreas S. Bommarius et al.

CHEMICAL SOCIETY REVIEWS (2013)

Add to Collection
Article Environmental Sciences

Cross-linked tyrosinase aggregates for elimination of phenolic compounds from wastewater

Da-You Xu et al.

CHEMOSPHERE (2013)

Add to Collection
Review Biotechnology & Applied Microbiology

Lipase applications in oil hydrolysis with a case study on castor oil: a review

Debajyoti Goswami et al.

CRITICAL REVIEWS IN BIOTECHNOLOGY (2013)

Add to Collection
Review Biochemistry & Molecular Biology

Protein engineering of enzymes for process applications

John M. Woodley

CURRENT OPINION IN CHEMICAL BIOLOGY (2013)

Add to Collection
Article Biotechnology & Applied Microbiology

Stabilization of the hexameric glutamate dehydrogenase from Escherichia coli by cations and polyethyleneimine

Cristina Garcia-Galan et al.

ENZYME AND MICROBIAL TECHNOLOGY (2013)

Add to Collection
Article Biotechnology & Applied Microbiology

Site directed immobilization of glucose-6-phosphate dehydrogenase via thiol-disulfide interchange: Influence on catalytic activity of cysteines introduced at different positions

J. R. Simons et al.

JOURNAL OF BIOTECHNOLOGY (2013)

Add to Collection
Article Biotechnology & Applied Microbiology

Effect of immobilization protocol on optimal conditions of ethyl butyrate synthesis catalyzed by lipase B from Candida antarctica

John L. R. Friedrich et al.

JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY (2013)

Add to Collection
Article Biochemistry & Molecular Biology

Optimization of synthesis of fatty acid methyl esters catalyzed by lipase B from Candida antarctica immobilized on hydrophobic supports

Jakeline K. Poppe et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2013)

Add to Collection
Article Biochemistry & Molecular Biology

Modulation of the Microenvironment Surrounding the Active Site of Penicillin G Acylase Immobilized on Acrylic Carriers Improves the Enzymatic Synthesis of Cephalosporins

Paolo Bonomi et al.

MOLECULES (2013)

Add to Collection
Article Multidisciplinary Sciences

Immobilization of Cross-Linked Phenylalanine Ammonia Lyase Aggregates in Microporous Silica Gel

Jian Dong Cui et al.

PLOS ONE (2013)

Add to Collection
Article Biochemistry & Molecular Biology

Real-time measurement and modeling of intraparticle pH gradient formation in immobilized cephalosporin C amidase

Thomas Zahel et al.

PROCESS BIOCHEMISTRY (2013)

Add to Collection
Article Chemistry, Inorganic & Nuclear

The combi-CLEA approach: enzymatic cascade synthesis of enantiomerically pure (S)-mandelic acid

Andrzej Chmura et al.

TETRAHEDRON-ASYMMETRY (2013)

Add to Collection
Review Biotechnology & Applied Microbiology

Shine a light on immobilized enzymes: real-time sensing in solid supported biocatalysts

Juan M. Bolivar et al.

TRENDS IN BIOTECHNOLOGY (2013)

Add to Collection
Article Acoustics

Ultrasound-assisted butyl acetate synthesis catalyzed by Novozym 435: Enhanced activity and operational stability

Andrea B. Martins et al.

ULTRASONICS SONOCHEMISTRY (2013)

Add to Collection
Article Biotechnology & Applied Microbiology

Use of cross-linked tyrosinase aggregates as catalyst for synthesis of L-DOPA

Da-You Xu et al.

BIOCHEMICAL ENGINEERING JOURNAL (2012)

Add to Collection
Article Biotechnology & Applied Microbiology

6-Aminopenicillanic acid production in stationary basket bioreactor with packed bed of immobilized penicillin amidase-Penicillin G mass transfer and consumption rate under internal diffusion limitation

Dan Cascaval et al.

BIOCHEMICAL ENGINEERING JOURNAL (2012)

Add to Collection
Article Biochemical Research Methods

Directed, Strong, and Reversible Immobilization of Proteins Tagged with a β-Trefoil Lectin Domain: A Simple Method to Immobilize Biomolecules on Plain Agarose Matrixes

Fernando Lopez-Gallego et al.

BIOCONJUGATE CHEMISTRY (2012)

Add to Collection
Article Biotechnology & Applied Microbiology

A robust methodology for kinetic model parameter estimation for biocatalytic reactions

Naweed Al-Haque et al.

BIOTECHNOLOGY PROGRESS (2012)

Add to Collection
Article Biotechnology & Applied Microbiology

Immobilization of lipase B from Candida antarctica on porous styrene-divinylbenzene beads improves butyl acetate synthesis

Natalia G. Graebin et al.

