Journal
ANAIS DA ACADEMIA BRASILEIRA DE CIENCIAS
Volume 94, Issue 4, Pages -Publisher
ACAD BRASILEIRA DE CIENCIAS
DOI: 10.1590/0001-3765202220200515
Keywords
Clone; growth regulators; micropropagation; rubber tree; seeds
Categories
Funding
- Coordination for the Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES)-Brazil [52]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Brazil)
- Fundacao de Amparo a Pesquisa do Estado do Amazonas (FAPEAM)
- CNPq
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This study examined the effect of growth regulators on the germination and seedling establishment of Hevea brasiliensis. The results showed that different genotypes responded differently to the growth regulators, and the protein profiles of the seedlings varied in the presence of different growth regulators.
The main factors governing Hevea brasiliensis germination and seedling establishment remains unclear. We examined the effect of growth regulators Indole 3-Acetic Acid (IAA) and 6-Benzylaminopurine (BAP), and their interactions on germination and the development of mature zygotic embryos (MZE) and protein profile of Hevea brasiliensis seedlings from wild and cultivated (clone PB 250) genotypes. Embryonic axes excised from seeds (wild and clone PB 250) were inoculated in Murashige and Skoog medium (control) and supplemented with IAA (3 mu M) and BAP (6 mu M) individually and their combination (3 mu M IAA + 6 mu M BAP). For both genotypes, the mature embryos displayed a high percentage of germination and establishment, and the seedlings were characterized by protein bands ranging from 7 to 30 kDa. Notably, the wild genotype showed proteins in the 14 kDa range, which may be associated with one of the major rubber elongation factors (REF). The wild and clone genotypes presented different behavior and strategies in relation to the protein profile in the presence of different growth regulators. Although the latex biosynthetic pathway and its mechanisms of regulation still remain largely unknown, our results aid in our understanding of the dynamics of proteins in different rubber tree clones in vitro.
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