Journal
GENOME BIOLOGY
Volume 23, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s13059-022-02730-x
Keywords
Prime editing; Efficiency; Engineering; Rice; Precise editing
Funding
- Science and Technology Major Projects of Anhui Province [2021d06050002, 202003a06020009]
- National Natural Science Foundation of China [U19A2022, 32000284]
- Natural Science Foundation of Anhui Province [2108085Y07, 2208085Y11, 2008085QC101, 2008085MC71]
- University Synergy Innovation Program of Anhui Province [GXXT-2021-056]
- Open Research Fund Program of Anhui Province Key Laboratory of Rice Genetics and Breeding [SDKF-2021-01, SDKF-2020-03]
- Joint Project of Elite Varieties Breeding of Anhui Province
Ask authors/readers for more resources
In this study, an enhanced plant Prime Editor 2 system (enpPE2) is developed to improve editing efficiency. The results demonstrate that enpPE2 is a robust and powerful tool for precise modification of plant genomes.
Low efficiency has seriously restricted the application of prime editing (PE) systems in plants. In this study, we develop an enhanced plant prime editor 2 system, enpPE2, by stacking various optimization strategies, including updating the PE architecture to PEmax and expressing engineered pegRNA with a structured motif under the control of a composite promoter. In T-0 rice plants, enpPE2 exhibits editing frequencies of 64.58% to 77.08%, which are much higher than the frequencies with unmodified pPE2. Our results indicate that the enpPE2 system provides a robust and powerful tool for the precise modification of plant genomes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available
Share Paper