BIOTECHNOLOGY PROGRESS (2012)

Add to Collection
Article Biotechnology & Applied Microbiology

Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts

Caterina Boniello et al.

BMC BIOTECHNOLOGY (2012)

Add to Collection
Article Chemistry, Physical

Rational Co-Immobilization of Bi-Enzyme Cascades on Porous Supports and their Applications in Bio-Redox Reactions with In Situ Recycling of Soluble Cofactors

Javier Rocha-Martin et al.

CHEMCATCHEM (2012)

Add to Collection
Review Chemistry, Multidisciplinary

Production of Hydrogen from Ethanol: Review of Reaction Mechanism and Catalyst Deactivation

Lisiane V. Mattos et al.

CHEMICAL REVIEWS (2012)

Add to Collection
Review Chemistry, Multidisciplinary

Aqueous biphasic systems: a boost brought about by using ionic liquids

Mara G. Freire et al.

CHEMICAL SOCIETY REVIEWS (2012)

Add to Collection
Review Chemistry, Physical

Tailoring Sol-Gel-Derived Silica Materials for Optical Biosensing

Maria Rowena N. Monton et al.

CHEMISTRY OF MATERIALS (2012)

Add to Collection
Article Chemistry, Organic

Hydrogen Peroxide in Biocatalysis. A Dangerous Liaison

Karel Hernandez et al.

CURRENT ORGANIC CHEMISTRY (2012)

Add to Collection
Article Chemistry, Organic

CAL-B-Catalyzed Acylation of Nucleosides and Role of the Sugar Conformation: An Improved Understanding of the Enzyme-Substrate Recognition

Saul Martinez-Montero et al.

EUROPEAN JOURNAL OF ORGANIC CHEMISTRY (2012)

Add to Collection
Article Biochemistry & Molecular Biology

Influence of different immobilization techniques for Candida cylindracea lipase on its stability and fish oil hydrolysis

Carolina Pizarro et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2012)

Add to Collection
Article Multidisciplinary Sciences

Stabilizing Additives Added during Cell Lysis Aid in the Solubilization of Recombinant Proteins

David J. Leibly et al.

PLOS ONE (2012)

Add to Collection
Article Biochemistry & Molecular Biology

Modulation of the properties of immobilized CALB by chemical modification with 2,3,4-trinitrobenzenesulfonate or ethylendiamine. Advantages of using adsorbed lipases on hydrophobic supports

Oveimar Barbosa et al.

PROCESS BIOCHEMISTRY (2012)

Add to Collection
Article Biochemistry & Molecular Biology

Biocatalytic acylation of sugar alcohols by 3-(4-hydroxyphenyl)propionic acid

R. Croitoru et al.

PROCESS BIOCHEMISTRY (2012)

Add to Collection
Article Biochemistry & Molecular Biology

Versatility of glutaraldehyde to immobilize lipases: Effect of the immobilization protocol on the properties of lipase B from Candida antarctica

Oveimar Barbosa et al.

PROCESS BIOCHEMISTRY (2012)

Add to Collection
Article Biochemistry & Molecular Biology

The slow-down of the CALB immobilization rate permits to control the inter and intra molecular modification produced by glutaraldehyde

Oveimar Barbosa et al.

PROCESS BIOCHEMISTRY (2012)

Add to Collection
Review Food Science & Technology

Food related applications of magnetic iron oxide nanoparticles: Enzyme immobilization, protein purification, and food analysis

Miao Cao et al.

TRENDS IN FOOD SCIENCE & TECHNOLOGY (2012)

Add to Collection
Review Chemistry, Applied

Coupling Chemical Modification and Immobilization to Improve the Catalytic Performance of Enzymes

Rafael C. Rodrigues et al.

ADVANCED SYNTHESIS & CATALYSIS (2011)

Add to Collection
Review Chemistry, Applied

Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance

Cristina Garcia-Galan et al.

ADVANCED SYNTHESIS & CATALYSIS (2011)

Add to Collection
Review Biotechnology & Applied Microbiology

Characteristic features and biotechnological applications of cross-linked enzyme aggregates (CLEAs)

Roger A. Sheldon

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY (2011)

Add to Collection
Article Biotechnology & Applied Microbiology

Selection of CalB immobilization method to be used in continuous oil transesterification: Analysis of the economical impact

Etienne Severac et al.

ENZYME AND MICROBIAL TECHNOLOGY (2011)

Add to Collection
Review Biotechnology & Applied Microbiology

Enhancing the functional properties of thermophilic enzymes by chemical modification and immobilization

Don A. Cowan et al.

