Journal
BURNS & TRAUMA
Volume 10, Issue -, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/burnst/tkac015
Keywords
Hypertrophic scar; Glycolysis; Fibrosis; IR780; Activated fibroblast
Categories
Funding
- National Natural Science Foundation of China [82172222, 82102328]
- Shanghai Municipal Education Commission Gaofeng Clinical Medicine Grant Support [20152227]
- Clinical Multi-Disciplinary Team Research Program of ninth People's Hospital, Shanghai Jiao Tong University School of Medicine [20171-007]
- Shanghai HealthIndustry Clinical Research Special Project [20204Y0443]
- Shanghai Municipal Key ClinicalS pecialty [shslczdzk00901]
- Cross Research Project of Ninth People's Hospital, ShanghaiJiao Tong University School of Medicine [JYJC202009]
Ask authors/readers for more resources
This study confirms the link between augmented glycolysis and fibrotic activity in hypertrophic scars and proposes a method of controlling scar formation through regulation of glycolysis. The introduction of IR780 effectively down-regulated glycolysis and suppressed fibrotic activity, both in vitro and in vivo, by targeting activated fibroblasts.
Background Hypertrophic scars (HS) represent one of the most common clinical challenges due to unsatisfactory therapeutic results. HS formation is associated with the abnormal activation of fibroblasts and their excessive fibrotic behavior. Glycolysis dysregulation has been shown to participate in the incidence and progression of various fibrotic diseases and shows potential as a means of controlling HS formation. This work aimed to discuss the impact of augmented glycolysis on HS and to propose a method for controlling HS formation through glycolysis regulation. Methods Here, augmented glycolysis was confirmed together with enhanced fibrotic activity in both HS fibroblasts (HFs) and HS tissues, and the suppression of glycolysis also attenuated fibroblast activation. We also introduced IR780, a heptamethine cyanine dye, to regulate glycolysis for the control of HS formation. Results In vitro, cell studies indicated that IR780 significantly down-regulated glycolysis and suppressed the fibrotic activity of HFs. In vivo, the intralesional injection of IR780 into rabbit HS models led to the downregulation of glycolysis and the control of HS formation. Furthermore, IR780 accumulated preferentially in activated fibroblasts in both in vitro and in vivo studies, and thus specifically downregulated glycolysis and efficiently controlled fibrosis by targeting activated fibroblasts. Conclusions This work identified a strategy for controlling fibrosis and HS formation from the perspective of glycolysis regulation with IR780 targeting of activated fibroblasts.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available