Journal
JOURNAL OF KOREAN MEDICAL SCIENCE
Volume 37, Issue 28, Pages -Publisher
KOREAN ACAD MEDICAL SCIENCES
DOI: 10.3346/jkms.2022.37.e220
Keywords
Gastric Cancer; Organoid; Endoscopy; Biopsy; Precision Medicine
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Funding
- Bio & Medical Technology Development Program of the National Research Foundation (NRF) - Ministry of Science ICT [NRF-2017M3A9F3047495]
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This study successfully established and cultured gastric cancer patient-derived organoids (PDOs) and verified their origin and tumor characteristics through histopathological examination and staining methods. The results of this study demonstrate that gastric cancer PDO models exhibit the characteristics of primary tumors and have potential applications in drug screening and development.
Cancer organoids are three-dimensional mini-organ analogues derived from cancer tissues and have been proposed as models capable of simulating the structure and function of human organs and tissues in vitro. We sought to establish gastric cancer patient-derived organoids (PDOs) from tissues obtained by endoscopic biopsies. Gastric cancer-PD Os were successfully established and cultured from cancer tissues with gastric adenocarcinoma by endoscopic biopsies. To confirm that gastric cancer-PDOs were derived from cancer tissue, the consistency of the original cancer tissue was assessed by histopathological examination. As a result, it was confirmed that the shape and internal structure of gastric cancer-PD O were derived from the original gastric cancer cells, and the tumor specificity of gastric cancer-PDO was confirmed through Periodic acid-Schiff (PAS) and polyclonal carcinoembryonic antigen antibody staining. These results demonstrate that gastric cancer-PDO models show the characteristics of primary tumors and have potential for drug screening and providing a medicine
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