ENZYME AND MICROBIAL TECHNOLOGY (2011)

Add to Collection
Review Biotechnology & Applied Microbiology

Control of protein immobilization: Coupling immobilization and site-directed mutagenesis to improve biocatalyst or biosensor performance

Karel Hernandez et al.

ENZYME AND MICROBIAL TECHNOLOGY (2011)

Add to Collection
Article Biotechnology & Applied Microbiology

Modulation of the distribution of small proteins within porous matrixes by smart-control of the immobilization rate

Juan M. Bolivar et al.

JOURNAL OF BIOTECHNOLOGY (2011)

Add to Collection
Article Biochemistry & Molecular Biology

Reactivation of immobilized penicillin G acylase: Influence of cosolvents and catalytic modulators

Valeria Miranda et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2011)

Add to Collection
Article Biochemistry & Molecular Biology

Investigation of the causes of deactivation-degradation of the commercial biocatalyst Novozym® 435 in ethanol and ethanol-aqueous media

Carla Jose et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2011)

Add to Collection
Article Chemistry, Applied

Cross-Linking of Lipases Adsorbed on Hydrophobic Supports: Highly Selective Hydrolysis of Fish Oil Catalyzed by RML

Gloria Fernandez-Lorente et al.

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY (2011)

Add to Collection
Review Chemistry, Applied

Cross-Linked Enzyme Aggregates as Industrial Biocatalysts

Roger A. Sheldon

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2011)

Add to Collection
Article Chemistry, Applied

Guidelines and Cost Analysis for Catalyst Production in Biocatalytic Processes

Par Tufvesson et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2011)

Add to Collection
Article Biochemistry & Molecular Biology

Lipase B from Candida antarctica immobilized on octadecyl Sepabeads: A very stable biocatalyst in the presence of hydrogen peroxide

Karel Hernandez et al.

PROCESS BIOCHEMISTRY (2011)

Add to Collection
Review Biotechnology & Applied Microbiology

Biotransformations in microstructured reactors: more than flowing with the stream?

Juan M. Bolivar et al.

TRENDS IN BIOTECHNOLOGY (2011)

Add to Collection
Article Biotechnology & Applied Microbiology

Magnetic polymeric nanospheres as an immobilized metal affinity chromatography (IMAC) support for catalase

M. Emin Corman et al.

BIOCHEMICAL ENGINEERING JOURNAL (2010)

Add to Collection
Article Biochemistry & Molecular Biology

Improvement of Enzyme Properties with a Two-Step Immobilizaton Process on Novel Heterofunctional Supports

Cesar Mateo et al.

BIOMACROMOLECULES (2010)

Add to Collection
Article Biotechnology & Applied Microbiology

Intraparticle Concentration Gradients for Substrate and Acidic Product in Immobilized Cephalosporin C Amidase and Their Dependencies on Carrier Characteristics and Reaction Parameters

Caterina Boniello et al.

BIOTECHNOLOGY AND BIOENGINEERING (2010)

Add to Collection
Article Chemistry, Applied

Fundamental understanding of deactivation and regeneration of cobalt Fischer-Tropsch synthesis catalysts

A. M. Saib et al.

CATALYSIS TODAY (2010)

Add to Collection
Review Biochemistry & Molecular Biology

Use of Enzymes in the Production of Semi-Synthetic Penicillins and Cephalosporins: Drawbacks and Perspectives

G. Volpato et al.

CURRENT MEDICINAL CHEMISTRY (2010)

Add to Collection
Article Biotechnology & Applied Microbiology

Process considerations for the scale-up and implementation of biocatalysis

Par Tufvesson et al.

FOOD AND BIOPRODUCTS PROCESSING (2010)

Add to Collection
Article Biochemistry & Molecular Biology

Partition and substrate concentration effect in the enzymatic synthesis of cephalexin in aqueous two-phase systems

Carolina Aguirre et al.

PROCESS BIOCHEMISTRY (2010)

Add to Collection
Article Biochemistry & Molecular Biology

One-step purification and immobilization of His-tagged rhamnosidase for naringin hydrolysis

Munish Puri et al.

PROCESS BIOCHEMISTRY (2010)

Add to Collection
Article Biochemistry & Molecular Biology

Promotion of multipoint covalent immobilization through different regions of genetically modified penicillin G acylase from E. coli

Valeria Grazu et al.

PROCESS BIOCHEMISTRY (2010)

Add to Collection
Article Chemistry, Applied

Synthesis of Aliphatic (S)-α-Hydroxycarboxylic Amides using a One-Pot Bienzymatic Cascade of Immobilised Oxynitrilase and Nitrile Hydratase

Sander van Pelt et al.

