Journal
BIOENGINEERING-BASEL
Volume 9, Issue 7, Pages -Publisher
MDPI
DOI: 10.3390/bioengineering9070313
Keywords
thermoplastic polymers; HIPS; sacrificial matrix; perfusion; dense collagen hydrogel
Funding
- Sorbonne Universite
- CNRS
- INSERM
- AFM-Telethon [22142]
- Ile-de-France Region
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A novel strategy was reported in this study to create a perfusion system within dense collagen hydrogels using 3D printed thermoplastic filaments. The perfusion network generated by the dissolution of sacrificial matrix did not alter the physical or chemical properties of the collagen hydrogel and was associated with increased fibroblast survival.
Dense collagen hydrogels are promising biomaterials for several tissue-engineering applications. They exhibit high mechanical properties, similar to physiological extracellular matrices, and do not shrink under cellular activity. However, they suffer from several drawbacks, such as weak nutrient and O-2 diffusion, impacting cell survival. Here, we report a novel strategy to create a perfusion system within dense and thick collagen hydrogels to promote cell viability. The 3D printing of a thermoplastic filament (high-impact polystyrene, HIPS) with a three-wave shape is used to produce an appropriate sacrificial matrix. The HIPS thermoplastic polymer allows for good shape fidelity of the filament and does not collapse under the mechanical load of the collagen solution. After the collagen gels around the filament and dissolves, a channel is generated, allowing for adequate and rapid hydrogel perfusion. The dissolution process does not alter the collagen hydrogel's physical or chemical properties, and the perfusion is associated with an increased fibroblast survival. Here, we report the novel utilization of thermoplastics to generate a perfusion network within biomimetic collagen hydrogels.
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