Journal
REGENERATIVE BIOMATERIALS
Volume 9, Issue -, Pages -Publisher
OXFORD UNIV PRESS
DOI: 10.1093/rb/rbac039
Keywords
amelogenin; peptide hydrogel; odontogenic differentiation; human dental pulp cells
Categories
Funding
- National Science Fund for Excellent Young Scholars [T2122019]
- National Natural Science Foundation of China [51973096, 51773097]
- Natural Science Foundation of Tianjin City [18JCYBJC27000]
- Technology Research and Development Program of Tianjin [20YFZCSY00830]
- Tianjin Key Medical Discipline(Specialty) Construction Project [2021-516]
- Science and Technology Project of Tianjin Health Commission [ZD20016]
- Key Laboratory of Bioactive Materials, Ministry of Education [NKBM2019-001, NKBM-2019-002]
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This study constructed amelogenin self-assembling peptide hydrogels and investigated their effects on the odontogenic differentiation of human dental pulp cells (HDPCs). The results showed that the D-gel exhibited the strongest enhancement effects and induced odontogenic differentiation through the MAPK-ERK1/2 and transforming growth factor/smad pathways. This study demonstrated the potential of amelogenin peptide hydrogel in dentine-pulp complex regeneration.
Amelogenin can induce odontogenic differentiation of human dental pulp cells (HDPCs), which has great potential and advantages in dentine-pulp complex regeneration. However, the unstability of amelogenin limits its further application. This study constructed amelogenin self-assembling peptide hydrogels (L-gel or D-gel) by heating-cooling technique, investigated the effects of these hydrogels on the odontogenic differentiation of HDPCs and explored the underneath mechanism. The critical aggregation concentration, conformation, morphology, mechanical property and biological stability of the hydrogels were characterized, respectively. The effects of the hydrogels on the odontogenic differentiation of HDPCs were evaluated via alkaline phosphatase activity measurement, quantitative reverse transcription polymerase chain reaction, western blot, Alizarin red staining and scanning electron microscope. The mechanism was explored via signaling pathway experiments. Results showed that both the L-gel and D-gel stimulated the odontogenic differentiation of HDPCs on both Day 7 and Day 14, while the D-gel showed the highest enhancement effects. Meanwhile, the D-gel promoted calcium accumulation and mineralized matrix deposition on Day 21. The D-gel activated MAPK-ERK1/2 pathways in HDPCs and induced the odontogenic differentiation via ERK1/2 and transforming growth factor/smad pathways. Overall, our study demonstrated that the amelogenin peptide hydrogel stimulated the odontogenic differentiation and enhanced mineralization, which held big potential in the dentine-pulp complex regeneration.
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