ADVANCED SYNTHESIS & CATALYSIS (2009)

Add to Collection
Review Chemistry, Analytical

Integration of Biomolecules into Analytical Systems by Means of Silica Sol-Gel Technology

Kumiko Sakai-Kato et al.

ANALYTICAL SCIENCES (2009)

Add to Collection
Review Chemistry, Multidisciplinary

Metal-Organic Frameworks: Opportunities for Catalysis

David Farrusseng et al.

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2009)

Add to Collection
Article Biochemistry & Molecular Biology

Coating of Soluble and Immobilized Enzymes with Ionic Polymers: Full Stabilization of the Quaternary Structure of Multimeric Enzymes

Juan M. Bolivar et al.

BIOMACROMOLECULES (2009)

Add to Collection
Article Biotechnology & Applied Microbiology

Utilization of Cross-Linked Laccase Aggregates in a Perfusion Basket Reactor for the Continuous Elimination of Endocrine-Disrupting Chemicals

Hubert Cabana et al.

BIOTECHNOLOGY AND BIOENGINEERING (2009)

Add to Collection
Article Biochemistry & Molecular Biology

Multiple Pathways for the Irreversible Inhibition of Steroid Sulfatase with Quinone Methide-Generating Suicide Inhibitors

Vanessa Ahmed et al.

CHEMBIOCHEM (2009)

Add to Collection
Article Engineering, Chemical

Effect of pore diffusional resistance on biocatalytic activity of Burkholderia cepacia lipase immobilized on SBA-15 hosts

Hemachand Jaladi et al.

CHEMICAL ENGINEERING SCIENCE (2009)

Add to Collection
Review Chemistry, Multidisciplinary

Understanding enzyme immobilisation

Ulf Hanefeld et al.

CHEMICAL SOCIETY REVIEWS (2009)

Add to Collection
Review Biotechnology & Applied Microbiology

Stabilization of multimeric enzymes: Strategies to prevent subunit dissociation

Roberto Fernandez-Lafuente

ENZYME AND MICROBIAL TECHNOLOGY (2009)

Add to Collection
Article Biochemistry & Molecular Biology

Enhancement of the activity and enantioselectivity of lipase in organic systems by immobilization onto low-cost support

Guang Yang et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2009)

Add to Collection
Article Biochemistry & Molecular Biology

Purification and stabilization of a glutamate dehygrogenase from Thermus thermophilus via oriented multisubunit plus multipoint covalent immobilization

Juan M. Bolivar et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2009)

Add to Collection
Article Biochemistry & Molecular Biology

Immobilization of antibodies through the surface regions having the highest density in lysine groups on finally inert support surfaces

Pilar Batalla et al.

PROCESS BIOCHEMISTRY (2009)

Add to Collection
Review Biotechnology & Applied Microbiology

Low-frequency ultrasound in biotechnology: state of the art

Ekaterina V. Rokhina et al.

TRENDS IN BIOTECHNOLOGY (2009)

Add to Collection
Article Biochemistry & Molecular Biology

Covalent immobilization of antibodies on finally inert support surfaces through their surface regions having the highest densities in carboxyl groups

Pilar Batalla et al.

BIOMACROMOLECULES (2008)

Add to Collection
Article Biochemistry & Molecular Biology

Oriented covalent immobilization of antibodies on physically inert and hydrophilic support surfaces through their glycosidic chains

Pilar Batalla et al.

BIOMACROMOLECULES (2008)

Add to Collection
Article Biochemistry & Molecular Biology

Solid-phase chemical amination of a lipase from Bacillus thermocatenulatus to improve its stabilization via covalent immobilization on highly activated glyoxyl-agarose

Gloria Fernandez-Lorente et al.

BIOMACROMOLECULES (2008)

Add to Collection
Review Engineering, Chemical

Extraction and purification of bioproducts and nanoparticles using Aqueous Two-Phase Systems strategies

Jorge Benavides et al.

CHEMICAL ENGINEERING & TECHNOLOGY (2008)

Add to Collection
Article Biochemistry & Molecular Biology

The influence of the support nature on the kinetics parameters, inhibition constants and reactivation of immobilized acetylcholinesterase

Katya Gabrovska et al.

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES (2008)

Add to Collection
Article Engineering, Environmental

Combined effects of external mass transfer and biodegradation rates on removal of phenol by immobilized Ralstonia eutropha in a packed bed reactor

Ozlem Tepe et al.

JOURNAL OF HAZARDOUS MATERIALS (2008)

Add to Collection
Article Biochemistry & Molecular Biology

Preparation of an immobilized-stabilized catalase derivative from Aspergillus niger having its multimeric structure stabilized: The effect of Zn2+ on enzyme stability

Samia Kaddour et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2008)

Add to Collection
Article Chemistry, Applied

Efficient immobilization of lecitase in gelatin hydrogel and degumming of rice bran oil using a spinning basket reactor

Gurrala Sheelu et al.

JOURNAL OF THE AMERICAN OIL CHEMISTS SOCIETY (2008)

Add to Collection
Article Chemistry, Multidisciplinary

Dipeptide Synthesis in Biphasic Medium: Evaluating the use of Commercial Porcine Pancreatic Lipase Preparations and the Involvement of Contaminant Proteases

Cleber W. Liria et al.

JOURNAL OF THE BRAZILIAN CHEMICAL SOCIETY (2008)

Add to Collection
Article Biochemistry & Molecular Biology

Interfacially activated lipases against hydrophobic supports: Effect of the support nature on the biocatalytic properties

Gloria Fernandez-Lorente et al.

PROCESS BIOCHEMISTRY (2008)

Add to Collection
Article Biochemistry & Molecular Biology

Cross-linked enzyme aggregates (CLEA®s):: stable and recyclable biocatalysts

R. A. Sheldon

BIOCHEMICAL SOCIETY TRANSACTIONS (2007)

Add to Collection
Article Biochemistry & Molecular Biology

Impressive effect of immobilization conditions on the catalytic activity and enantioselectivity of Candida rugosa lipase toward S-Naproxen production

Serpil Takac et al.

PROCESS BIOCHEMISTRY (2007)

Add to Collection
Review Chemistry, Applied

Enzyme immobilization: The quest for optimum performance

Roger A. Sheldon

ADVANCED SYNTHESIS & CATALYSIS (2007)

Add to Collection
Review Biotechnology & Applied Microbiology

Improvement of enzyme activity, stability and selectivity via immobilization techniques

Cesar Mateo et al.

ENZYME AND MICROBIAL TECHNOLOGY (2007)

Add to Collection
Article Biotechnology & Applied Microbiology

Effect of the support and experimental conditions in the intensity of the multipoint covalent attachment of proteins on glyoxyl-agarose supports:: Correlation between enzyme-support linkages and thermal stability

Justo Pedroche et al.

ENZYME AND MICROBIAL TECHNOLOGY (2007)

Add to Collection
Article Biochemical Research Methods

Immobilization of enzymes on heterofunctional epoxy supports

Cesar Mateo et al.

NATURE PROTOCOLS (2007)

Add to Collection
Review Chemistry, Multidisciplinary

Biofriendly sol-gel processing for the entrapment of soluble and membrane-bound proteins: Toward novel solid-phase assays for high-throughput screening

John D. Brennan

ACCOUNTS OF CHEMICAL RESEARCH (2007)

Add to Collection
Review Biotechnology & Applied Microbiology

Substrate supply for effective biocatalysis

Pei-Yi Kim et al.

BIOTECHNOLOGY PROGRESS (2007)

Add to Collection
Article Biochemical Research Methods

Bioaffinity-based an inexpensive and high yield procedure for the immobilization of turnip (Brassica rapa) peroxidase

Yasha Kulshrestha et al.

BIOMOLECULAR ENGINEERING (2006)

Add to Collection
Article Chemistry, Multidisciplinary

Fabrication of magnetic core-shell CoFe2O4/Al2O3 nanoparticles as immobilized metal chelate affinity support for protein adsorption

Dian Quan Tang et al.

CHEMISTRY LETTERS (2006)

Add to Collection
Article Biochemistry & Molecular Biology

Enantioselectivity modulation through immobilization of Arthrobacter sp lipase:: Kinetic resolution of fluoxetine intermediate

Asha Chaubey et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2006)

Add to Collection
Article Biochemistry & Molecular Biology

The enantioselectivity of Candida rugosa lipase is influenced by the particle size of the immobilising support material Accurel

Sunil Sabbani et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2006)

Add to Collection
Article Biotechnology & Applied Microbiology

Different mechanisms of protein immobilization on glutaraldehyde activated supports:: Effect of support activation and immobilization conditions

Lorena Betancor et al.

ENZYME AND MICROBIAL TECHNOLOGY (2006)

Add to Collection
Article Biotechnology & Applied Microbiology

Purification and identification of different lipases contained in PPL commercial extracts:: A minor contaminant is the main responsible of most esterasic activity

Rosa L. Segura et al.

ENZYME AND MICROBIAL TECHNOLOGY (2006)

Add to Collection
Article Biotechnology & Applied Microbiology

CLEAs of lipases and poly-ionic polymers:: A simple way of preparing stable biocatalysts with improved properties

Lorena Wilson et al.

ENZYME AND MICROBIAL TECHNOLOGY (2006)

Add to Collection
Article Biotechnology & Applied Microbiology

Glyoxyl agarose:: A fully inert and hydrophilic support for immobilization and high stabilization of proteins

Cesar Mateo et al.

ENZYME AND MICROBIAL TECHNOLOGY (2006)

Add to Collection
Article Biotechnology & Applied Microbiology

One step purification-immobilization of fuculose-1-phosphate aldolase, a class II DHAP dependent aldolase, by using metal-chelate supports

Ines Ardao et al.

ENZYME AND MICROBIAL TECHNOLOGY (2006)

Add to Collection
Article Biotechnology & Applied Microbiology

Glyoxyl agarose as a new chromatographic matrix

V Grazu et al.

ENZYME AND MICROBIAL TECHNOLOGY (2006)

Add to Collection
Article Biochemistry & Molecular Biology

Stabilisation of oxygen-labile nitrilases via co-aggregation with poly (ethyleneimine)

C Mateo et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2006)

Add to Collection
Article Biophysics

Detecting minimal traces of DNA using DNA covalently attached to superparamagnetic nanoparticles and direct PCR-ELISA

M Fuentes et al.

BIOSENSORS & BIOELECTRONICS (2006)

Add to Collection
Review Biotechnology & Applied Microbiology

Kinetics of beta-lactam antibiotics synthesis by penicillin G acylase (PGA) from the viewpoint of the industrial enzymatic reactor optimization

RC Giordano et al.

BIOTECHNOLOGY ADVANCES (2006)

Add to Collection
Article Engineering, Environmental

Internal mass transfer effect on biodegradation of phenol by Ca-alginate immobilized Ralstonia eutropha

AY Dursun et al.

JOURNAL OF HAZARDOUS MATERIALS (2005)

Add to Collection
Article Biotechnology & Applied Microbiology

Protein-coated microcrystals for use in organic solvents: Application to oxidoreductases

M Kreiner et al.

BIOTECHNOLOGY LETTERS (2005)

Add to Collection
Review Chemistry, Physical

Ordered mesoporous materials for bioadsorption and biocatalysis

M Hartmann

CHEMISTRY OF MATERIALS (2005)

Add to Collection
Article Biotechnology & Applied Microbiology

Some special features of glyoxyl supports to immobilize proteins

C Mateo et al.

ENZYME AND MICROBIAL TECHNOLOGY (2005)

Add to Collection
Article Biotechnology & Applied Microbiology

Effects of internal mass transfer and product inhibition on a simulated immobilized enzyme-catalyzed reactor for lactose hydrolysis

AE Al-Muftah et al.

BIOCHEMICAL ENGINEERING JOURNAL (2005)

Add to Collection
Article Biotechnology & Applied Microbiology

Lipase-lipase interactions as a new tool to immobilize and modulate the lipase properties

JM Palomo et al.

ENZYME AND MICROBIAL TECHNOLOGY (2005)

Add to Collection
Article Biochemistry & Molecular Biology

Advantages of the pre-immobilization of enzymes on porous supports for their entrapment in sol-gels

L Betancor et al.

BIOMACROMOLECULES (2005)

Add to Collection
Article Biochemistry & Molecular Biology

Secondary structure, conformational stability and glycosylation of a recombinant Candida rugosa lipase studied by Fourier-transform infrared spectroscopy

A Natalello et al.

BIOCHEMICAL JOURNAL (2005)

Add to Collection
Article Biophysics

Preparation of inert magnetic nano-particles for the directed immobilization of antibodies

M Fuentes et al.

BIOSENSORS & BIOELECTRONICS (2005)

Add to Collection
Review Chemistry, Medicinal

Application of lipases in kinetic resolution of racemates

A Ghanem et al.

CHIRALITY (2005)

Add to Collection
Review Chemistry, Multidisciplinary

Reaction progress kinetic analysis: A powerful methodology for mechanistic studies of complex catalytic reactions

DG Blackmond

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION (2005)

Add to Collection
Article Biochemistry & Molecular Biology

Crosslinked enzyme crystals of glucoamylase as a potent catalyst for biotransformations

TE Abraham et al.

CARBOHYDRATE RESEARCH (2004)

Add to Collection
Article Biotechnology & Applied Microbiology

Stabilization of penicillin G acylase from Escherichia coli:: Site-directed mutagenesis of the protein surface to increase multipoint covalent attachment

O Abian et al.

APPLIED AND ENVIRONMENTAL MICROBIOLOGY (2004)

Add to Collection
Article Biotechnology & Applied Microbiology

Preparation, optimization, and structures of cross-linked enzyme aggregates (CLEAs)

R Schoevaart et al.

BIOTECHNOLOGY AND BIOENGINEERING (2004)

Add to Collection
Article Chemistry, Applied

Synthesis of 2′-deoxynucleosides by transglycosylation with new immobilized and stabilized uridine phosphorylase and purine nucleoside phosphorylase

D Ubiali et al.

ADVANCED SYNTHESIS & CATALYSIS (2004)

Add to Collection
Article Biochemical Research Methods

Determination of protein-protein interactions through aldehyde-dextran intermolecular cross-linking

M Fuentes et al.

PROTEOMICS (2004)

Add to Collection
Article Biotechnology & Applied Microbiology

Immobilization of lactase from Kluyveromyces lactis greatly reduces the inhibition promoted by glucose.: Full hydrolysis of lactose in milk

C Mateo et al.

BIOTECHNOLOGY PROGRESS (2004)

Add to Collection
Article Chemistry, Physical

Molecular simulation studies of the orientation and conformation of cytochrome c adsorbed on self-assembled monolayers

J Zhou et al.

JOURNAL OF PHYSICAL CHEMISTRY B (2004)

Add to Collection
Article Biotechnology & Applied Microbiology

Encapsulation of crosslinked penicillin G acylase aggregates in lentikats:: Evaluation of a novel biocatalyst in organic media

L Wilson et al.

BIOTECHNOLOGY AND BIOENGINEERING (2004)

Add to Collection
Article Biochemical Research Methods

Use of immobilized lipases for lipase purification via specific lipase-lipase interactions

JM Palomo et al.

JOURNAL OF CHROMATOGRAPHY A (2004)

Add to Collection
Article Biochemistry & Molecular Biology

Immobilization and stabilization of recombinant multimeric uridine and purine nucleoside phosphorylases from Bacillus subtilis

S Rocchietti et al.

BIOMACROMOLECULES (2004)

Add to Collection
Article Biochemistry & Molecular Biology

Co-aggregation of penicillin G acylase and polyionic polymers:: An easy methodology to prepare enzyme biocatalysts stable in organic media

L Wilson et al.

BIOMACROMOLECULES (2004)

Add to Collection
Article Biotechnology & Applied Microbiology

The immobilization of a thermophilic β-galactosidase on Sepabeads supports decreases product inhibition -: Complete hydrolysis of lactose in dairy products

BCC Pessela et al.

ENZYME AND MICROBIAL TECHNOLOGY (2003)

Add to Collection
Article Chemistry, Multidisciplinary

Controlling the aqueous miscibility of ionic liquids: Aqueous biphasic systems of water-miscible ionic liquids and water-structuring salts for recycle, metathesis, and separations

KE Gutowski et al.

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY (2003)

Add to Collection
Article Chemistry, Multidisciplinary

Nanoscale site-specific immobilization of proteins through electroactivated disulfide exchange

E Pavlovic et al.

NANO LETTERS (2003)

Add to Collection
Article Biotechnology & Applied Microbiology

Use of physicochemical tools to determine the choice of optimal enzyme:: Stabilization of D-amino acid oxidase

L Betancor et al.

BIOTECHNOLOGY PROGRESS (2003)

Add to Collection
Article Biochemistry & Molecular Biology

Epoxy-amino groups:: A new tool for improved immobilization of proteins by the epoxy method

C Mateo et al.

BIOMACROMOLECULES (2003)

Add to Collection
Article Biotechnology & Applied Microbiology

Self-assembly of Pseudomonas fluorescens lipase into bimolecular aggregates dramatically affects functional properties

G Fernández-Lorente et al.

BIOTECHNOLOGY AND BIOENGINEERING (2003)

Add to Collection
Article Biochemistry & Molecular Biology

Solid-phase handling of hydrophobins:: Immobilized hydrophobins as a new tool to study lipases

JM Palomo et al.

BIOMACROMOLECULES (2003)

Add to Collection
Article Chemistry, Physical

Using sugar and amino acid additives to stabilize enzymes within sol-gel derived silica

JD Brennan et al.

CHEMISTRY OF MATERIALS (2003)

Add to Collection
Review Biotechnology & Applied Microbiology

Sonobioreactors: using ultrasound for enhanced microbial productivity

Y Chisti

TRENDS IN BIOTECHNOLOGY (2003)

Add to Collection
Article Chemistry, Organic

Enzymatic transformations. Immobilized A-niger epoxide hydrolase as a novel biocatalytic tool for repeated-batch hydrolytic kinetic resolution of epoxides

C Mateo et al.

ORGANIC & BIOMOLECULAR CHEMISTRY (2003)

Add to Collection
Article Biochemistry & Molecular Biology

General trend of lipase to self-assemble giving bimolecular aggregates greatly modifies the enzyme functionality

JM Palomo et al.

BIOMACROMOLECULES (2003)

Add to Collection
Article Chemistry, Inorganic & Nuclear

Enzymatic production of (3S,4R)-(-)-4-(4′-fluorophenyl)-6-oxo-piperidin-3-carboxylic acid using a commercial preparation of lipase A from Candida antarctica:: the role of a contaminant esterase

JM Palomo et al.

TETRAHEDRON-ASYMMETRY (2002)

Add to Collection
Article Biotechnology & Applied Microbiology

Modulation of the enantioselectivity of lipases via controlled immobilization and medium engineering: hydrolytic resolution of mandelic acid esters

JM Palomo et al.

ENZYME AND MICROBIAL TECHNOLOGY (2002)

Add to Collection
Article Chemistry, Applied

EUPERGIT oxirane acrylic beads: How to make enzymes fit for biocatalysis

T Boller et al.

ORGANIC PROCESS RESEARCH & DEVELOPMENT (2002)

Add to Collection
Article Biotechnology & Applied Microbiology

Epoxy sepabeads:: A novel epoxy support for stabilization of industrial enzymes via very intense multipoint covalent attachment

C Mateo et al.

BIOTECHNOLOGY PROGRESS (2002)

Add to Collection
Article Chemistry, Multidisciplinary

Enzyme-coated micro-crystals: a 1-step method for high activity biocatalyst preparation

M Kreiner et al.

CHEMICAL COMMUNICATIONS (2001)

Add to Collection
Article Biochemical Research Methods

Affinity chromatography of polyhistidine tagged enzymes - New dextran-coated immobilized metal ion affinity chromatography matrices for prevention of undesired multipoint adsorptions

C Mateo et al.

JOURNAL OF CHROMATOGRAPHY A (2001)

Add to Collection
Article Biotechnology & Applied Microbiology

Direct measurement of pH profiles in immobilized enzyme carriers during kinetically controlled synthesis using CLSM

AC Spiess et al.

BIOTECHNOLOGY PROGRESS (2001)

Add to Collection
Article Biochemistry & Molecular Biology

Biotransformations catalyzed by multimeric enzymes:: Stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions

R Fernández-Lafuente et al.

BIOMACROMOLECULES (2001)

Add to Collection
Article Biochemistry & Molecular Biology

Effect of modulation of enzyme inactivation on temperature optimization for reactor operation with chitin-immobilized lactase

A Illanes et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2001)

Add to Collection
Article Biochemistry & Molecular Biology

Diffusion limitation causes decreased enantioselectivity of esterification of 2-butanol by immobilized Candida antarctica lipase B

MJJ Litjens et al.

BIOCATALYSIS AND BIOTRANSFORMATION (2001)

Add to Collection
Article Biochemistry & Molecular Biology

Multifunctional epoxy supports:: A new tool to improve the covalent immobilization of proteins.: The promotion of physical adsorptions of proteins on the supports before their covalent linkage

C Mateo et al.

BIOMACROMOLECULES (2000)

Add to Collection
Article Biochemistry & Molecular Biology

Eupergit® C, a carrier for immobilization of enzymes of industrial potential

E Katchalski-Katzir et al.

JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC (2000)

Add to Collection
Article Biochemistry & Molecular Biology

Performance of a continuous Taylor-Couette-Poiseuille vortex flow enzymic reactor with suspended particles

RLC Giordano et al.

PROCESS BIOCHEMISTRY (2000)

Add to Collection
Article Chemistry, Organic

Cross-linked enzyme aggregates: A simple and effective method for the immobilization of penicillin acylase

LQ Cao et al.

ORGANIC LETTERS (2000)

Add to Collection
Article Biotechnology & Applied Microbiology

Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment

C Mateo et al.

ENZYME AND MICROBIAL TECHNOLOGY (2000)

Add to Collection
Article Biochemistry & Molecular Biology

Production and characterisation of cross-linked enzyme crystals (CLECs (R)) for application as process scale biocatalysts

JD Vaghjiani et al.

BIOCATALYSIS AND BIOTRANSFORMATION (2000)

Add to Collection
Webinars Posters Questions Hubs Funding Journals Papers Connect Collections Reviewers About Us FAQs Mobile App Privacy Policy Terms of Use
© Peeref 2019-2024. All rights reserved.